Experiment: LB_plus_SM_buffer with EIP16_phage 0.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1710 and GFF1711 are separated by 347 nucleotides GFF1711 and GFF1712 overlap by 7 nucleotides
GFF1710 - Kup system potassium uptake protein, at 8,377 to 10,245
GFF1710
GFF1711 - Putative regulator protein, at 10,593 to 12,089
GFF1711
GFF1712 - FIG00637882: hypothetical protein, at 12,083 to 13,534
GFF1712
Position (kb)
10
11
12
13 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 9.601 kb on - strand, within GFF1710 at 9.616 kb on - strand, within GFF1710 at 9.661 kb on + strand, within GFF1710 at 9.662 kb on - strand, within GFF1710 at 9.684 kb on + strand, within GFF1710 at 9.751 kb on + strand, within GFF1710 at 9.752 kb on - strand, within GFF1710 at 9.937 kb on - strand, within GFF1710 at 9.940 kb on + strand, within GFF1710 at 9.941 kb on - strand, within GFF1710 at 9.966 kb on + strand, within GFF1710 at 9.967 kb on - strand, within GFF1710 at 10.043 kb on + strand, within GFF1710 at 10.156 kb on - strand at 10.157 kb on + strand at 10.158 kb on - strand at 10.198 kb on - strand at 10.198 kb on - strand at 10.262 kb on + strand at 10.264 kb on + strand at 10.386 kb on - strand at 10.387 kb on + strand at 10.388 kb on - strand at 10.388 kb on - strand at 10.388 kb on - strand at 10.388 kb on - strand at 10.388 kb on - strand at 10.391 kb on + strand at 10.392 kb on - strand at 10.406 kb on + strand at 10.446 kb on + strand at 10.481 kb on + strand at 10.521 kb on + strand at 10.544 kb on + strand at 10.545 kb on - strand at 10.556 kb on + strand at 10.557 kb on - strand at 10.667 kb on + strand at 10.668 kb on - strand at 10.668 kb on - strand at 10.690 kb on + strand at 10.958 kb on + strand, within GFF1711 at 11.226 kb on + strand, within GFF1711 at 11.291 kb on + strand, within GFF1711 at 11.292 kb on - strand, within GFF1711 at 11.313 kb on - strand, within GFF1711 at 11.332 kb on - strand, within GFF1711 at 11.424 kb on - strand, within GFF1711 at 11.588 kb on + strand, within GFF1711 at 11.621 kb on + strand, within GFF1711 at 11.622 kb on - strand, within GFF1711 at 11.643 kb on + strand, within GFF1711 at 11.644 kb on - strand, within GFF1711 at 11.684 kb on - strand, within GFF1711 at 11.715 kb on - strand, within GFF1711 at 11.732 kb on + strand, within GFF1711 at 11.873 kb on - strand, within GFF1711 at 11.880 kb on - strand, within GFF1711 at 11.915 kb on + strand, within GFF1711 at 11.976 kb on - strand at 12.055 kb on + strand at 12.056 kb on - strand at 12.056 kb on - strand at 12.106 kb on + strand at 12.106 kb on + strand at 12.107 kb on - strand at 12.311 kb on + strand, within GFF1712 at 12.399 kb on - strand, within GFF1712 at 12.436 kb on + strand, within GFF1712 at 12.511 kb on - strand, within GFF1712 at 12.522 kb on + strand, within GFF1712 at 12.523 kb on - strand, within GFF1712 at 12.613 kb on + strand, within GFF1712 at 12.638 kb on - strand, within GFF1712 at 12.655 kb on + strand, within GFF1712 at 12.669 kb on - strand, within GFF1712 at 12.718 kb on - strand, within GFF1712 at 12.862 kb on + strand, within GFF1712 at 12.863 kb on - strand, within GFF1712 at 13.049 kb on - strand, within GFF1712 at 13.049 kb on - strand, within GFF1712 at 13.063 kb on + strand, within GFF1712 at 13.087 kb on + strand, within GFF1712 at 13.087 kb on + strand, within GFF1712
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.875 MOI remove 9,601 - GFF1710 0.65 +0.2 9,616 - GFF1710 0.66 +1.1 9,661 + GFF1710 0.69 -0.1 9,662 - GFF1710 0.69 +0.8 9,684 + GFF1710 0.70 +0.6 9,751 + GFF1710 0.74 +1.1 9,752 - GFF1710 0.74 +0.7 9,937 - GFF1710 0.83 +0.8 9,940 + GFF1710 0.84 -0.3 9,941 - GFF1710 0.84 +0.8 9,966 + GFF1710 0.85 +0.3 9,967 - GFF1710 0.85 +1.1 10,043 + GFF1710 0.89 +0.3 10,156 - -1.4 10,157 + +1.1 10,158 - +1.8 10,198 - +0.6 10,198 - +0.4 10,262 + +0.6 10,264 + +0.7 10,386 - +0.6 10,387 + -0.8 10,388 - +0.4 10,388 - +0.5 10,388 - +1.1 10,388 - +0.7 10,388 - +0.3 10,391 + +0.2 10,392 - +0.5 10,406 + +0.5 10,446 + +0.5 10,481 + -0.3 10,521 + -0.5 10,544 + -1.0 10,545 - -0.2 10,556 + +0.9 10,557 - -0.2 10,667 + +0.2 10,668 - -0.3 10,668 - +0.0 10,690 + +0.6 10,958 + GFF1711 0.24 -0.1 11,226 + GFF1711 0.42 +0.5 11,291 + GFF1711 0.47 +0.3 11,292 - GFF1711 0.47 -0.4 11,313 - GFF1711 0.48 +0.1 11,332 - GFF1711 0.49 +0.6 11,424 - GFF1711 0.56 +0.6 11,588 + GFF1711 0.66 -0.0 11,621 + GFF1711 0.69 +1.5 11,622 - GFF1711 0.69 -2.2 11,643 + GFF1711 0.70 +1.7 11,644 - GFF1711 0.70 +0.1 11,684 - GFF1711 0.73 -0.2 11,715 - GFF1711 0.75 +0.2 11,732 + GFF1711 0.76 +0.0 11,873 - GFF1711 0.86 -0.4 11,880 - GFF1711 0.86 +0.4 11,915 + GFF1711 0.88 +0.5 11,976 - +0.9 12,055 + +0.1 12,056 - +0.6 12,056 - +0.4 12,106 + +0.5 12,106 + -0.3 12,107 - +0.1 12,311 + GFF1712 0.16 +0.0 12,399 - GFF1712 0.22 -0.0 12,436 + GFF1712 0.24 +0.2 12,511 - GFF1712 0.29 +0.8 12,522 + GFF1712 0.30 +0.1 12,523 - GFF1712 0.30 +0.2 12,613 + GFF1712 0.37 -0.1 12,638 - GFF1712 0.38 -0.4 12,655 + GFF1712 0.39 +0.8 12,669 - GFF1712 0.40 -0.3 12,718 - GFF1712 0.44 -0.1 12,862 + GFF1712 0.54 -0.1 12,863 - GFF1712 0.54 +1.0 13,049 - GFF1712 0.67 -0.1 13,049 - GFF1712 0.67 +0.8 13,063 + GFF1712 0.67 -0.3 13,087 + GFF1712 0.69 +0.8 13,087 + GFF1712 0.69 +0.1
Or see this region's nucleotide sequence