Experiment: LB_plus_SM_buffer with EIP16_phage 0.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3055 and GFF3056 overlap by 4 nucleotides GFF3056 and GFF3057 are separated by 7 nucleotides GFF3057 and GFF3058 overlap by 8 nucleotides GFF3058 and GFF3059 are separated by 90 nucleotides
GFF3055 - Integral membrane protein YggT, involved in response to extracytoplasmic stress (osmotic shock), at 66,802 to 67,368
GFF3055
GFF3056 - COG1872, at 67,365 to 67,655
GFF3056
GFF3057 - Nucleoside 5-triphosphatase RdgB (dHAPTP, dITP, XTP-specific) (EC 3.6.1.15), at 67,663 to 68,256
GFF3057
GFF3058 - Radical SAM family enzyme, similar to coproporphyrinogen III oxidase, oxygen-independent, clustered with nucleoside-triphosphatase RdgB, at 68,249 to 69,385
GFF3058
GFF3059 - Putative alpha helix chain, at 69,476 to 70,483
GFF3059
Position (kb)
68
69
70 Strain fitness (log2 ratio)
-1
0
1
2
3 at 67.288 kb on + strand, within GFF3055 at 67.289 kb on - strand, within GFF3055 at 67.289 kb on - strand, within GFF3055 at 67.300 kb on - strand, within GFF3055 at 67.319 kb on + strand at 67.319 kb on + strand at 67.439 kb on + strand, within GFF3056 at 67.653 kb on + strand at 67.657 kb on + strand at 67.912 kb on + strand, within GFF3057 at 67.913 kb on - strand, within GFF3057 at 67.924 kb on + strand, within GFF3057 at 67.980 kb on - strand, within GFF3057 at 68.148 kb on - strand, within GFF3057 at 68.255 kb on - strand at 68.278 kb on + strand at 68.278 kb on + strand at 68.285 kb on - strand at 68.806 kb on + strand, within GFF3058 at 68.929 kb on - strand, within GFF3058 at 68.931 kb on + strand, within GFF3058 at 69.008 kb on + strand, within GFF3058 at 69.118 kb on + strand, within GFF3058 at 69.119 kb on - strand, within GFF3058 at 69.297 kb on - strand at 69.297 kb on - strand at 69.351 kb on + strand at 69.481 kb on + strand at 69.599 kb on + strand, within GFF3059 at 69.680 kb on + strand, within GFF3059 at 69.702 kb on - strand, within GFF3059 at 69.771 kb on - strand, within GFF3059 at 69.810 kb on + strand, within GFF3059 at 69.820 kb on + strand, within GFF3059 at 69.820 kb on + strand, within GFF3059 at 69.834 kb on - strand, within GFF3059 at 69.838 kb on - strand, within GFF3059 at 70.039 kb on + strand, within GFF3059 at 70.039 kb on + strand, within GFF3059 at 70.069 kb on + strand, within GFF3059 at 70.069 kb on + strand, within GFF3059 at 70.070 kb on - strand, within GFF3059 at 70.124 kb on + strand, within GFF3059 at 70.125 kb on - strand, within GFF3059 at 70.134 kb on + strand, within GFF3059 at 70.134 kb on + strand, within GFF3059 at 70.134 kb on + strand, within GFF3059 at 70.134 kb on + strand, within GFF3059 at 70.134 kb on + strand, within GFF3059 at 70.134 kb on + strand, within GFF3059 at 70.230 kb on + strand, within GFF3059 at 70.231 kb on - strand, within GFF3059 at 70.261 kb on - strand, within GFF3059 at 70.261 kb on - strand, within GFF3059 at 70.261 kb on - strand, within GFF3059 at 70.261 kb on - strand, within GFF3059 at 70.264 kb on + strand, within GFF3059 at 70.358 kb on + strand, within GFF3059 at 70.369 kb on - strand, within GFF3059 at 70.376 kb on - strand, within GFF3059 at 70.376 kb on - strand, within GFF3059
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.875 MOI remove 67,288 + GFF3055 0.86 -0.0 67,289 - GFF3055 0.86 +0.7 67,289 - GFF3055 0.86 +0.9 67,300 - GFF3055 0.88 +0.7 67,319 + +0.3 67,319 + -0.1 67,439 + GFF3056 0.25 -0.1 67,653 + -0.7 67,657 + +0.1 67,912 + GFF3057 0.42 +0.3 67,913 - GFF3057 0.42 -0.4 67,924 + GFF3057 0.44 -0.7 67,980 - GFF3057 0.53 +0.9 68,148 - GFF3057 0.82 +0.5 68,255 - +1.0 68,278 + +0.6 68,278 + +0.5 68,285 - +0.4 68,806 + GFF3058 0.49 +0.3 68,929 - GFF3058 0.60 +0.3 68,931 + GFF3058 0.60 +2.8 69,008 + GFF3058 0.67 +0.4 69,118 + GFF3058 0.76 +0.5 69,119 - GFF3058 0.77 +0.6 69,297 - +0.9 69,297 - +0.8 69,351 + -1.1 69,481 + -0.6 69,599 + GFF3059 0.12 -0.6 69,680 + GFF3059 0.20 +0.7 69,702 - GFF3059 0.22 +0.9 69,771 - GFF3059 0.29 -0.1 69,810 + GFF3059 0.33 +0.2 69,820 + GFF3059 0.34 +0.2 69,820 + GFF3059 0.34 +0.3 69,834 - GFF3059 0.36 +2.4 69,838 - GFF3059 0.36 -0.1 70,039 + GFF3059 0.56 -0.3 70,039 + GFF3059 0.56 -0.4 70,069 + GFF3059 0.59 -0.4 70,069 + GFF3059 0.59 +0.4 70,070 - GFF3059 0.59 -0.0 70,124 + GFF3059 0.64 +0.4 70,125 - GFF3059 0.64 +0.5 70,134 + GFF3059 0.65 +1.0 70,134 + GFF3059 0.65 +0.6 70,134 + GFF3059 0.65 +2.2 70,134 + GFF3059 0.65 +0.1 70,134 + GFF3059 0.65 -0.2 70,134 + GFF3059 0.65 -0.9 70,230 + GFF3059 0.75 +0.2 70,231 - GFF3059 0.75 -1.0 70,261 - GFF3059 0.78 -0.2 70,261 - GFF3059 0.78 +0.8 70,261 - GFF3059 0.78 +0.7 70,261 - GFF3059 0.78 +0.4 70,264 + GFF3059 0.78 -0.5 70,358 + GFF3059 0.88 -0.3 70,369 - GFF3059 0.89 -0.4 70,376 - GFF3059 0.89 +0.5 70,376 - GFF3059 0.89 +0.2
Or see this region's nucleotide sequence