Experiment: LB_plus_SM_buffer with EIP16_phage 0.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3646 and GFF3312 are separated by 73 nucleotides GFF3312 and GFF3313 overlap by 2 nucleotides GFF3313 and GFF3314 are separated by 83 nucleotides GFF3314 and GFF3315 are separated by 23 nucleotides
GFF3646 - tRNA-Ser-GGA, at 86,793 to 86,880
GFF3646
GFF3312 - FIG01046704: hypothetical protein, at 86,954 to 87,118
GFF3312
GFF3313 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 87,117 to 88,055
GFF3313
GFF3314 - Putative hydrolase YcdX (EC 3.1.-.-), at 88,139 to 88,876
GFF3314
GFF3315 - Putative oxidoreductase component of anaerobic dehydrogenases; Functional role page for Chaperone protein TorD, at 88,900 to 89,454
GFF3315
Position (kb)
87
88
89 Strain fitness (log2 ratio)
-1
0
1
2 at 86.788 kb on - strand at 87.222 kb on - strand, within GFF3313 at 87.286 kb on + strand, within GFF3313 at 87.337 kb on + strand, within GFF3313 at 87.420 kb on + strand, within GFF3313 at 87.420 kb on + strand, within GFF3313 at 87.421 kb on - strand, within GFF3313 at 87.421 kb on - strand, within GFF3313 at 87.440 kb on - strand, within GFF3313 at 87.652 kb on + strand, within GFF3313 at 87.743 kb on + strand, within GFF3313 at 87.851 kb on - strand, within GFF3313 at 88.056 kb on + strand at 88.056 kb on + strand at 88.057 kb on - strand at 88.057 kb on - strand at 88.100 kb on - strand at 88.160 kb on - strand at 88.166 kb on - strand at 88.188 kb on - strand at 88.193 kb on - strand at 88.205 kb on + strand at 88.207 kb on + strand at 88.521 kb on + strand, within GFF3314 at 88.521 kb on + strand, within GFF3314 at 88.522 kb on - strand, within GFF3314 at 88.522 kb on - strand, within GFF3314 at 88.526 kb on - strand, within GFF3314 at 88.526 kb on - strand, within GFF3314 at 88.562 kb on - strand, within GFF3314 at 88.874 kb on + strand at 88.875 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.875 MOI remove 86,788 - -0.4 87,222 - GFF3313 0.11 +0.8 87,286 + GFF3313 0.18 -0.2 87,337 + GFF3313 0.23 +0.3 87,420 + GFF3313 0.32 -0.6 87,420 + GFF3313 0.32 +0.7 87,421 - GFF3313 0.32 +2.1 87,421 - GFF3313 0.32 +0.7 87,440 - GFF3313 0.34 +1.3 87,652 + GFF3313 0.57 -0.2 87,743 + GFF3313 0.67 +1.3 87,851 - GFF3313 0.78 +0.1 88,056 + -0.3 88,056 + +2.2 88,057 - +1.8 88,057 - +0.3 88,100 - -0.2 88,160 - +0.5 88,166 - -0.7 88,188 - -0.5 88,193 - +0.3 88,205 + -0.7 88,207 + +0.1 88,521 + GFF3314 0.52 -1.3 88,521 + GFF3314 0.52 -0.2 88,522 - GFF3314 0.52 +0.5 88,522 - GFF3314 0.52 -0.2 88,526 - GFF3314 0.52 +0.0 88,526 - GFF3314 0.52 -0.5 88,562 - GFF3314 0.57 -0.4 88,874 + -0.2 88,875 - -0.6
Or see this region's nucleotide sequence