Experiment: LB_plus_SM_buffer with EIP16_phage 0.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1045 and GFF1046 are separated by 209 nucleotides GFF1046 and GFF1047 are separated by 37 nucleotides GFF1047 and GFF1048 overlap by 4 nucleotides
GFF1045 - Phosphopentomutase (EC 5.4.2.7), at 227,060 to 228,283
GFF1045
GFF1046 - Purine nucleoside phosphorylase (EC 2.4.2.1), at 228,493 to 229,212
GFF1046
GFF1047 - Beta-fimbriae probable major subunit, at 229,250 to 229,822
GFF1047
GFF1048 - Beta-fimbriae usher protein, at 229,819 to 232,227
GFF1048
Position (kb) 228
229
230 Strain fitness (log2 ratio) -1
0
1
2 at 227.631 kb on - strand, within GFF1045 at 227.675 kb on + strand, within GFF1045 at 227.849 kb on + strand, within GFF1045 at 227.850 kb on - strand, within GFF1045 at 227.850 kb on - strand, within GFF1045 at 228.128 kb on - strand, within GFF1045 at 228.192 kb on + strand at 228.248 kb on - strand at 228.306 kb on - strand at 228.341 kb on + strand at 228.341 kb on + strand at 228.342 kb on - strand at 228.342 kb on - strand at 228.342 kb on - strand at 228.345 kb on + strand at 228.345 kb on + strand at 228.346 kb on - strand at 228.346 kb on - strand at 228.350 kb on + strand at 228.406 kb on + strand at 228.507 kb on + strand at 228.507 kb on + strand at 228.508 kb on - strand at 228.542 kb on + strand at 228.542 kb on + strand at 228.632 kb on + strand, within GFF1046 at 228.650 kb on - strand, within GFF1046 at 228.814 kb on - strand, within GFF1046 at 228.855 kb on + strand, within GFF1046 at 228.974 kb on - strand, within GFF1046 at 229.028 kb on + strand, within GFF1046 at 229.047 kb on + strand, within GFF1046 at 229.117 kb on - strand, within GFF1046 at 229.204 kb on - strand at 229.286 kb on + strand at 229.286 kb on + strand at 229.286 kb on + strand at 229.287 kb on - strand at 229.287 kb on - strand at 229.341 kb on - strand, within GFF1047 at 229.385 kb on - strand, within GFF1047 at 229.388 kb on - strand, within GFF1047 at 229.411 kb on + strand, within GFF1047 at 229.411 kb on + strand, within GFF1047 at 229.419 kb on - strand, within GFF1047 at 229.489 kb on - strand, within GFF1047 at 229.502 kb on + strand, within GFF1047 at 229.503 kb on - strand, within GFF1047 at 229.597 kb on + strand, within GFF1047 at 229.597 kb on + strand, within GFF1047 at 229.767 kb on - strand at 229.781 kb on - strand at 229.787 kb on + strand at 229.787 kb on + strand at 229.789 kb on + strand at 229.789 kb on + strand at 229.928 kb on - strand at 229.992 kb on - strand at 229.996 kb on - strand at 230.009 kb on - strand at 230.009 kb on - strand at 230.053 kb on + strand at 230.053 kb on + strand at 230.179 kb on - strand, within GFF1048
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 0.875 MOI remove 227,631 - GFF1045 0.47 +0.1 227,675 + GFF1045 0.50 +0.8 227,849 + GFF1045 0.64 +0.4 227,850 - GFF1045 0.65 +2.3 227,850 - GFF1045 0.65 +0.3 228,128 - GFF1045 0.87 +1.1 228,192 + +0.5 228,248 - +2.0 228,306 - +0.9 228,341 + -0.7 228,341 + -0.8 228,342 - +0.6 228,342 - +0.7 228,342 - +0.1 228,345 + -0.3 228,345 + -0.0 228,346 - +0.6 228,346 - +1.0 228,350 + -1.4 228,406 + -0.5 228,507 + +0.5 228,507 + +0.9 228,508 - +0.6 228,542 + +1.4 228,542 + +0.5 228,632 + GFF1046 0.19 +1.0 228,650 - GFF1046 0.22 +0.7 228,814 - GFF1046 0.45 +0.4 228,855 + GFF1046 0.50 +0.5 228,974 - GFF1046 0.67 -0.2 229,028 + GFF1046 0.74 +1.1 229,047 + GFF1046 0.77 +0.9 229,117 - GFF1046 0.87 +0.4 229,204 - +0.3 229,286 + -0.2 229,286 + +0.1 229,286 + -0.4 229,287 - -0.5 229,287 - -0.7 229,341 - GFF1047 0.16 -0.8 229,385 - GFF1047 0.24 -0.3 229,388 - GFF1047 0.24 -0.7 229,411 + GFF1047 0.28 +0.3 229,411 + GFF1047 0.28 -1.0 229,419 - GFF1047 0.29 -0.3 229,489 - GFF1047 0.42 +0.9 229,502 + GFF1047 0.44 -0.1 229,503 - GFF1047 0.44 -0.3 229,597 + GFF1047 0.61 +0.3 229,597 + GFF1047 0.61 -0.9 229,767 - +0.3 229,781 - +0.6 229,787 + -0.2 229,787 + +0.0 229,789 + -1.5 229,789 + -0.2 229,928 - +1.2 229,992 - +1.2 229,996 - +1.3 230,009 - -0.1 230,009 - +2.2 230,053 + +0.5 230,053 + +0.4 230,179 - GFF1048 0.15 +0.6
Or see this region's nucleotide sequence