Experiment: LB_plus_SM_buffer with EIP16_phage 0.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2321 and GFF2322 are separated by 410 nucleotides GFF2322 and GFF2323 are separated by 264 nucleotides GFF2323 and GFF2324 are separated by 2 nucleotides
GFF2321 - FIG01045707: hypothetical protein, at 107,483 to 107,644
GFF2321
GFF2322 - Methionine repressor MetJ, at 108,055 to 108,372
GFF2322
GFF2323 - Cystathionine gamma-synthase (EC 2.5.1.48), at 108,637 to 109,797
GFF2323
GFF2324 - Aspartokinase (EC 2.7.2.4) / Homoserine dehydrogenase (EC 1.1.1.3), at 109,800 to 112,232
GFF2324
Position (kb)
108
109
110 Strain fitness (log2 ratio)
-1
0
1
2 at 108.324 kb on + strand, within GFF2322 at 108.382 kb on - strand at 108.400 kb on + strand at 108.444 kb on + strand at 108.449 kb on + strand at 108.504 kb on - strand at 108.536 kb on + strand at 108.537 kb on - strand at 108.584 kb on + strand at 108.636 kb on + strand at 108.636 kb on + strand at 108.636 kb on + strand at 108.694 kb on + strand at 108.694 kb on + strand at 108.730 kb on - strand at 108.733 kb on + strand at 108.734 kb on - strand at 108.735 kb on + strand at 108.848 kb on + strand, within GFF2323 at 108.949 kb on + strand, within GFF2323 at 109.155 kb on + strand, within GFF2323 at 109.275 kb on + strand, within GFF2323 at 109.347 kb on + strand, within GFF2323 at 109.348 kb on - strand, within GFF2323 at 109.351 kb on + strand, within GFF2323 at 109.352 kb on - strand, within GFF2323 at 109.352 kb on - strand, within GFF2323 at 109.390 kb on - strand, within GFF2323 at 109.720 kb on - strand at 109.752 kb on - strand at 109.859 kb on + strand at 109.955 kb on + strand at 109.973 kb on + strand at 110.023 kb on + strand at 110.092 kb on + strand, within GFF2324 at 110.093 kb on - strand, within GFF2324 at 110.105 kb on + strand, within GFF2324 at 110.164 kb on - strand, within GFF2324 at 110.311 kb on + strand, within GFF2324 at 110.336 kb on - strand, within GFF2324 at 110.414 kb on - strand, within GFF2324 at 110.506 kb on - strand, within GFF2324 at 110.661 kb on - strand, within GFF2324 at 110.661 kb on - strand, within GFF2324 at 110.664 kb on + strand, within GFF2324 at 110.747 kb on + strand, within GFF2324 at 110.747 kb on + strand, within GFF2324 at 110.748 kb on - strand, within GFF2324 at 110.748 kb on - strand, within GFF2324
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.875 MOI remove 108,324 + GFF2322 0.85 +1.2 108,382 - +0.9 108,400 + +1.7 108,444 + +1.6 108,449 + +1.5 108,504 - +0.2 108,536 + -0.1 108,537 - -0.5 108,584 + +0.3 108,636 + -0.4 108,636 + -0.1 108,636 + +0.5 108,694 + -0.4 108,694 + +0.1 108,730 - +0.3 108,733 + +0.1 108,734 - -0.5 108,735 + +0.6 108,848 + GFF2323 0.18 +0.1 108,949 + GFF2323 0.27 +0.9 109,155 + GFF2323 0.45 +0.5 109,275 + GFF2323 0.55 +0.3 109,347 + GFF2323 0.61 +0.6 109,348 - GFF2323 0.61 +1.0 109,351 + GFF2323 0.61 +0.1 109,352 - GFF2323 0.62 +0.8 109,352 - GFF2323 0.62 +0.7 109,390 - GFF2323 0.65 +0.3 109,720 - +1.2 109,752 - +1.7 109,859 + +1.3 109,955 + +0.9 109,973 + +0.6 110,023 + +0.6 110,092 + GFF2324 0.12 +1.2 110,093 - GFF2324 0.12 +1.3 110,105 + GFF2324 0.13 +1.6 110,164 - GFF2324 0.15 +1.5 110,311 + GFF2324 0.21 +2.0 110,336 - GFF2324 0.22 +1.2 110,414 - GFF2324 0.25 +1.3 110,506 - GFF2324 0.29 +1.4 110,661 - GFF2324 0.35 +1.2 110,661 - GFF2324 0.35 +1.4 110,664 + GFF2324 0.36 +2.5 110,747 + GFF2324 0.39 +1.3 110,747 + GFF2324 0.39 +1.3 110,748 - GFF2324 0.39 +1.4 110,748 - GFF2324 0.39 +2.3
Or see this region's nucleotide sequence