Experiment: LB_plus_SM_buffer with EIP16_phage 0.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3204 and GFF3205 are separated by 25 nucleotides GFF3205 and GFF3206 are separated by 207 nucleotides
GFF3204 - Inner membrane protein, at 207,150 to 208,460
GFF3204
GFF3205 - Serine transporter, at 208,486 to 209,925
GFF3205
GFF3206 - L-serine dehydratase, beta subunit (EC 4.3.1.17) / L-serine dehydratase, alpha subunit (EC 4.3.1.17), at 210,133 to 211,497
GFF3206
Position (kb) 208
209
210 Strain fitness (log2 ratio) -2
-1
0
1
2 at 207.529 kb on + strand, within GFF3204 at 207.530 kb on - strand, within GFF3204 at 207.530 kb on - strand, within GFF3204 at 207.534 kb on - strand, within GFF3204 at 207.556 kb on - strand, within GFF3204 at 207.627 kb on - strand, within GFF3204 at 208.083 kb on - strand, within GFF3204 at 208.088 kb on + strand, within GFF3204 at 208.157 kb on + strand, within GFF3204 at 208.272 kb on - strand, within GFF3204 at 208.398 kb on + strand at 208.482 kb on + strand at 208.483 kb on - strand at 208.484 kb on + strand at 208.484 kb on + strand at 208.536 kb on + strand at 208.563 kb on - strand at 208.563 kb on - strand at 208.636 kb on - strand, within GFF3205 at 208.642 kb on - strand, within GFF3205 at 208.856 kb on - strand, within GFF3205 at 209.136 kb on + strand, within GFF3205 at 209.136 kb on + strand, within GFF3205 at 209.151 kb on + strand, within GFF3205 at 209.200 kb on - strand, within GFF3205 at 209.200 kb on - strand, within GFF3205 at 209.202 kb on - strand, within GFF3205 at 209.202 kb on - strand, within GFF3205 at 209.245 kb on + strand, within GFF3205 at 209.388 kb on + strand, within GFF3205 at 209.388 kb on + strand, within GFF3205 at 209.397 kb on - strand, within GFF3205 at 209.477 kb on - strand, within GFF3205 at 209.557 kb on - strand, within GFF3205 at 209.580 kb on - strand, within GFF3205 at 209.599 kb on - strand, within GFF3205 at 209.601 kb on + strand, within GFF3205 at 209.601 kb on + strand, within GFF3205 at 209.601 kb on + strand, within GFF3205 at 209.602 kb on - strand, within GFF3205 at 209.602 kb on - strand, within GFF3205 at 209.692 kb on - strand, within GFF3205 at 209.692 kb on - strand, within GFF3205 at 209.716 kb on - strand, within GFF3205 at 209.792 kb on - strand at 209.803 kb on - strand at 209.838 kb on - strand at 210.094 kb on - strand at 210.106 kb on - strand at 210.313 kb on - strand, within GFF3206 at 210.321 kb on + strand, within GFF3206 at 210.321 kb on + strand, within GFF3206 at 210.335 kb on - strand, within GFF3206 at 210.399 kb on + strand, within GFF3206 at 210.400 kb on - strand, within GFF3206 at 210.521 kb on + strand, within GFF3206 at 210.521 kb on + strand, within GFF3206 at 210.522 kb on - strand, within GFF3206 at 210.565 kb on - strand, within GFF3206 at 210.600 kb on - strand, within GFF3206 at 210.705 kb on + strand, within GFF3206
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.875 MOI remove 207,529 + GFF3204 0.29 -0.1 207,530 - GFF3204 0.29 -1.0 207,530 - GFF3204 0.29 +0.2 207,534 - GFF3204 0.29 +0.7 207,556 - GFF3204 0.31 -1.1 207,627 - GFF3204 0.36 -0.1 208,083 - GFF3204 0.71 +0.6 208,088 + GFF3204 0.72 +0.2 208,157 + GFF3204 0.77 -0.2 208,272 - GFF3204 0.86 +0.0 208,398 + +2.2 208,482 + -0.3 208,483 - -0.3 208,484 + +0.1 208,484 + -0.1 208,536 + +0.3 208,563 - -0.2 208,563 - -2.3 208,636 - GFF3205 0.10 -0.0 208,642 - GFF3205 0.11 +0.1 208,856 - GFF3205 0.26 +0.5 209,136 + GFF3205 0.45 -0.1 209,136 + GFF3205 0.45 +1.2 209,151 + GFF3205 0.46 -0.0 209,200 - GFF3205 0.50 +0.1 209,200 - GFF3205 0.50 -0.1 209,202 - GFF3205 0.50 +0.2 209,202 - GFF3205 0.50 +0.1 209,245 + GFF3205 0.53 -0.1 209,388 + GFF3205 0.63 -1.3 209,388 + GFF3205 0.63 -0.3 209,397 - GFF3205 0.63 +0.0 209,477 - GFF3205 0.69 +0.2 209,557 - GFF3205 0.74 +0.2 209,580 - GFF3205 0.76 +0.1 209,599 - GFF3205 0.77 +0.3 209,601 + GFF3205 0.77 +0.5 209,601 + GFF3205 0.77 -0.0 209,601 + GFF3205 0.77 -0.2 209,602 - GFF3205 0.78 -0.4 209,602 - GFF3205 0.78 +0.1 209,692 - GFF3205 0.84 +0.1 209,692 - GFF3205 0.84 +0.0 209,716 - GFF3205 0.85 -0.2 209,792 - +1.0 209,803 - +0.6 209,838 - +0.5 210,094 - +0.1 210,106 - -0.5 210,313 - GFF3206 0.13 -0.3 210,321 + GFF3206 0.14 +0.2 210,321 + GFF3206 0.14 +1.3 210,335 - GFF3206 0.15 -0.1 210,399 + GFF3206 0.19 -0.2 210,400 - GFF3206 0.20 +0.1 210,521 + GFF3206 0.28 +1.0 210,521 + GFF3206 0.28 +0.8 210,522 - GFF3206 0.28 -0.1 210,565 - GFF3206 0.32 +0.0 210,600 - GFF3206 0.34 +0.0 210,705 + GFF3206 0.42 -0.7
Or see this region's nucleotide sequence