Experiment: LB_plus_SM_buffer with EIP16_phage 0.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3240 and GFF3241 are separated by 14 nucleotides GFF3241 and GFF3242 are separated by 4 nucleotides
GFF3240 - COG0488: ATPase components of ABC transporters with duplicated ATPase domains, at 13,871 to 15,778
GFF3240
GFF3241 - Paraquat-inducible protein A, at 15,793 to 17,046
GFF3241
GFF3242 - Paraquat-inducible protein B, at 17,051 to 18,691
GFF3242
Position (kb) 15
16
17
18 Strain fitness (log2 ratio) -1
0
1 at 14.856 kb on + strand, within GFF3240 at 14.857 kb on - strand, within GFF3240 at 14.874 kb on + strand, within GFF3240 at 14.875 kb on - strand, within GFF3240 at 15.109 kb on - strand, within GFF3240 at 15.115 kb on + strand, within GFF3240 at 15.342 kb on + strand, within GFF3240 at 15.343 kb on - strand, within GFF3240 at 15.343 kb on - strand, within GFF3240 at 15.376 kb on + strand, within GFF3240 at 15.376 kb on + strand, within GFF3240 at 15.427 kb on + strand, within GFF3240 at 15.427 kb on + strand, within GFF3240 at 15.555 kb on + strand, within GFF3240 at 15.555 kb on + strand, within GFF3240 at 15.555 kb on + strand, within GFF3240 at 15.560 kb on + strand, within GFF3240 at 15.588 kb on + strand at 15.824 kb on + strand at 15.825 kb on - strand at 15.825 kb on - strand at 15.953 kb on - strand, within GFF3241 at 15.973 kb on - strand, within GFF3241 at 16.152 kb on - strand, within GFF3241 at 16.314 kb on + strand, within GFF3241 at 16.528 kb on + strand, within GFF3241 at 16.529 kb on - strand, within GFF3241 at 16.728 kb on + strand, within GFF3241 at 16.955 kb on + strand at 17.080 kb on - strand at 17.156 kb on + strand at 17.162 kb on + strand at 17.431 kb on + strand, within GFF3242 at 17.663 kb on - strand, within GFF3242 at 17.878 kb on + strand, within GFF3242 at 17.977 kb on + strand, within GFF3242 at 18.033 kb on + strand, within GFF3242
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.875 MOI remove 14,856 + GFF3240 0.52 +0.3 14,857 - GFF3240 0.52 -0.2 14,874 + GFF3240 0.53 +0.2 14,875 - GFF3240 0.53 -0.0 15,109 - GFF3240 0.65 +1.7 15,115 + GFF3240 0.65 +0.0 15,342 + GFF3240 0.77 -0.3 15,343 - GFF3240 0.77 -0.4 15,343 - GFF3240 0.77 +0.7 15,376 + GFF3240 0.79 -0.6 15,376 + GFF3240 0.79 -0.7 15,427 + GFF3240 0.82 -0.9 15,427 + GFF3240 0.82 +0.6 15,555 + GFF3240 0.88 -1.3 15,555 + GFF3240 0.88 +0.1 15,555 + GFF3240 0.88 -0.6 15,560 + GFF3240 0.89 -0.5 15,588 + +0.2 15,824 + +0.5 15,825 - +0.4 15,825 - -0.2 15,953 - GFF3241 0.13 -0.8 15,973 - GFF3241 0.14 -0.2 16,152 - GFF3241 0.29 -0.5 16,314 + GFF3241 0.42 +0.2 16,528 + GFF3241 0.59 -0.4 16,529 - GFF3241 0.59 +0.5 16,728 + GFF3241 0.75 -0.4 16,955 + -1.6 17,080 - +0.0 17,156 + -1.3 17,162 + +0.2 17,431 + GFF3242 0.23 +0.4 17,663 - GFF3242 0.37 -0.3 17,878 + GFF3242 0.50 +0.5 17,977 + GFF3242 0.56 -0.6 18,033 + GFF3242 0.60 +0.5
Or see this region's nucleotide sequence