Experiment: LB_plus_SM_buffer with EIP16_phage 0.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1811 and GFF1812 are separated by 74 nucleotides GFF1812 and GFF1813 are separated by 630 nucleotides GFF1813 and GFF1814 are separated by 55 nucleotides
GFF1811 - Adenosine deaminase (EC 3.5.4.4), at 35,288 to 36,289
GFF1811
GFF1812 - Uncharacterized oxidoreductase ydgJ (EC 1.-.-.-), at 36,364 to 37,404
GFF1812
GFF1813 - Cnu protein, at 38,035 to 38,250
GFF1813
GFF1814 - Putative inner membrane protein, at 38,306 to 38,779
GFF1814
Position (kb) 36
37
38 Strain fitness (log2 ratio) -1
0
1
2 at 35.713 kb on + strand, within GFF1811 at 35.737 kb on - strand, within GFF1811 at 35.737 kb on - strand, within GFF1811 at 35.867 kb on - strand, within GFF1811 at 35.955 kb on + strand, within GFF1811 at 36.142 kb on - strand, within GFF1811 at 36.298 kb on + strand at 36.338 kb on + strand at 36.497 kb on + strand, within GFF1812 at 36.497 kb on + strand, within GFF1812 at 36.500 kb on + strand, within GFF1812 at 36.510 kb on - strand, within GFF1812 at 36.693 kb on - strand, within GFF1812 at 37.229 kb on - strand, within GFF1812 at 37.357 kb on - strand at 37.423 kb on - strand at 37.482 kb on + strand at 37.483 kb on - strand at 37.569 kb on - strand at 37.581 kb on + strand at 37.686 kb on + strand at 37.696 kb on + strand at 37.747 kb on - strand at 37.751 kb on + strand at 37.831 kb on + strand at 37.831 kb on + strand at 37.831 kb on + strand at 37.919 kb on - strand at 38.149 kb on - strand, within GFF1813 at 38.153 kb on - strand, within GFF1813 at 38.285 kb on - strand at 38.338 kb on + strand at 38.345 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.875 MOI remove 35,713 + GFF1811 0.42 +0.1 35,737 - GFF1811 0.45 +0.6 35,737 - GFF1811 0.45 +0.5 35,867 - GFF1811 0.58 +0.6 35,955 + GFF1811 0.67 +0.7 36,142 - GFF1811 0.85 +1.1 36,298 + +0.2 36,338 + +0.5 36,497 + GFF1812 0.13 +0.4 36,497 + GFF1812 0.13 +0.1 36,500 + GFF1812 0.13 +0.7 36,510 - GFF1812 0.14 +0.2 36,693 - GFF1812 0.32 +0.3 37,229 - GFF1812 0.83 +0.2 37,357 - -0.4 37,423 - +0.6 37,482 + -0.1 37,483 - -0.1 37,569 - +2.2 37,581 + -0.1 37,686 + +1.4 37,696 + -1.4 37,747 - +0.4 37,751 + +0.2 37,831 + -0.0 37,831 + +0.5 37,831 + +0.4 37,919 - +1.4 38,149 - GFF1813 0.53 +0.8 38,153 - GFF1813 0.55 +0.6 38,285 - +0.3 38,338 + -0.3 38,345 - +0.5
Or see this region's nucleotide sequence