Experiment: LB_plus_SM_buffer with EIP16_phage 0.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1400 and GFF1401 are separated by 142 nucleotides GFF1401 and GFF1402 are separated by 165 nucleotides
GFF1400 - YjbH outer membrane lipoprotein, at 44,063 to 46,159
GFF1400
GFF1401 - PsiE protein, at 46,302 to 46,712
GFF1401
GFF1402 - Maltose/maltodextrin ABC transporter, permease protein MalG, at 46,878 to 47,768
GFF1402
Position (kb) 46
47 Strain fitness (log2 ratio) -1
0
1 at 45.312 kb on - strand, within GFF1400 at 45.312 kb on - strand, within GFF1400 at 45.312 kb on - strand, within GFF1400 at 45.314 kb on - strand, within GFF1400 at 45.387 kb on - strand, within GFF1400 at 45.388 kb on + strand, within GFF1400 at 45.388 kb on + strand, within GFF1400 at 45.388 kb on + strand, within GFF1400 at 45.388 kb on + strand, within GFF1400 at 45.388 kb on + strand, within GFF1400 at 45.389 kb on - strand, within GFF1400 at 45.389 kb on - strand, within GFF1400 at 45.392 kb on + strand, within GFF1400 at 45.392 kb on + strand, within GFF1400 at 45.392 kb on + strand, within GFF1400 at 45.393 kb on - strand, within GFF1400 at 45.493 kb on + strand, within GFF1400 at 45.498 kb on - strand, within GFF1400 at 45.538 kb on + strand, within GFF1400 at 45.555 kb on - strand, within GFF1400 at 45.585 kb on + strand, within GFF1400 at 45.586 kb on - strand, within GFF1400 at 45.635 kb on + strand, within GFF1400 at 45.767 kb on - strand, within GFF1400 at 45.786 kb on - strand, within GFF1400 at 45.815 kb on + strand, within GFF1400 at 45.816 kb on - strand, within GFF1400 at 45.952 kb on + strand at 46.002 kb on + strand at 46.127 kb on - strand at 46.127 kb on - strand at 46.208 kb on - strand at 46.240 kb on + strand at 46.264 kb on + strand at 46.465 kb on - strand, within GFF1401 at 46.492 kb on - strand, within GFF1401 at 46.535 kb on + strand, within GFF1401 at 46.633 kb on + strand, within GFF1401 at 46.633 kb on + strand, within GFF1401 at 46.633 kb on + strand, within GFF1401 at 46.633 kb on + strand, within GFF1401 at 46.634 kb on - strand, within GFF1401 at 46.634 kb on - strand, within GFF1401 at 46.636 kb on + strand, within GFF1401 at 46.636 kb on + strand, within GFF1401 at 46.637 kb on - strand, within GFF1401 at 46.668 kb on - strand, within GFF1401 at 46.685 kb on + strand at 46.685 kb on + strand at 46.710 kb on + strand at 46.737 kb on - strand at 46.767 kb on + strand at 46.811 kb on + strand at 46.811 kb on + strand at 46.827 kb on - strand at 46.872 kb on + strand at 47.040 kb on - strand, within GFF1402 at 47.040 kb on - strand, within GFF1402 at 47.041 kb on + strand, within GFF1402 at 47.041 kb on + strand, within GFF1402 at 47.111 kb on + strand, within GFF1402 at 47.111 kb on + strand, within GFF1402 at 47.230 kb on + strand, within GFF1402 at 47.321 kb on - strand, within GFF1402 at 47.321 kb on - strand, within GFF1402 at 47.321 kb on - strand, within GFF1402 at 47.321 kb on - strand, within GFF1402 at 47.321 kb on - strand, within GFF1402 at 47.489 kb on + strand, within GFF1402 at 47.653 kb on - strand, within GFF1402
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.875 MOI remove 45,312 - GFF1400 0.60 -0.0 45,312 - GFF1400 0.60 -0.3 45,312 - GFF1400 0.60 -0.2 45,314 - GFF1400 0.60 +0.2 45,387 - GFF1400 0.63 -0.6 45,388 + GFF1400 0.63 +1.2 45,388 + GFF1400 0.63 +1.7 45,388 + GFF1400 0.63 +0.8 45,388 + GFF1400 0.63 +0.2 45,388 + GFF1400 0.63 +0.1 45,389 - GFF1400 0.63 -0.0 45,389 - GFF1400 0.63 +1.1 45,392 + GFF1400 0.63 -0.1 45,392 + GFF1400 0.63 +0.2 45,392 + GFF1400 0.63 +0.0 45,393 - GFF1400 0.63 +0.1 45,493 + GFF1400 0.68 -0.7 45,498 - GFF1400 0.68 +0.7 45,538 + GFF1400 0.70 -0.2 45,555 - GFF1400 0.71 +0.7 45,585 + GFF1400 0.73 +1.1 45,586 - GFF1400 0.73 +0.2 45,635 + GFF1400 0.75 +0.2 45,767 - GFF1400 0.81 +0.1 45,786 - GFF1400 0.82 +0.3 45,815 + GFF1400 0.84 +0.6 45,816 - GFF1400 0.84 +0.6 45,952 + +1.0 46,002 + +0.1 46,127 - +0.4 46,127 - +0.1 46,208 - -0.1 46,240 + +0.6 46,264 + -0.8 46,465 - GFF1401 0.40 +0.5 46,492 - GFF1401 0.46 +0.2 46,535 + GFF1401 0.57 +1.0 46,633 + GFF1401 0.81 +0.1 46,633 + GFF1401 0.81 +0.2 46,633 + GFF1401 0.81 -0.0 46,633 + GFF1401 0.81 +0.4 46,634 - GFF1401 0.81 -0.3 46,634 - GFF1401 0.81 -0.6 46,636 + GFF1401 0.81 +0.4 46,636 + GFF1401 0.81 +0.2 46,637 - GFF1401 0.82 +0.2 46,668 - GFF1401 0.89 +0.7 46,685 + -0.3 46,685 + -0.4 46,710 + -0.5 46,737 - -0.8 46,767 + +0.7 46,811 + +0.2 46,811 + +0.2 46,827 - -0.6 46,872 + +0.7 47,040 - GFF1402 0.18 +0.3 47,040 - GFF1402 0.18 -0.3 47,041 + GFF1402 0.18 +0.1 47,041 + GFF1402 0.18 -0.9 47,111 + GFF1402 0.26 +0.2 47,111 + GFF1402 0.26 +0.4 47,230 + GFF1402 0.40 -0.1 47,321 - GFF1402 0.50 +0.1 47,321 - GFF1402 0.50 +0.8 47,321 - GFF1402 0.50 +0.2 47,321 - GFF1402 0.50 -0.2 47,321 - GFF1402 0.50 +0.0 47,489 + GFF1402 0.69 +0.6 47,653 - GFF1402 0.87 -0.1
Or see this region's nucleotide sequence