Experiment: LB_plus_SM_buffer with EIP16_phage 0.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF281 and GFF282 are separated by 1 nucleotides GFF282 and GFF283 overlap by 2 nucleotides GFF283 and GFF284 are separated by 257 nucleotides GFF284 and GFF285 are separated by 109 nucleotides
GFF281 - Uncharacterized protein YidR, at 879 to 2,105
GFF281
GFF282 - Outer membrane lipoprotein YidQ, at 2,107 to 2,367
GFF282
GFF283 - hypothetical protein, at 2,366 to 2,485
GFF283
GFF284 - 16 kDa heat shock protein A, at 2,743 to 3,162
GFF284
GFF285 - 16 kDa heat shock protein B, at 3,272 to 3,700
GFF285
Position (kb)
2
3 Strain fitness (log2 ratio)
-1
0
1
2 at 1.112 kb on + strand, within GFF281 at 1.200 kb on - strand, within GFF281 at 1.220 kb on + strand, within GFF281 at 1.221 kb on - strand, within GFF281 at 1.301 kb on + strand, within GFF281 at 1.301 kb on + strand, within GFF281 at 1.305 kb on + strand, within GFF281 at 1.307 kb on + strand, within GFF281 at 1.421 kb on + strand, within GFF281 at 1.422 kb on - strand, within GFF281 at 1.485 kb on - strand, within GFF281 at 1.591 kb on + strand, within GFF281 at 1.694 kb on + strand, within GFF281 at 1.700 kb on + strand, within GFF281 at 1.758 kb on - strand, within GFF281 at 1.856 kb on - strand, within GFF281 at 1.876 kb on + strand, within GFF281 at 1.890 kb on - strand, within GFF281 at 2.080 kb on + strand at 2.081 kb on - strand at 2.104 kb on - strand at 2.232 kb on + strand, within GFF282 at 2.232 kb on + strand, within GFF282 at 2.336 kb on + strand, within GFF282 at 2.336 kb on + strand, within GFF282 at 2.341 kb on - strand, within GFF282 at 2.341 kb on - strand, within GFF282 at 2.341 kb on - strand, within GFF282 at 2.390 kb on - strand, within GFF283 at 2.390 kb on - strand, within GFF283 at 2.410 kb on + strand, within GFF283 at 2.481 kb on + strand at 2.484 kb on - strand at 2.613 kb on + strand at 2.624 kb on + strand at 2.628 kb on + strand at 2.642 kb on + strand at 2.644 kb on + strand at 2.651 kb on + strand at 2.652 kb on - strand at 2.742 kb on + strand at 2.823 kb on + strand, within GFF284 at 2.887 kb on + strand, within GFF284 at 2.997 kb on + strand, within GFF284 at 2.998 kb on - strand, within GFF284 at 2.998 kb on - strand, within GFF284 at 3.001 kb on + strand, within GFF284 at 3.001 kb on + strand, within GFF284 at 3.002 kb on - strand, within GFF284 at 3.039 kb on + strand, within GFF284 at 3.039 kb on + strand, within GFF284 at 3.100 kb on + strand, within GFF284 at 3.100 kb on + strand, within GFF284 at 3.101 kb on - strand, within GFF284 at 3.102 kb on + strand, within GFF284 at 3.255 kb on + strand at 3.256 kb on - strand at 3.271 kb on + strand at 3.278 kb on - strand at 3.281 kb on + strand at 3.282 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.875 MOI remove 1,112 + GFF281 0.19 -0.1 1,200 - GFF281 0.26 -0.4 1,220 + GFF281 0.28 -0.4 1,221 - GFF281 0.28 +0.9 1,301 + GFF281 0.34 -0.3 1,301 + GFF281 0.34 +0.2 1,305 + GFF281 0.35 +1.7 1,307 + GFF281 0.35 +0.7 1,421 + GFF281 0.44 -0.6 1,422 - GFF281 0.44 -0.4 1,485 - GFF281 0.49 -1.0 1,591 + GFF281 0.58 -0.4 1,694 + GFF281 0.66 +0.8 1,700 + GFF281 0.67 +0.5 1,758 - GFF281 0.72 -0.5 1,856 - GFF281 0.80 -0.3 1,876 + GFF281 0.81 +0.6 1,890 - GFF281 0.82 -0.0 2,080 + +0.2 2,081 - +0.4 2,104 - +1.3 2,232 + GFF282 0.48 +0.3 2,232 + GFF282 0.48 +1.1 2,336 + GFF282 0.88 +2.1 2,336 + GFF282 0.88 -0.3 2,341 - GFF282 0.90 -0.1 2,341 - GFF282 0.90 -0.4 2,341 - GFF282 0.90 +0.3 2,390 - GFF283 0.20 -0.4 2,390 - GFF283 0.20 +0.1 2,410 + GFF283 0.37 +0.1 2,481 + -0.0 2,484 - +0.7 2,613 + -0.3 2,624 + +1.1 2,628 + +0.2 2,642 + -0.1 2,644 + -0.3 2,651 + -0.9 2,652 - -1.3 2,742 + +0.1 2,823 + GFF284 0.19 +0.9 2,887 + GFF284 0.34 +1.0 2,997 + GFF284 0.60 +0.6 2,998 - GFF284 0.61 -0.0 2,998 - GFF284 0.61 -0.6 3,001 + GFF284 0.61 +0.3 3,001 + GFF284 0.61 +0.3 3,002 - GFF284 0.62 -0.1 3,039 + GFF284 0.70 +0.1 3,039 + GFF284 0.70 +0.0 3,100 + GFF284 0.85 -0.8 3,100 + GFF284 0.85 +0.1 3,101 - GFF284 0.85 +0.4 3,102 + GFF284 0.85 -0.3 3,255 + +0.3 3,256 - -0.3 3,271 + -0.5 3,278 - -0.9 3,281 + +0.4 3,282 - -0.1
Or see this region's nucleotide sequence