Experiment: LB_plus_SM_buffer with EIP16_phage 0.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1457 and GFF1458 overlap by 4 nucleotides GFF1458 and GFF1459 are separated by 139 nucleotides GFF1459 and GFF1460 are separated by 76 nucleotides
GFF1457 - Cytochrome c heme lyase subunit CcmF, at 119,963 to 122,185
GFF1457
GFF1458 - Cytochrome c-type heme lyase subunit nrfG, nitrite reductase complex assembly, at 122,182 to 122,802
GFF1458
GFF1459 - hypothetical protein, at 122,942 to 123,070
GFF1459
GFF1460 - Glutamate-aspartate carrier protein, at 123,147 to 124,457
GFF1460
Position (kb) 122
123
124 Strain fitness (log2 ratio) -1
0
1
2 at 122.003 kb on - strand at 122.229 kb on - strand at 122.452 kb on + strand, within GFF1458 at 122.452 kb on + strand, within GFF1458 at 122.452 kb on + strand, within GFF1458 at 122.577 kb on + strand, within GFF1458 at 122.577 kb on + strand, within GFF1458 at 122.577 kb on + strand, within GFF1458 at 122.578 kb on - strand, within GFF1458 at 122.578 kb on - strand, within GFF1458 at 122.578 kb on - strand, within GFF1458 at 122.607 kb on + strand, within GFF1458 at 122.607 kb on + strand, within GFF1458 at 122.607 kb on + strand, within GFF1458 at 122.608 kb on - strand, within GFF1458 at 123.028 kb on - strand, within GFF1459 at 123.035 kb on - strand, within GFF1459 at 123.088 kb on + strand at 123.089 kb on - strand at 123.092 kb on + strand at 123.093 kb on - strand at 123.093 kb on - strand at 123.228 kb on + strand at 123.285 kb on - strand, within GFF1460 at 123.291 kb on - strand, within GFF1460 at 123.302 kb on + strand, within GFF1460 at 123.485 kb on + strand, within GFF1460 at 123.486 kb on - strand, within GFF1460 at 123.486 kb on - strand, within GFF1460 at 123.541 kb on + strand, within GFF1460 at 123.574 kb on + strand, within GFF1460 at 123.762 kb on + strand, within GFF1460 at 123.994 kb on + strand, within GFF1460 at 123.994 kb on + strand, within GFF1460 at 124.043 kb on + strand, within GFF1460 at 124.043 kb on + strand, within GFF1460
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.875 MOI remove 122,003 - +0.3 122,229 - +0.0 122,452 + GFF1458 0.43 +0.1 122,452 + GFF1458 0.43 +0.1 122,452 + GFF1458 0.43 -0.6 122,577 + GFF1458 0.64 -0.9 122,577 + GFF1458 0.64 +0.2 122,577 + GFF1458 0.64 +0.2 122,578 - GFF1458 0.64 +0.1 122,578 - GFF1458 0.64 +0.3 122,578 - GFF1458 0.64 -0.9 122,607 + GFF1458 0.68 -0.2 122,607 + GFF1458 0.68 -0.3 122,607 + GFF1458 0.68 -0.2 122,608 - GFF1458 0.69 +0.4 123,028 - GFF1459 0.67 -0.9 123,035 - GFF1459 0.72 -0.4 123,088 + +0.6 123,089 - -0.2 123,092 + +2.6 123,093 - -0.2 123,093 - -0.8 123,228 + +0.8 123,285 - GFF1460 0.11 -1.1 123,291 - GFF1460 0.11 +0.1 123,302 + GFF1460 0.12 -0.7 123,485 + GFF1460 0.26 +0.1 123,486 - GFF1460 0.26 -0.4 123,486 - GFF1460 0.26 +0.5 123,541 + GFF1460 0.30 -0.0 123,574 + GFF1460 0.33 -0.8 123,762 + GFF1460 0.47 +1.4 123,994 + GFF1460 0.65 -0.0 123,994 + GFF1460 0.65 -0.2 124,043 + GFF1460 0.68 -0.6 124,043 + GFF1460 0.68 -0.4
Or see this region's nucleotide sequence