Experiment: LB_plus_SM_buffer with EIP16_phage 0.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3353 and GFF3354 are separated by 156 nucleotides GFF3354 and GFF3355 are separated by 3 nucleotides GFF3355 and GFF3356 are separated by 11 nucleotides GFF3356 and GFF3357 are separated by 26 nucleotides
GFF3353 - Flagellar basal-body P-ring formation protein FlgA, at 119,483 to 120,142
GFF3353
GFF3354 - Flagellar basal-body rod protein FlgB, at 120,299 to 120,715
GFF3354
GFF3355 - Flagellar basal-body rod protein FlgC, at 120,719 to 121,123
GFF3355
GFF3356 - Flagellar basal-body rod modification protein FlgD, at 121,135 to 121,833
GFF3356
GFF3357 - Flagellar hook protein FlgE, at 121,860 to 123,071
GFF3357
Position (kb)
120
121
122 Strain fitness (log2 ratio)
-1
0
1
2 at 120.146 kb on - strand at 120.179 kb on - strand at 120.207 kb on - strand at 120.210 kb on + strand at 120.211 kb on - strand at 120.261 kb on + strand at 120.262 kb on - strand at 120.298 kb on + strand at 120.401 kb on - strand, within GFF3354 at 120.589 kb on + strand, within GFF3354 at 120.593 kb on - strand, within GFF3354 at 120.598 kb on - strand, within GFF3354 at 120.598 kb on - strand, within GFF3354 at 120.601 kb on + strand, within GFF3354 at 120.602 kb on - strand, within GFF3354 at 120.950 kb on + strand, within GFF3355 at 120.986 kb on + strand, within GFF3355 at 120.987 kb on - strand, within GFF3355 at 121.052 kb on + strand, within GFF3355 at 121.053 kb on - strand, within GFF3355 at 121.064 kb on - strand, within GFF3355 at 121.135 kb on - strand at 121.140 kb on + strand at 121.152 kb on + strand at 121.395 kb on + strand, within GFF3356 at 121.395 kb on + strand, within GFF3356 at 121.395 kb on + strand, within GFF3356 at 121.395 kb on + strand, within GFF3356 at 121.577 kb on + strand, within GFF3356 at 121.613 kb on + strand, within GFF3356 at 121.614 kb on - strand, within GFF3356 at 121.635 kb on + strand, within GFF3356 at 121.672 kb on + strand, within GFF3356 at 121.792 kb on + strand at 121.839 kb on + strand at 121.840 kb on - strand at 121.840 kb on - strand at 121.845 kb on + strand at 121.925 kb on + strand at 121.947 kb on + strand at 121.956 kb on - strand at 121.956 kb on - strand at 121.982 kb on + strand, within GFF3357
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.875 MOI remove 120,146 - +0.6 120,179 - -0.2 120,207 - -0.0 120,210 + +0.4 120,211 - +0.8 120,261 + +0.9 120,262 - +1.1 120,298 + +1.2 120,401 - GFF3354 0.24 +0.6 120,589 + GFF3354 0.70 +2.2 120,593 - GFF3354 0.71 +0.8 120,598 - GFF3354 0.72 +0.2 120,598 - GFF3354 0.72 +0.2 120,601 + GFF3354 0.72 +0.7 120,602 - GFF3354 0.73 +0.7 120,950 + GFF3355 0.57 -1.7 120,986 + GFF3355 0.66 -0.0 120,987 - GFF3355 0.66 +2.1 121,052 + GFF3355 0.82 +1.0 121,053 - GFF3355 0.82 +1.1 121,064 - GFF3355 0.85 +1.4 121,135 - +1.0 121,140 + +0.0 121,152 + +1.1 121,395 + GFF3356 0.37 -0.1 121,395 + GFF3356 0.37 +0.0 121,395 + GFF3356 0.37 +0.8 121,395 + GFF3356 0.37 +1.0 121,577 + GFF3356 0.63 +1.0 121,613 + GFF3356 0.68 +0.1 121,614 - GFF3356 0.69 +1.1 121,635 + GFF3356 0.72 +1.1 121,672 + GFF3356 0.77 +0.9 121,792 + +0.2 121,839 + +0.2 121,840 - +0.7 121,840 - +2.0 121,845 + +1.0 121,925 + +1.0 121,947 + +1.7 121,956 - +1.6 121,956 - +1.7 121,982 + GFF3357 0.10 +1.0
Or see this region's nucleotide sequence