Experiment: R2A with Carbenicillin 150 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu3471 and Atu3472 are separated by 2 nucleotides Atu3472 and Atu3473 are separated by 3 nucleotides
Atu3471: Atu3471 - branched-chain alpha-keto acid dehydrogenase subunit E2, at 517,905 to 519,182
Atu3471
Atu3472: Atu3472 - 2-oxoisovalerate dehydrogenase subunit beta, at 519,185 to 520,198
Atu3472
Atu3473: Atu3473 - 2-oxoisovalerate dehydrogenase subunit alpha, at 520,202 to 521,440
Atu3473
Position (kb)
519
520
521 Strain fitness (log2 ratio)
-2
-1
0
1 at 518.228 kb on + strand, within Atu3471 at 518.248 kb on - strand, within Atu3471 at 518.248 kb on - strand, within Atu3471 at 518.288 kb on - strand, within Atu3471 at 518.311 kb on + strand, within Atu3471 at 518.344 kb on + strand, within Atu3471 at 518.409 kb on + strand, within Atu3471 at 518.607 kb on - strand, within Atu3471 at 518.612 kb on - strand, within Atu3471 at 518.701 kb on + strand, within Atu3471 at 518.701 kb on + strand, within Atu3471 at 518.707 kb on + strand, within Atu3471 at 518.709 kb on - strand, within Atu3471 at 518.715 kb on - strand, within Atu3471 at 518.797 kb on + strand, within Atu3471 at 518.840 kb on - strand, within Atu3471 at 518.927 kb on - strand, within Atu3471 at 518.927 kb on - strand, within Atu3471 at 519.069 kb on + strand at 519.077 kb on - strand at 519.191 kb on - strand at 519.419 kb on - strand, within Atu3472 at 519.485 kb on - strand, within Atu3472 at 519.699 kb on + strand, within Atu3472 at 519.700 kb on + strand, within Atu3472 at 519.774 kb on - strand, within Atu3472 at 519.930 kb on - strand, within Atu3472 at 519.984 kb on - strand, within Atu3472 at 520.078 kb on - strand, within Atu3472 at 520.149 kb on + strand at 520.381 kb on + strand, within Atu3473 at 520.402 kb on + strand, within Atu3473 at 520.403 kb on - strand, within Atu3473 at 520.461 kb on + strand, within Atu3473 at 520.501 kb on - strand, within Atu3473 at 520.534 kb on + strand, within Atu3473 at 520.542 kb on - strand, within Atu3473 at 520.547 kb on - strand, within Atu3473 at 520.572 kb on - strand, within Atu3473 at 520.583 kb on + strand, within Atu3473 at 520.583 kb on + strand, within Atu3473 at 520.591 kb on - strand, within Atu3473 at 520.591 kb on - strand, within Atu3473 at 520.629 kb on - strand, within Atu3473 at 520.739 kb on - strand, within Atu3473 at 520.931 kb on - strand, within Atu3473 at 520.979 kb on - strand, within Atu3473 at 521.134 kb on - strand, within Atu3473
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Carbenicillin 150 mM remove 518,228 + Atu3471 0.25 -0.5 518,248 - Atu3471 0.27 +0.0 518,248 - Atu3471 0.27 -0.3 518,288 - Atu3471 0.30 -0.5 518,311 + Atu3471 0.32 -0.5 518,344 + Atu3471 0.34 -1.6 518,409 + Atu3471 0.39 -0.2 518,607 - Atu3471 0.55 +0.3 518,612 - Atu3471 0.55 -0.8 518,701 + Atu3471 0.62 +0.9 518,701 + Atu3471 0.62 -1.0 518,707 + Atu3471 0.63 -0.1 518,709 - Atu3471 0.63 -0.5 518,715 - Atu3471 0.63 +0.0 518,797 + Atu3471 0.70 -1.2 518,840 - Atu3471 0.73 -1.2 518,927 - Atu3471 0.80 -1.8 518,927 - Atu3471 0.80 -1.4 519,069 + +0.2 519,077 - -0.4 519,191 - -0.6 519,419 - Atu3472 0.23 -0.9 519,485 - Atu3472 0.30 +0.0 519,699 + Atu3472 0.51 -0.3 519,700 + Atu3472 0.51 +0.6 519,774 - Atu3472 0.58 -1.1 519,930 - Atu3472 0.73 -0.1 519,984 - Atu3472 0.79 -0.3 520,078 - Atu3472 0.88 -0.4 520,149 + -0.6 520,381 + Atu3473 0.14 -0.5 520,402 + Atu3473 0.16 -1.0 520,403 - Atu3473 0.16 -1.1 520,461 + Atu3473 0.21 -0.3 520,501 - Atu3473 0.24 -0.8 520,534 + Atu3473 0.27 +0.3 520,542 - Atu3473 0.27 -2.0 520,547 - Atu3473 0.28 -0.4 520,572 - Atu3473 0.30 -0.1 520,583 + Atu3473 0.31 +0.2 520,583 + Atu3473 0.31 -0.6 520,591 - Atu3473 0.31 -0.5 520,591 - Atu3473 0.31 -1.3 520,629 - Atu3473 0.34 -0.8 520,739 - Atu3473 0.43 -0.9 520,931 - Atu3473 0.59 -1.2 520,979 - Atu3473 0.63 +0.3 521,134 - Atu3473 0.75 -0.9
Or see this region's nucleotide sequence