Experiment: LB_plus_SM_buffer with EIP16_phage 0.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3723 and GFF3724 are separated by 187 nucleotides GFF3724 and GFF3725 are separated by 219 nucleotides GFF3725 and GFF3726 are separated by 276 nucleotides GFF3726 and GFF3727 overlap by 1 nucleotides
GFF3723 - FKBP-type peptidyl-prolyl cis-trans isomerase SlyD (EC 5.2.1.8), at 28,037 to 28,627
GFF3723
GFF3724 - Protein SlyX, at 28,815 to 29,033
GFF3724
GFF3725 - FKBP-type peptidyl-prolyl cis-trans isomerase FkpA precursor (EC 5.2.1.8), at 29,253 to 30,071
GFF3725
GFF3726 - YheO-like PAS domain, at 30,348 to 31,070
GFF3726
GFF3727 - tRNA 5-methylaminomethyl-2-thiouridine synthase TusD, at 31,070 to 31,456
GFF3727
Position (kb) 29
30
31 Strain fitness (log2 ratio) -2
-1
0
1
2 at 28.277 kb on - strand, within GFF3723 at 28.437 kb on + strand, within GFF3723 at 28.662 kb on + strand at 28.686 kb on - strand at 28.686 kb on - strand at 28.686 kb on - strand at 28.686 kb on - strand at 28.686 kb on - strand at 28.686 kb on - strand at 28.686 kb on - strand at 28.689 kb on + strand at 28.689 kb on + strand at 28.690 kb on - strand at 28.690 kb on - strand at 28.693 kb on + strand at 28.693 kb on + strand at 28.694 kb on - strand at 28.754 kb on - strand at 28.768 kb on - strand at 28.819 kb on + strand at 28.857 kb on - strand, within GFF3724 at 28.962 kb on + strand, within GFF3724 at 29.038 kb on + strand at 29.047 kb on - strand at 29.047 kb on - strand at 29.047 kb on - strand at 29.047 kb on - strand at 29.047 kb on - strand at 29.070 kb on + strand at 29.112 kb on - strand at 29.155 kb on + strand at 29.169 kb on - strand at 29.208 kb on - strand at 29.211 kb on + strand at 29.288 kb on + strand at 29.289 kb on - strand at 29.376 kb on - strand, within GFF3725 at 29.688 kb on - strand, within GFF3725 at 29.701 kb on - strand, within GFF3725 at 29.741 kb on + strand, within GFF3725 at 29.805 kb on + strand, within GFF3725 at 29.980 kb on + strand, within GFF3725 at 30.126 kb on + strand at 30.126 kb on + strand at 30.126 kb on + strand at 30.126 kb on + strand at 30.126 kb on + strand at 30.129 kb on + strand at 30.129 kb on + strand at 30.130 kb on - strand at 30.217 kb on + strand at 30.217 kb on + strand at 30.241 kb on - strand at 30.268 kb on - strand at 30.428 kb on + strand, within GFF3726 at 30.473 kb on + strand, within GFF3726 at 30.475 kb on + strand, within GFF3726 at 30.475 kb on + strand, within GFF3726 at 30.476 kb on - strand, within GFF3726 at 30.554 kb on + strand, within GFF3726 at 30.554 kb on + strand, within GFF3726 at 30.554 kb on + strand, within GFF3726 at 30.599 kb on + strand, within GFF3726 at 30.599 kb on + strand, within GFF3726 at 30.629 kb on + strand, within GFF3726 at 30.727 kb on + strand, within GFF3726 at 30.727 kb on + strand, within GFF3726 at 30.727 kb on + strand, within GFF3726 at 30.962 kb on + strand, within GFF3726 at 30.982 kb on + strand, within GFF3726 at 31.017 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.875 MOI remove 28,277 - GFF3723 0.41 -0.9 28,437 + GFF3723 0.68 -0.6 28,662 + -0.2 28,686 - +2.2 28,686 - -0.1 28,686 - -0.8 28,686 - -0.2 28,686 - +1.5 28,686 - -0.1 28,686 - -0.1 28,689 + -0.5 28,689 + -0.1 28,690 - -0.1 28,690 - -0.3 28,693 + -0.2 28,693 + -0.7 28,694 - +0.0 28,754 - -0.1 28,768 - -0.3 28,819 + -0.2 28,857 - GFF3724 0.19 -0.5 28,962 + GFF3724 0.67 +0.9 29,038 + +0.3 29,047 - -0.3 29,047 - -1.2 29,047 - +0.4 29,047 - +0.8 29,047 - -0.2 29,070 + -0.1 29,112 - -0.4 29,155 + +0.3 29,169 - -0.2 29,208 - -0.7 29,211 + +0.2 29,288 + -2.1 29,289 - -1.8 29,376 - GFF3725 0.15 -2.0 29,688 - GFF3725 0.53 -0.8 29,701 - GFF3725 0.55 +0.8 29,741 + GFF3725 0.60 -2.0 29,805 + GFF3725 0.67 -1.7 29,980 + GFF3725 0.89 -1.2 30,126 + -0.1 30,126 + +0.2 30,126 + +0.7 30,126 + +0.1 30,126 + -0.3 30,129 + +0.4 30,129 + +0.4 30,130 - -0.5 30,217 + +0.1 30,217 + +0.1 30,241 - -0.2 30,268 - +1.3 30,428 + GFF3726 0.11 +0.1 30,473 + GFF3726 0.17 +0.1 30,475 + GFF3726 0.18 -0.6 30,475 + GFF3726 0.18 +0.3 30,476 - GFF3726 0.18 +0.9 30,554 + GFF3726 0.28 +0.9 30,554 + GFF3726 0.28 -0.4 30,554 + GFF3726 0.28 +0.4 30,599 + GFF3726 0.35 +0.7 30,599 + GFF3726 0.35 +0.1 30,629 + GFF3726 0.39 -1.5 30,727 + GFF3726 0.52 +0.3 30,727 + GFF3726 0.52 -0.2 30,727 + GFF3726 0.52 -0.8 30,962 + GFF3726 0.85 +0.2 30,982 + GFF3726 0.88 -0.2 31,017 + -0.3
Or see this region's nucleotide sequence