Experiment: LB_plus_SM_buffer with EIP16_phage 0.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1791 and GFF1792 overlap by 14 nucleotides GFF1792 and GFF1793 are separated by 111 nucleotides GFF1793 and GFF1794 are separated by 168 nucleotides GFF1794 and GFF1795 are separated by 174 nucleotides
GFF1791 - Spermidine export protein MdtI, at 12,855 to 13,184
GFF1791
GFF1792 - Spermidine export protein MdtJ, at 13,171 to 13,533
GFF1792
GFF1793 - FIG01046102: hypothetical protein, at 13,645 to 13,782
GFF1793
GFF1794 - Putative transport protein, at 13,951 to 14,985
GFF1794
GFF1795 - NAD(P) transhydrogenase subunit beta (EC 1.6.1.2), at 15,160 to 16,548
GFF1795
Position (kb) 13
14
15 Strain fitness (log2 ratio) -1
0
1 at 13.017 kb on + strand, within GFF1791 at 13.018 kb on - strand, within GFF1791 at 13.066 kb on - strand, within GFF1791 at 13.082 kb on + strand, within GFF1791 at 13.083 kb on - strand, within GFF1791 at 13.164 kb on + strand at 13.237 kb on + strand, within GFF1792 at 13.401 kb on + strand, within GFF1792 at 13.656 kb on + strand at 13.996 kb on - strand at 14.271 kb on + strand, within GFF1794 at 14.329 kb on + strand, within GFF1794 at 14.408 kb on + strand, within GFF1794 at 14.426 kb on + strand, within GFF1794 at 14.430 kb on + strand, within GFF1794 at 14.430 kb on + strand, within GFF1794 at 14.431 kb on - strand, within GFF1794 at 14.496 kb on + strand, within GFF1794 at 14.634 kb on - strand, within GFF1794 at 14.653 kb on + strand, within GFF1794 at 14.653 kb on + strand, within GFF1794 at 14.829 kb on - strand, within GFF1794 at 14.867 kb on + strand, within GFF1794 at 14.868 kb on - strand, within GFF1794 at 14.993 kb on + strand at 14.997 kb on + strand at 14.997 kb on + strand at 14.997 kb on + strand at 15.053 kb on + strand at 15.154 kb on - strand at 15.486 kb on + strand, within GFF1795 at 15.619 kb on - strand, within GFF1795 at 15.775 kb on - strand, within GFF1795 at 15.775 kb on - strand, within GFF1795 at 15.779 kb on + strand, within GFF1795 at 15.780 kb on - strand, within GFF1795 at 15.780 kb on - strand, within GFF1795
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.875 MOI remove 13,017 + GFF1791 0.49 -0.7 13,018 - GFF1791 0.49 +0.2 13,066 - GFF1791 0.64 +0.1 13,082 + GFF1791 0.69 -0.5 13,083 - GFF1791 0.69 +0.1 13,164 + -0.7 13,237 + GFF1792 0.18 +1.3 13,401 + GFF1792 0.63 +0.3 13,656 + +0.2 13,996 - -0.4 14,271 + GFF1794 0.31 -0.3 14,329 + GFF1794 0.37 -0.3 14,408 + GFF1794 0.44 +0.2 14,426 + GFF1794 0.46 +0.1 14,430 + GFF1794 0.46 -0.5 14,430 + GFF1794 0.46 -0.9 14,431 - GFF1794 0.46 +0.3 14,496 + GFF1794 0.53 -0.5 14,634 - GFF1794 0.66 -0.2 14,653 + GFF1794 0.68 +0.1 14,653 + GFF1794 0.68 +0.0 14,829 - GFF1794 0.85 -0.8 14,867 + GFF1794 0.89 +0.1 14,868 - GFF1794 0.89 -0.7 14,993 + +0.4 14,997 + -0.3 14,997 + +0.2 14,997 + +0.4 15,053 + +0.2 15,154 - -0.6 15,486 + GFF1795 0.23 -0.8 15,619 - GFF1795 0.33 +0.1 15,775 - GFF1795 0.44 +0.6 15,775 - GFF1795 0.44 -0.1 15,779 + GFF1795 0.45 +0.7 15,780 - GFF1795 0.45 +0.2 15,780 - GFF1795 0.45 -0.8
Or see this region's nucleotide sequence