Experiment: LB_plus_SM_buffer with EIP16_phage 0.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1511 and GFF1512 overlap by 7 nucleotides GFF1512 and GFF1513 overlap by 14 nucleotides GFF1513 and GFF1514 overlap by 8 nucleotides GFF1514 and GFF1515 overlap by 21 nucleotides GFF1515 and GFF1516 are separated by 130 nucleotides
GFF1511 - FIG138517: Putative lipid carrier protein, at 18,502 to 19,026
GFF1511
GFF1512 - FIG002208: Acetyltransferase (EC 2.3.1.-), at 19,020 to 19,523
GFF1512
GFF1513 - FIG137864: putative endonuclease containing a URI domain, at 19,510 to 19,872
GFF1513
GFF1514 - FIG01219785: hypothetical protein, at 19,865 to 20,308
GFF1514
GFF1515 - General stress protein 18, at 20,288 to 20,806
GFF1515
GFF1516 - Nucleoside-diphosphate-sugar epimerases, at 20,937 to 21,572
GFF1516
Position (kb) 19
20
21 Strain fitness (log2 ratio) -2
-1
0
1
2 at 18.880 kb on + strand, within GFF1511 at 18.880 kb on + strand, within GFF1511 at 18.881 kb on - strand, within GFF1511 at 19.024 kb on + strand at 19.024 kb on + strand at 19.058 kb on + strand at 19.225 kb on + strand, within GFF1512 at 19.295 kb on - strand, within GFF1512 at 19.295 kb on - strand, within GFF1512 at 19.295 kb on - strand, within GFF1512 at 19.299 kb on + strand, within GFF1512 at 19.299 kb on + strand, within GFF1512 at 19.366 kb on + strand, within GFF1512 at 19.368 kb on + strand, within GFF1512 at 19.369 kb on - strand, within GFF1512 at 19.369 kb on - strand, within GFF1512 at 19.370 kb on + strand, within GFF1512 at 19.522 kb on - strand at 19.620 kb on - strand, within GFF1513 at 19.624 kb on + strand, within GFF1513 at 19.782 kb on + strand, within GFF1513 at 19.799 kb on + strand, within GFF1513 at 19.800 kb on - strand, within GFF1513 at 19.861 kb on - strand at 19.861 kb on - strand at 19.876 kb on + strand at 19.876 kb on + strand at 19.876 kb on + strand at 19.926 kb on + strand, within GFF1514 at 19.926 kb on + strand, within GFF1514 at 19.927 kb on - strand, within GFF1514 at 19.994 kb on + strand, within GFF1514 at 20.641 kb on + strand, within GFF1515 at 20.801 kb on - strand at 20.862 kb on - strand at 20.865 kb on + strand at 20.865 kb on + strand at 20.865 kb on + strand at 20.878 kb on - strand at 20.880 kb on + strand at 21.071 kb on + strand, within GFF1516 at 21.129 kb on - strand, within GFF1516
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.875 MOI remove 18,880 + GFF1511 0.72 -0.1 18,880 + GFF1511 0.72 -0.4 18,881 - GFF1511 0.72 -1.0 19,024 + -2.0 19,024 + -1.7 19,058 + -1.1 19,225 + GFF1512 0.41 -1.0 19,295 - GFF1512 0.55 -0.6 19,295 - GFF1512 0.55 +0.3 19,295 - GFF1512 0.55 +1.5 19,299 + GFF1512 0.55 -0.5 19,299 + GFF1512 0.55 +2.0 19,366 + GFF1512 0.69 -0.5 19,368 + GFF1512 0.69 -1.1 19,369 - GFF1512 0.69 -1.3 19,369 - GFF1512 0.69 -0.4 19,370 + GFF1512 0.69 -0.6 19,522 - +0.2 19,620 - GFF1513 0.30 +0.6 19,624 + GFF1513 0.31 -0.8 19,782 + GFF1513 0.75 +0.1 19,799 + GFF1513 0.80 -0.2 19,800 - GFF1513 0.80 -0.3 19,861 - +0.6 19,861 - +0.2 19,876 + +0.3 19,876 + +1.2 19,876 + +0.7 19,926 + GFF1514 0.14 +0.4 19,926 + GFF1514 0.14 +1.5 19,927 - GFF1514 0.14 +0.5 19,994 + GFF1514 0.29 +0.6 20,641 + GFF1515 0.68 -1.0 20,801 - -0.7 20,862 - -0.1 20,865 + +0.1 20,865 + +0.2 20,865 + -0.2 20,878 - -0.3 20,880 + +0.5 21,071 + GFF1516 0.21 +0.8 21,129 - GFF1516 0.30 -0.2
Or see this region's nucleotide sequence