Experiment: LB_plus_SM_buffer with EIP16_phage 0.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3771 and GFF3772 overlap by 1 nucleotides GFF3772 and GFF3773 are separated by 16 nucleotides GFF3773 and GFF3774 are separated by 64 nucleotides
GFF3771 - Fructose-specific phosphocarrier protein HPr (EC 2.7.1.69) / PTS system, fructose-specific IIA component (EC 2.7.1.69), at 37,497 to 38,627
GFF3771
GFF3772 - 1-phosphofructokinase (EC 2.7.1.56), at 38,627 to 39,565
GFF3772
GFF3773 - PTS system, fructose-specific IIB component (EC 2.7.1.69) / PTS system, fructose-specific IIC component (EC 2.7.1.69), at 39,582 to 41,270
GFF3773
GFF3774 - Endonuclease IV (EC 3.1.21.2), at 41,335 to 42,192
GFF3774
Position (kb) 39
40
41
42 Strain fitness (log2 ratio) -2
-1
0
1 at 38.666 kb on + strand at 39.118 kb on + strand, within GFF3772 at 39.370 kb on - strand, within GFF3772 at 39.446 kb on - strand, within GFF3772 at 39.549 kb on + strand at 39.598 kb on + strand at 39.630 kb on + strand at 39.631 kb on - strand at 40.177 kb on + strand, within GFF3773 at 40.284 kb on + strand, within GFF3773 at 40.284 kb on + strand, within GFF3773 at 40.285 kb on - strand, within GFF3773 at 40.441 kb on - strand, within GFF3773 at 40.563 kb on - strand, within GFF3773 at 40.685 kb on + strand, within GFF3773 at 40.787 kb on + strand, within GFF3773 at 40.787 kb on + strand, within GFF3773 at 40.890 kb on - strand, within GFF3773 at 40.890 kb on - strand, within GFF3773 at 40.890 kb on - strand, within GFF3773 at 41.045 kb on - strand, within GFF3773 at 41.058 kb on - strand, within GFF3773 at 41.160 kb on - strand at 41.160 kb on - strand at 41.451 kb on + strand, within GFF3774 at 41.656 kb on - strand, within GFF3774 at 41.661 kb on + strand, within GFF3774 at 41.682 kb on + strand, within GFF3774 at 41.683 kb on - strand, within GFF3774 at 41.736 kb on + strand, within GFF3774 at 41.800 kb on - strand, within GFF3774 at 41.970 kb on + strand, within GFF3774 at 42.186 kb on - strand at 42.186 kb on - strand at 42.226 kb on + strand at 42.226 kb on + strand at 42.227 kb on - strand at 42.267 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.875 MOI remove 38,666 + -2.7 39,118 + GFF3772 0.52 -2.4 39,370 - GFF3772 0.79 -1.1 39,446 - GFF3772 0.87 -0.9 39,549 + -0.2 39,598 + +0.2 39,630 + +0.1 39,631 - -0.1 40,177 + GFF3773 0.35 -0.3 40,284 + GFF3773 0.42 -0.5 40,284 + GFF3773 0.42 +0.0 40,285 - GFF3773 0.42 +1.0 40,441 - GFF3773 0.51 +0.2 40,563 - GFF3773 0.58 -0.8 40,685 + GFF3773 0.65 -0.0 40,787 + GFF3773 0.71 -0.6 40,787 + GFF3773 0.71 +0.1 40,890 - GFF3773 0.77 -0.6 40,890 - GFF3773 0.77 -0.2 40,890 - GFF3773 0.77 +0.9 41,045 - GFF3773 0.87 +0.9 41,058 - GFF3773 0.87 +0.1 41,160 - +1.1 41,160 - -0.9 41,451 + GFF3774 0.14 +0.2 41,656 - GFF3774 0.37 +0.9 41,661 + GFF3774 0.38 -0.7 41,682 + GFF3774 0.40 +0.2 41,683 - GFF3774 0.41 -0.2 41,736 + GFF3774 0.47 -0.6 41,800 - GFF3774 0.54 -1.5 41,970 + GFF3774 0.74 -1.0 42,186 - +0.4 42,186 - +0.4 42,226 + +0.0 42,226 + -0.2 42,227 - -0.0 42,267 + -0.3
Or see this region's nucleotide sequence