Experiment: LB_plus_SM_buffer with EIP16_phage 0.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF358 and GFF359 are separated by 54 nucleotides GFF359 and GFF360 are separated by 64 nucleotides GFF360 and GFF361 are separated by 42 nucleotides
GFF358 - DNA-directed RNA polymerase omega subunit (EC 2.7.7.6), at 79,027 to 79,302
GFF358
GFF359 - Guanylate kinase (EC 2.7.4.8), at 79,357 to 79,980
GFF359
GFF360 - hypothetical protein, at 80,045 to 80,194
GFF360
GFF361 - DNA ligase (EC 6.5.1.2), LigB, at 80,237 to 81,922
GFF361
Position (kb)
80
81 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 79.064 kb on - strand, within GFF358 at 79.303 kb on - strand at 79.307 kb on - strand at 79.414 kb on - strand at 80.029 kb on - strand at 80.029 kb on - strand at 80.029 kb on - strand at 80.033 kb on + strand at 80.056 kb on - strand at 80.175 kb on - strand, within GFF360 at 80.195 kb on + strand at 80.254 kb on + strand at 80.542 kb on + strand, within GFF361 at 80.565 kb on + strand, within GFF361 at 80.565 kb on + strand, within GFF361 at 80.628 kb on + strand, within GFF361 at 80.728 kb on + strand, within GFF361 at 80.728 kb on + strand, within GFF361 at 80.729 kb on - strand, within GFF361 at 80.769 kb on + strand, within GFF361 at 80.788 kb on - strand, within GFF361 at 80.803 kb on + strand, within GFF361 at 80.803 kb on + strand, within GFF361 at 80.804 kb on - strand, within GFF361 at 80.902 kb on + strand, within GFF361 at 80.946 kb on + strand, within GFF361 at 80.987 kb on + strand, within GFF361 at 80.987 kb on + strand, within GFF361 at 80.989 kb on + strand, within GFF361 at 81.075 kb on + strand, within GFF361 at 81.082 kb on - strand, within GFF361 at 81.082 kb on - strand, within GFF361 at 81.152 kb on + strand, within GFF361
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.875 MOI remove 79,064 - GFF358 0.13 -1.1 79,303 - -2.3 79,307 - -3.0 79,414 - +2.7 80,029 - -1.0 80,029 - -0.3 80,029 - +0.0 80,033 + -0.8 80,056 - +1.1 80,175 - GFF360 0.87 -0.7 80,195 + +0.2 80,254 + -0.9 80,542 + GFF361 0.18 +0.0 80,565 + GFF361 0.19 +0.8 80,565 + GFF361 0.19 -0.1 80,628 + GFF361 0.23 +0.5 80,728 + GFF361 0.29 +0.1 80,728 + GFF361 0.29 -0.3 80,729 - GFF361 0.29 +0.1 80,769 + GFF361 0.32 +0.6 80,788 - GFF361 0.33 +0.2 80,803 + GFF361 0.34 +0.0 80,803 + GFF361 0.34 +0.3 80,804 - GFF361 0.34 +1.2 80,902 + GFF361 0.39 +2.2 80,946 + GFF361 0.42 -0.7 80,987 + GFF361 0.44 -1.0 80,987 + GFF361 0.44 -0.1 80,989 + GFF361 0.45 +0.2 81,075 + GFF361 0.50 +0.4 81,082 - GFF361 0.50 -0.3 81,082 - GFF361 0.50 +0.5 81,152 + GFF361 0.54 -0.0
Or see this region's nucleotide sequence