Experiment: LB_plus_SM_buffer with EIP16_phage 0.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2581 and GFF2582 overlap by 1 nucleotides GFF2582 and GFF2583 are separated by 4 nucleotides GFF2583 and GFF2584 are separated by 223 nucleotides GFF2584 and GFF2585 are separated by 4 nucleotides
GFF2581 - Putative acetyltransferase, at 103,963 to 104,478
GFF2581
GFF2582 - Putative inner membrane protein, at 104,478 to 104,927
GFF2582
GFF2583 - FIG00638150: hypothetical protein, at 104,932 to 105,165
GFF2583
GFF2584 - Putative virulence effector protein, at 105,389 to 106,708
GFF2584
GFF2585 - SrfB, at 106,713 to 109,694
GFF2585
Position (kb)
105
106
107 Strain fitness (log2 ratio)
-1
0
1 at 104.544 kb on - strand, within GFF2582 at 104.546 kb on + strand, within GFF2582 at 104.556 kb on - strand, within GFF2582 at 104.588 kb on + strand, within GFF2582 at 104.620 kb on + strand, within GFF2582 at 104.642 kb on + strand, within GFF2582 at 104.643 kb on - strand, within GFF2582 at 104.926 kb on - strand at 105.041 kb on + strand, within GFF2583 at 105.166 kb on - strand at 105.210 kb on - strand at 105.210 kb on - strand at 105.211 kb on + strand at 105.211 kb on + strand at 105.266 kb on - strand at 105.292 kb on + strand at 105.293 kb on - strand at 105.428 kb on + strand at 105.720 kb on + strand, within GFF2584 at 106.080 kb on + strand, within GFF2584 at 106.096 kb on - strand, within GFF2584 at 106.096 kb on - strand, within GFF2584 at 106.348 kb on + strand, within GFF2584 at 106.483 kb on - strand, within GFF2584 at 106.483 kb on - strand, within GFF2584 at 106.485 kb on + strand, within GFF2584 at 106.485 kb on + strand, within GFF2584 at 106.486 kb on - strand, within GFF2584 at 106.486 kb on - strand, within GFF2584 at 106.613 kb on - strand at 106.712 kb on + strand at 106.734 kb on + strand at 107.043 kb on - strand, within GFF2585 at 107.066 kb on + strand, within GFF2585 at 107.141 kb on + strand, within GFF2585 at 107.420 kb on + strand, within GFF2585 at 107.420 kb on + strand, within GFF2585 at 107.420 kb on + strand, within GFF2585 at 107.421 kb on - strand, within GFF2585 at 107.421 kb on - strand, within GFF2585 at 107.477 kb on + strand, within GFF2585 at 107.592 kb on - strand, within GFF2585
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.875 MOI remove 104,544 - GFF2582 0.15 +0.1 104,546 + GFF2582 0.15 +0.4 104,556 - GFF2582 0.17 -0.3 104,588 + GFF2582 0.24 +0.0 104,620 + GFF2582 0.32 +0.5 104,642 + GFF2582 0.36 +1.2 104,643 - GFF2582 0.37 +0.3 104,926 - +0.0 105,041 + GFF2583 0.47 +0.2 105,166 - +0.6 105,210 - +0.1 105,210 - -0.4 105,211 + +0.8 105,211 + -0.1 105,266 - -0.4 105,292 + -0.3 105,293 - -0.2 105,428 + +0.8 105,720 + GFF2584 0.25 +0.4 106,080 + GFF2584 0.52 +0.2 106,096 - GFF2584 0.54 +0.5 106,096 - GFF2584 0.54 +0.6 106,348 + GFF2584 0.73 +0.5 106,483 - GFF2584 0.83 +1.2 106,483 - GFF2584 0.83 +1.0 106,485 + GFF2584 0.83 +0.4 106,485 + GFF2584 0.83 +0.5 106,486 - GFF2584 0.83 +0.6 106,486 - GFF2584 0.83 +1.0 106,613 - +0.7 106,712 + +0.7 106,734 + +0.5 107,043 - GFF2585 0.11 +0.2 107,066 + GFF2585 0.12 +0.5 107,141 + GFF2585 0.14 +1.2 107,420 + GFF2585 0.24 +0.6 107,420 + GFF2585 0.24 +0.0 107,420 + GFF2585 0.24 +0.4 107,421 - GFF2585 0.24 +0.1 107,421 - GFF2585 0.24 +0.7 107,477 + GFF2585 0.26 +1.4 107,592 - GFF2585 0.29 +0.8
Or see this region's nucleotide sequence