Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF1659

Experiment: LB_plus_SM_buffer with EIP16_phage 0.875 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1658 and GFF1659 are separated by 236 nucleotidesGFF1659 and GFF1660 overlap by 1 nucleotides GFF1658 - Rhamnogalacturonides degradation protein RhiN, at 42,865 to 44,004 GFF1658 GFF1659 - Flagellar protein FlhE, at 44,241 to 44,633 GFF1659 GFF1660 - Flagellar biosynthesis protein FlhA, at 44,633 to 46,711 GFF1660 Position (kb) 44 45Strain fitness (log2 ratio) -3 -2 -1 0 1at 43.342 kb on - strand, within GFF1658at 43.425 kb on - strand, within GFF1658at 43.425 kb on - strand, within GFF1658at 43.465 kb on - strand, within GFF1658at 43.781 kb on + strand, within GFF1658at 43.781 kb on + strand, within GFF1658at 43.781 kb on + strand, within GFF1658at 43.782 kb on - strand, within GFF1658at 44.129 kb on + strandat 44.129 kb on + strandat 44.292 kb on - strand, within GFF1659at 44.542 kb on - strand, within GFF1659at 44.547 kb on + strand, within GFF1659at 44.547 kb on + strand, within GFF1659at 44.548 kb on - strand, within GFF1659at 44.742 kb on - strandat 44.967 kb on - strand, within GFF1660at 44.978 kb on + strand, within GFF1660at 45.080 kb on + strand, within GFF1660

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.875 MOI
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43,342 - GFF1658 0.42 +0.3
43,425 - GFF1658 0.49 +0.6
43,425 - GFF1658 0.49 -0.9
43,465 - GFF1658 0.53 +1.2
43,781 + GFF1658 0.80 -0.1
43,781 + GFF1658 0.80 -3.3
43,781 + GFF1658 0.80 +1.3
43,782 - GFF1658 0.80 -0.3
44,129 + -0.1
44,129 + -0.2
44,292 - GFF1659 0.13 +0.1
44,542 - GFF1659 0.77 -0.3
44,547 + GFF1659 0.78 +0.0
44,547 + GFF1659 0.78 +0.5
44,548 - GFF1659 0.78 -0.1
44,742 - +0.9
44,967 - GFF1660 0.16 +1.5
44,978 + GFF1660 0.17 +1.0
45,080 + GFF1660 0.22 +1.4

Or see this region's nucleotide sequence