Experiment: LB_plus_SM_buffer with EIP16_phage 0.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1061 and GFF1062 are separated by 101 nucleotides GFF1062 and GFF1063 are separated by 48 nucleotides GFF1063 and GFF1064 overlap by 4 nucleotides
GFF1061 - Transcriptional repressor protein TrpR, at 244,382 to 244,708
GFF1061
GFF1062 - Inosine/xanthosine triphosphatase (EC 3.6.1.-), at 244,810 to 245,325
GFF1062
GFF1063 - Phosphoglycerate mutase (EC 5.4.2.1), at 245,374 to 246,021
GFF1063
GFF1064 - Right origin-binding protein, at 246,018 to 246,887
GFF1064
Position (kb) 245
246
247 Strain fitness (log2 ratio) -1
0
1
2 at 244.376 kb on + strand at 244.426 kb on + strand, within GFF1061 at 244.455 kb on + strand, within GFF1061 at 244.495 kb on - strand, within GFF1061 at 244.552 kb on + strand, within GFF1061 at 244.553 kb on - strand, within GFF1061 at 244.718 kb on - strand at 244.796 kb on + strand at 244.797 kb on - strand at 244.865 kb on + strand, within GFF1062 at 244.938 kb on + strand, within GFF1062 at 244.938 kb on + strand, within GFF1062 at 244.938 kb on + strand, within GFF1062 at 245.146 kb on - strand, within GFF1062 at 245.230 kb on - strand, within GFF1062 at 245.233 kb on - strand, within GFF1062 at 245.386 kb on + strand at 245.523 kb on + strand, within GFF1063 at 245.540 kb on + strand, within GFF1063 at 245.856 kb on - strand, within GFF1063 at 245.856 kb on - strand, within GFF1063 at 246.012 kb on - strand at 246.097 kb on + strand at 246.106 kb on - strand, within GFF1064 at 246.106 kb on - strand, within GFF1064 at 246.107 kb on + strand, within GFF1064 at 246.146 kb on + strand, within GFF1064 at 246.202 kb on + strand, within GFF1064 at 246.217 kb on + strand, within GFF1064 at 246.296 kb on - strand, within GFF1064 at 246.301 kb on - strand, within GFF1064 at 246.352 kb on + strand, within GFF1064 at 246.368 kb on + strand, within GFF1064 at 246.369 kb on - strand, within GFF1064 at 246.455 kb on - strand, within GFF1064 at 246.455 kb on - strand, within GFF1064 at 246.483 kb on - strand, within GFF1064 at 246.731 kb on - strand, within GFF1064
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.875 MOI remove 244,376 + -0.9 244,426 + GFF1061 0.13 -0.2 244,455 + GFF1061 0.22 +0.2 244,495 - GFF1061 0.35 +0.7 244,552 + GFF1061 0.52 +0.2 244,553 - GFF1061 0.52 -0.1 244,718 - -0.0 244,796 + -0.5 244,797 - +0.4 244,865 + GFF1062 0.11 -0.5 244,938 + GFF1062 0.25 -0.6 244,938 + GFF1062 0.25 -0.3 244,938 + GFF1062 0.25 +2.1 245,146 - GFF1062 0.65 -0.2 245,230 - GFF1062 0.81 +0.7 245,233 - GFF1062 0.82 +1.9 245,386 + +0.5 245,523 + GFF1063 0.23 +0.9 245,540 + GFF1063 0.26 +0.2 245,856 - GFF1063 0.74 +0.6 245,856 - GFF1063 0.74 -0.7 246,012 - +0.3 246,097 + +0.6 246,106 - GFF1064 0.10 +0.1 246,106 - GFF1064 0.10 -0.3 246,107 + GFF1064 0.10 +0.2 246,146 + GFF1064 0.15 -0.2 246,202 + GFF1064 0.21 +0.6 246,217 + GFF1064 0.23 -0.1 246,296 - GFF1064 0.32 +0.1 246,301 - GFF1064 0.33 +0.9 246,352 + GFF1064 0.38 +2.1 246,368 + GFF1064 0.40 +0.2 246,369 - GFF1064 0.40 -0.2 246,455 - GFF1064 0.50 +0.0 246,455 - GFF1064 0.50 +0.7 246,483 - GFF1064 0.53 -0.1 246,731 - GFF1064 0.82 +0.4
Or see this region's nucleotide sequence