Experiment: LB_plus_SM_buffer with EIP16_phage 0.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1471 and GFF1472 are separated by 208 nucleotides GFF1472 and GFF1473 are separated by 327 nucleotides
GFF1471 - Arginine/agmatine antiporter, at 136,292 to 137,560
GFF1471
GFF1472 - Transcriptional regulator of catabolic arginine decarboxylase (adiA), at 137,769 to 138,500
GFF1472
GFF1473 - Arginine decarboxylase, catabolic (EC 4.1.1.19), at 138,828 to 141,098
GFF1473
Position (kb)
137
138
139 Strain fitness (log2 ratio)
-1
0
1
2 at 136.828 kb on + strand, within GFF1471 at 136.915 kb on - strand, within GFF1471 at 137.096 kb on - strand, within GFF1471 at 137.249 kb on - strand, within GFF1471 at 137.258 kb on + strand, within GFF1471 at 137.258 kb on + strand, within GFF1471 at 137.299 kb on - strand, within GFF1471 at 137.409 kb on + strand, within GFF1471 at 137.409 kb on + strand, within GFF1471 at 137.410 kb on - strand, within GFF1471 at 137.410 kb on - strand, within GFF1471 at 137.410 kb on - strand, within GFF1471 at 137.432 kb on + strand, within GFF1471 at 137.669 kb on + strand at 137.676 kb on + strand at 137.676 kb on + strand at 137.706 kb on - strand at 137.736 kb on + strand at 137.736 kb on + strand at 137.804 kb on - strand at 137.822 kb on - strand at 137.866 kb on + strand, within GFF1472 at 138.342 kb on - strand, within GFF1472 at 138.428 kb on + strand at 138.459 kb on - strand at 138.465 kb on + strand at 138.501 kb on + strand at 138.555 kb on + strand at 138.784 kb on - strand at 138.795 kb on - strand at 138.845 kb on + strand at 138.845 kb on + strand at 138.846 kb on - strand at 138.933 kb on + strand at 138.934 kb on - strand at 138.938 kb on - strand at 138.938 kb on - strand at 139.014 kb on - strand at 139.090 kb on + strand, within GFF1473 at 139.205 kb on + strand, within GFF1473 at 139.282 kb on + strand, within GFF1473 at 139.282 kb on + strand, within GFF1473 at 139.361 kb on + strand, within GFF1473 at 139.362 kb on - strand, within GFF1473
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.875 MOI remove 136,828 + GFF1471 0.42 +0.8 136,915 - GFF1471 0.49 -0.1 137,096 - GFF1471 0.63 +0.3 137,249 - GFF1471 0.75 -0.7 137,258 + GFF1471 0.76 -0.8 137,258 + GFF1471 0.76 +0.1 137,299 - GFF1471 0.79 -0.5 137,409 + GFF1471 0.88 -1.5 137,409 + GFF1471 0.88 -0.1 137,410 - GFF1471 0.88 -0.0 137,410 - GFF1471 0.88 -0.5 137,410 - GFF1471 0.88 -0.1 137,432 + GFF1471 0.90 -0.1 137,669 + -0.2 137,676 + -0.3 137,676 + -1.4 137,706 - +0.4 137,736 + -0.4 137,736 + +0.2 137,804 - -0.4 137,822 - +0.5 137,866 + GFF1472 0.13 -0.6 138,342 - GFF1472 0.78 -1.1 138,428 + -0.2 138,459 - -0.4 138,465 + +0.1 138,501 + -0.2 138,555 + +1.3 138,784 - -0.4 138,795 - -1.3 138,845 + +0.2 138,845 + -0.1 138,846 - -0.1 138,933 + +0.1 138,934 - -1.2 138,938 - -0.3 138,938 - -0.1 139,014 - +0.3 139,090 + GFF1473 0.12 +0.6 139,205 + GFF1473 0.17 -0.3 139,282 + GFF1473 0.20 -0.1 139,282 + GFF1473 0.20 -0.2 139,361 + GFF1473 0.23 +2.2 139,362 - GFF1473 0.24 -0.6
Or see this region's nucleotide sequence