Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF1186

Experiment: LB_plus_SM_buffer with EIP16_phage 0.875 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1185 and GFF1186 are separated by 8 nucleotidesGFF1186 and GFF1187 overlap by 1 nucleotidesGFF1187 and GFF1188 are separated by 78 nucleotides GFF1185 - IncF plasmid conjugative transfer protein TraD, at 30,607 to 32,850 GFF1185 GFF1186 - VapC toxin protein, at 32,859 to 33,257 GFF1186 GFF1187 - VapB protein (antitoxin to VapC), at 33,257 to 33,487 GFF1187 GFF1188 - IncF plasmid conjugative transfer DNA-nicking and unwinding protein TraI, at 33,566 to 38,824 GFF1188 Position (kb) 32 33 34Strain fitness (log2 ratio) -2 -1 0 1 2 3at 31.955 kb on - strand, within GFF1185at 31.993 kb on - strand, within GFF1185at 31.993 kb on - strand, within GFF1185at 31.996 kb on + strand, within GFF1185at 31.996 kb on + strand, within GFF1185at 31.996 kb on + strand, within GFF1185at 31.997 kb on - strand, within GFF1185at 31.997 kb on - strand, within GFF1185at 32.066 kb on - strand, within GFF1185at 32.067 kb on + strand, within GFF1185at 32.067 kb on + strand, within GFF1185at 32.067 kb on + strand, within GFF1185at 32.067 kb on + strand, within GFF1185at 32.081 kb on - strand, within GFF1185at 32.081 kb on - strand, within GFF1185at 32.137 kb on + strand, within GFF1185at 32.207 kb on - strand, within GFF1185at 32.243 kb on - strand, within GFF1185at 32.290 kb on + strand, within GFF1185at 32.617 kb on - strand, within GFF1185at 32.691 kb on + strandat 32.725 kb on + strandat 32.793 kb on + strandat 33.044 kb on - strand, within GFF1186at 33.124 kb on + strand, within GFF1186at 33.156 kb on - strand, within GFF1186at 33.233 kb on + strandat 33.234 kb on - strandat 33.234 kb on - strandat 33.236 kb on + strandat 33.246 kb on - strandat 33.304 kb on + strand, within GFF1187at 33.368 kb on - strand, within GFF1187at 33.470 kb on + strandat 33.471 kb on - strandat 33.505 kb on + strandat 33.506 kb on - strandat 33.512 kb on + strandat 33.513 kb on - strandat 33.517 kb on - strandat 33.574 kb on + strandat 33.575 kb on - strandat 33.614 kb on + strandat 33.615 kb on - strandat 33.628 kb on + strandat 33.628 kb on + strandat 33.628 kb on + strandat 33.628 kb on + strandat 33.628 kb on + strandat 33.629 kb on - strandat 33.632 kb on + strandat 33.633 kb on - strandat 33.635 kb on + strandat 33.636 kb on - strandat 33.636 kb on - strandat 33.636 kb on - strandat 33.636 kb on - strandat 33.862 kb on + strandat 33.863 kb on - strandat 34.037 kb on - strandat 34.121 kb on - strand, within GFF1188at 34.133 kb on + strand, within GFF1188at 34.134 kb on - strand, within GFF1188at 34.134 kb on - strand, within GFF1188at 34.134 kb on - strand, within GFF1188at 34.147 kb on - strand, within GFF1188at 34.231 kb on + strand, within GFF1188at 34.231 kb on + strand, within GFF1188at 34.231 kb on + strand, within GFF1188

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.875 MOI
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31,955 - GFF1185 0.60 -0.6
31,993 - GFF1185 0.62 +0.2
31,993 - GFF1185 0.62 +0.8
31,996 + GFF1185 0.62 -0.9
31,996 + GFF1185 0.62 +0.1
31,996 + GFF1185 0.62 +0.0
31,997 - GFF1185 0.62 +0.2
31,997 - GFF1185 0.62 +0.2
32,066 - GFF1185 0.65 +0.2
32,067 + GFF1185 0.65 +0.5
32,067 + GFF1185 0.65 -1.9
32,067 + GFF1185 0.65 +0.3
32,067 + GFF1185 0.65 +1.1
32,081 - GFF1185 0.66 -0.2
32,081 - GFF1185 0.66 +0.8
32,137 + GFF1185 0.68 +0.5
32,207 - GFF1185 0.71 -0.1
32,243 - GFF1185 0.73 +2.8
32,290 + GFF1185 0.75 -1.2
32,617 - GFF1185 0.90 -0.1
32,691 + -0.0
32,725 + +0.4
32,793 + +0.1
33,044 - GFF1186 0.46 -0.5
33,124 + GFF1186 0.66 -0.4
33,156 - GFF1186 0.74 -1.6
33,233 + -0.4
33,234 - -0.2
33,234 - -0.2
33,236 + +0.1
33,246 - +0.4
33,304 + GFF1187 0.20 +0.7
33,368 - GFF1187 0.48 +0.3
33,470 + -0.5
33,471 - +2.0
33,505 + +1.0
33,506 - -0.2
33,512 + -0.0
33,513 - +0.7
33,517 - -1.0
33,574 + -0.2
33,575 - -2.1
33,614 + +2.3
33,615 - +0.6
33,628 + -0.7
33,628 + +0.6
33,628 + +1.0
33,628 + +0.7
33,628 + -0.7
33,629 - -0.1
33,632 + -0.2
33,633 - +0.6
33,635 + +0.1
33,636 - -0.8
33,636 - -0.5
33,636 - -0.2
33,636 - -1.2
33,862 + +0.3
33,863 - -0.5
34,037 - +0.4
34,121 - GFF1188 0.11 +0.2
34,133 + GFF1188 0.11 +0.2
34,134 - GFF1188 0.11 -0.4
34,134 - GFF1188 0.11 -0.7
34,134 - GFF1188 0.11 +0.7
34,147 - GFF1188 0.11 -0.1
34,231 + GFF1188 0.13 +0.3
34,231 + GFF1188 0.13 -1.9
34,231 + GFF1188 0.13 +0.5

Or see this region's nucleotide sequence