Experiment: LB_plus_SM_buffer with EIP16_phage 0.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3272 and GFF3273 are separated by 7 nucleotides GFF3273 and GFF3274 are separated by 135 nucleotides GFF3274 and GFF3275 are separated by 332 nucleotides
GFF3272 - Putative two component system histidine kinase YedV, at 44,146 to 45,495
GFF3272
GFF3273 - Putative two-component system response regulator YedW, at 45,503 to 46,183
GFF3273
GFF3274 - 5-Hydroxyisourate Hydrolase (HIUase) (EC 3.5.2.17), at 46,319 to 46,729
GFF3274
GFF3275 - 4-hydroxyphenylacetate 3-monooxygenase, reductase component (EC 1.6.8.-), at 47,062 to 47,574
GFF3275
Position (kb) 45
46
47 Strain fitness (log2 ratio) -1
0
1
2 at 44.601 kb on + strand, within GFF3272 at 44.946 kb on + strand, within GFF3272 at 45.081 kb on + strand, within GFF3272 at 45.082 kb on - strand, within GFF3272 at 45.084 kb on + strand, within GFF3272 at 45.085 kb on - strand, within GFF3272 at 45.148 kb on + strand, within GFF3272 at 45.164 kb on - strand, within GFF3272 at 45.271 kb on + strand, within GFF3272 at 45.271 kb on + strand, within GFF3272 at 45.384 kb on + strand at 45.395 kb on + strand at 45.422 kb on + strand at 45.423 kb on - strand at 45.505 kb on - strand at 45.646 kb on + strand, within GFF3273 at 45.647 kb on - strand, within GFF3273 at 45.818 kb on - strand, within GFF3273 at 45.886 kb on + strand, within GFF3273 at 45.895 kb on - strand, within GFF3273 at 45.961 kb on + strand, within GFF3273 at 45.961 kb on + strand, within GFF3273 at 45.961 kb on + strand, within GFF3273 at 46.099 kb on - strand, within GFF3273 at 46.111 kb on - strand, within GFF3273 at 46.192 kb on + strand at 46.240 kb on + strand at 46.240 kb on + strand at 46.241 kb on - strand at 46.284 kb on + strand at 46.284 kb on + strand at 46.284 kb on + strand at 46.330 kb on + strand at 46.330 kb on + strand at 46.331 kb on - strand at 46.331 kb on - strand at 46.332 kb on + strand at 46.348 kb on - strand at 46.348 kb on - strand at 46.411 kb on + strand, within GFF3274 at 46.412 kb on - strand, within GFF3274 at 46.525 kb on + strand, within GFF3274 at 46.526 kb on - strand, within GFF3274 at 46.569 kb on - strand, within GFF3274 at 46.690 kb on - strand at 46.707 kb on - strand at 46.933 kb on + strand at 47.008 kb on - strand at 47.140 kb on - strand, within GFF3275 at 47.149 kb on - strand, within GFF3275
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.875 MOI remove 44,601 + GFF3272 0.34 +0.2 44,946 + GFF3272 0.59 -0.2 45,081 + GFF3272 0.69 -0.5 45,082 - GFF3272 0.69 +0.5 45,084 + GFF3272 0.69 -0.4 45,085 - GFF3272 0.70 +0.4 45,148 + GFF3272 0.74 -0.0 45,164 - GFF3272 0.75 -0.2 45,271 + GFF3272 0.83 -0.3 45,271 + GFF3272 0.83 +0.5 45,384 + +0.6 45,395 + +0.2 45,422 + -0.6 45,423 - -0.4 45,505 - -1.0 45,646 + GFF3273 0.21 +0.5 45,647 - GFF3273 0.21 -0.9 45,818 - GFF3273 0.46 +0.0 45,886 + GFF3273 0.56 -0.5 45,895 - GFF3273 0.58 -0.0 45,961 + GFF3273 0.67 +0.6 45,961 + GFF3273 0.67 +0.1 45,961 + GFF3273 0.67 -1.3 46,099 - GFF3273 0.88 -0.6 46,111 - GFF3273 0.89 -0.1 46,192 + -0.2 46,240 + +0.2 46,240 + -0.5 46,241 - -0.5 46,284 + +0.2 46,284 + +0.2 46,284 + -0.6 46,330 + -0.0 46,330 + +2.4 46,331 - -0.1 46,331 - -0.6 46,332 + +0.2 46,348 - -0.3 46,348 - -1.2 46,411 + GFF3274 0.22 +0.6 46,412 - GFF3274 0.23 -0.4 46,525 + GFF3274 0.50 -0.3 46,526 - GFF3274 0.50 +0.2 46,569 - GFF3274 0.61 +0.1 46,690 - -0.8 46,707 - -0.2 46,933 + -1.0 47,008 - +0.2 47,140 - GFF3275 0.15 +0.1 47,149 - GFF3275 0.17 -0.6
Or see this region's nucleotide sequence