Experiment: LB_plus_SM_buffer with EIP16_phage 0.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF127 and GFF128 are separated by 91 nucleotides GFF128 and GFF129 overlap by 4 nucleotides GFF129 and GFF130 are separated by 87 nucleotides
GFF127 - Alpha-L-arabinofuranosidase II precursor (EC 3.2.1.55), at 117,280 to 118,230
GFF127
GFF128 - AmpE protein, at 118,322 to 119,176
GFF128
GFF129 - N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28) AmpD, at 119,173 to 119,736
GFF129
GFF130 - Quinolinate phosphoribosyltransferase [decarboxylating] (EC 2.4.2.19), at 119,824 to 120,717
GFF130
Position (kb)
119
120 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 118.228 kb on + strand at 118.328 kb on + strand at 118.328 kb on + strand at 118.339 kb on - strand at 118.418 kb on + strand, within GFF128 at 118.479 kb on + strand, within GFF128 at 118.479 kb on + strand, within GFF128 at 118.489 kb on + strand, within GFF128 at 118.490 kb on - strand, within GFF128 at 118.490 kb on - strand, within GFF128 at 118.554 kb on + strand, within GFF128 at 118.554 kb on + strand, within GFF128 at 118.560 kb on - strand, within GFF128 at 118.808 kb on - strand, within GFF128 at 118.808 kb on - strand, within GFF128 at 118.905 kb on + strand, within GFF128 at 118.906 kb on - strand, within GFF128 at 118.911 kb on - strand, within GFF128 at 118.934 kb on - strand, within GFF128 at 118.983 kb on - strand, within GFF128 at 118.994 kb on + strand, within GFF128 at 119.084 kb on + strand, within GFF128 at 119.156 kb on + strand at 119.164 kb on + strand at 119.307 kb on - strand, within GFF129 at 119.349 kb on - strand, within GFF129 at 119.477 kb on + strand, within GFF129 at 119.477 kb on + strand, within GFF129 at 119.477 kb on + strand, within GFF129 at 119.478 kb on - strand, within GFF129 at 119.632 kb on - strand, within GFF129 at 119.634 kb on + strand, within GFF129 at 119.634 kb on + strand, within GFF129 at 119.634 kb on + strand, within GFF129 at 119.634 kb on + strand, within GFF129 at 119.635 kb on - strand, within GFF129 at 119.764 kb on + strand at 119.765 kb on - strand at 119.765 kb on - strand at 119.778 kb on + strand at 119.794 kb on + strand at 119.795 kb on - strand at 119.839 kb on + strand at 119.839 kb on + strand at 119.982 kb on + strand, within GFF130 at 120.316 kb on + strand, within GFF130 at 120.391 kb on - strand, within GFF130 at 120.440 kb on + strand, within GFF130 at 120.516 kb on + strand, within GFF130
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.875 MOI remove 118,228 + +0.7 118,328 + +0.9 118,328 + -0.3 118,339 - +0.3 118,418 + GFF128 0.11 -0.3 118,479 + GFF128 0.18 -0.3 118,479 + GFF128 0.18 +0.6 118,489 + GFF128 0.20 +0.3 118,490 - GFF128 0.20 -0.6 118,490 - GFF128 0.20 +0.3 118,554 + GFF128 0.27 +0.4 118,554 + GFF128 0.27 +0.3 118,560 - GFF128 0.28 -0.1 118,808 - GFF128 0.57 +0.1 118,808 - GFF128 0.57 -0.3 118,905 + GFF128 0.68 -0.2 118,906 - GFF128 0.68 +0.3 118,911 - GFF128 0.69 +0.1 118,934 - GFF128 0.72 +0.2 118,983 - GFF128 0.77 +0.4 118,994 + GFF128 0.79 +0.2 119,084 + GFF128 0.89 -2.0 119,156 + +0.1 119,164 + -0.3 119,307 - GFF129 0.24 +0.4 119,349 - GFF129 0.31 +0.8 119,477 + GFF129 0.54 +0.7 119,477 + GFF129 0.54 +0.2 119,477 + GFF129 0.54 +0.1 119,478 - GFF129 0.54 +0.4 119,632 - GFF129 0.81 +1.5 119,634 + GFF129 0.82 -0.3 119,634 + GFF129 0.82 +0.2 119,634 + GFF129 0.82 +0.5 119,634 + GFF129 0.82 -0.0 119,635 - GFF129 0.82 +0.1 119,764 + +0.3 119,765 - -0.7 119,765 - +0.1 119,778 + +0.5 119,794 + +0.4 119,795 - +0.5 119,839 + +0.2 119,839 + +2.2 119,982 + GFF130 0.18 +1.0 120,316 + GFF130 0.55 +0.1 120,391 - GFF130 0.63 -0.3 120,440 + GFF130 0.69 -0.8 120,516 + GFF130 0.77 +0.2
Or see this region's nucleotide sequence