Experiment: LB_plus_SM_buffer with EIP16_phage 0.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1057 and GFF1058 are separated by 150 nucleotides GFF1058 and GFF1059 are separated by 117 nucleotides
GFF1057 - DNA repair protein RadA, at 237,628 to 239,010
GFF1057
GFF1058 - NadR transcriptional regulator / Nicotinamide-nucleotide adenylyltransferase, NadR family (EC 2.7.7.1) / Ribosylnicotinamide kinase (EC 2.7.1.22), at 239,161 to 240,393
GFF1058
GFF1059 - ABC transporter, ATP-binding protein, at 240,511 to 242,178
GFF1059
Position (kb) 239
240
241 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 238.164 kb on - strand, within GFF1057 at 238.176 kb on + strand, within GFF1057 at 238.873 kb on - strand at 238.988 kb on + strand at 239.007 kb on - strand at 239.019 kb on - strand at 239.022 kb on + strand at 239.022 kb on + strand at 239.023 kb on - strand at 239.034 kb on - strand at 239.133 kb on + strand at 239.177 kb on - strand at 239.392 kb on - strand, within GFF1058 at 239.608 kb on + strand, within GFF1058 at 239.686 kb on + strand, within GFF1058 at 239.717 kb on - strand, within GFF1058 at 239.824 kb on - strand, within GFF1058 at 239.856 kb on + strand, within GFF1058 at 239.948 kb on - strand, within GFF1058 at 239.959 kb on + strand, within GFF1058 at 239.960 kb on - strand, within GFF1058 at 240.170 kb on - strand, within GFF1058 at 240.201 kb on + strand, within GFF1058 at 240.201 kb on + strand, within GFF1058 at 240.428 kb on - strand at 240.523 kb on - strand at 240.591 kb on - strand at 240.690 kb on + strand, within GFF1059 at 240.691 kb on - strand, within GFF1059 at 240.895 kb on + strand, within GFF1059 at 240.895 kb on + strand, within GFF1059 at 240.895 kb on + strand, within GFF1059 at 240.895 kb on + strand, within GFF1059 at 240.895 kb on + strand, within GFF1059 at 241.242 kb on + strand, within GFF1059
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.875 MOI remove 238,164 - GFF1057 0.39 +0.7 238,176 + GFF1057 0.40 -0.2 238,873 - +0.7 238,988 + +0.3 239,007 - +1.8 239,019 - +0.3 239,022 + -0.9 239,022 + -0.4 239,023 - -0.3 239,034 - -0.2 239,133 + -1.6 239,177 - +2.2 239,392 - GFF1058 0.19 -1.2 239,608 + GFF1058 0.36 -1.2 239,686 + GFF1058 0.43 -1.3 239,717 - GFF1058 0.45 -2.3 239,824 - GFF1058 0.54 -0.7 239,856 + GFF1058 0.56 -0.9 239,948 - GFF1058 0.64 -2.8 239,959 + GFF1058 0.65 -0.4 239,960 - GFF1058 0.65 -0.9 240,170 - GFF1058 0.82 -1.2 240,201 + GFF1058 0.84 -1.3 240,201 + GFF1058 0.84 -0.5 240,428 - -2.2 240,523 - +0.9 240,591 - +0.0 240,690 + GFF1059 0.11 +1.8 240,691 - GFF1059 0.11 +0.8 240,895 + GFF1059 0.23 +0.5 240,895 + GFF1059 0.23 +1.1 240,895 + GFF1059 0.23 +0.6 240,895 + GFF1059 0.23 +2.6 240,895 + GFF1059 0.23 +1.1 241,242 + GFF1059 0.44 +1.7
Or see this region's nucleotide sequence