Experiment: LB_plus_SM_buffer with EIP16_phage 0.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1238 and GFF1239 are separated by 173 nucleotides GFF1239 and GFF1240 are separated by 173 nucleotides GFF1240 and GFF1241 are separated by 269 nucleotides GFF1241 and GFF1242 are separated by 207 nucleotides
GFF1238 - 28.1 kDa virulence protein, at 70,365 to 71,132
GFF1238
GFF1239 - Gifsy-2 prophage lysozyme( EC:3.2.1.17 ), at 71,306 to 71,470
GFF1239
GFF1240 - Virulence genes transcriptional activator, at 71,644 to 72,537
GFF1240
GFF1241 - hypothetical protein, at 72,807 to 72,938
GFF1241
GFF1242 - Mobile element protein, at 73,146 to 74,183
GFF1242
Position (kb) 71
72
73 Strain fitness (log2 ratio) -1
0
1
2 at 70.664 kb on + strand, within GFF1238 at 70.665 kb on - strand, within GFF1238 at 70.665 kb on - strand, within GFF1238 at 70.701 kb on + strand, within GFF1238 at 70.729 kb on + strand, within GFF1238 at 70.927 kb on + strand, within GFF1238 at 70.928 kb on - strand, within GFF1238 at 70.989 kb on - strand, within GFF1238 at 70.989 kb on - strand, within GFF1238 at 71.132 kb on + strand at 71.318 kb on + strand at 71.412 kb on + strand, within GFF1239 at 71.461 kb on + strand at 71.476 kb on - strand at 71.479 kb on + strand at 71.536 kb on - strand at 71.557 kb on + strand at 71.558 kb on - strand at 71.558 kb on - strand at 71.683 kb on + strand at 71.686 kb on - strand at 71.729 kb on + strand at 71.740 kb on - strand, within GFF1240 at 71.819 kb on - strand, within GFF1240 at 71.872 kb on - strand, within GFF1240 at 71.895 kb on + strand, within GFF1240 at 71.899 kb on - strand, within GFF1240 at 71.902 kb on + strand, within GFF1240 at 72.451 kb on - strand at 72.458 kb on - strand at 72.687 kb on - strand at 72.732 kb on + strand at 72.732 kb on + strand at 72.732 kb on + strand at 72.733 kb on - strand at 72.756 kb on - strand at 72.883 kb on + strand, within GFF1241 at 72.902 kb on + strand, within GFF1241 at 72.937 kb on - strand at 73.049 kb on - strand at 73.049 kb on - strand at 73.049 kb on - strand at 73.062 kb on - strand at 73.062 kb on - strand at 73.156 kb on + strand at 73.156 kb on + strand at 73.156 kb on + strand at 73.328 kb on - strand, within GFF1242 at 73.342 kb on + strand, within GFF1242 at 73.361 kb on - strand, within GFF1242 at 73.407 kb on - strand, within GFF1242 at 73.432 kb on + strand, within GFF1242
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.875 MOI remove 70,664 + GFF1238 0.39 -0.6 70,665 - GFF1238 0.39 -0.5 70,665 - GFF1238 0.39 +0.1 70,701 + GFF1238 0.44 +0.5 70,729 + GFF1238 0.47 -0.2 70,927 + GFF1238 0.73 +0.8 70,928 - GFF1238 0.73 -0.7 70,989 - GFF1238 0.81 +0.5 70,989 - GFF1238 0.81 -0.8 71,132 + -0.6 71,318 + +0.1 71,412 + GFF1239 0.64 +0.3 71,461 + +0.3 71,476 - +0.4 71,479 + -0.5 71,536 - +0.6 71,557 + +0.4 71,558 - +0.2 71,558 - -0.4 71,683 + -0.3 71,686 - -0.9 71,729 + +0.2 71,740 - GFF1240 0.11 +0.0 71,819 - GFF1240 0.20 +0.3 71,872 - GFF1240 0.26 -0.0 71,895 + GFF1240 0.28 -0.0 71,899 - GFF1240 0.29 -1.0 71,902 + GFF1240 0.29 -0.4 72,451 - +0.3 72,458 - -0.6 72,687 - -0.4 72,732 + +2.0 72,732 + +0.6 72,732 + +0.6 72,733 - +0.1 72,756 - +0.6 72,883 + GFF1241 0.58 -0.2 72,902 + GFF1241 0.72 -0.6 72,937 - -0.2 73,049 - +0.9 73,049 - +2.1 73,049 - +0.5 73,062 - +1.0 73,062 - +0.2 73,156 + -0.1 73,156 + +0.2 73,156 + -0.4 73,328 - GFF1242 0.18 -0.3 73,342 + GFF1242 0.19 +0.2 73,361 - GFF1242 0.21 +0.1 73,407 - GFF1242 0.25 -1.3 73,432 + GFF1242 0.28 +0.0
Or see this region's nucleotide sequence