Experiment: R2A with Carbenicillin 150 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu3057 and Atu3059 are separated by 423 nucleotides Atu3059 and Atu3060 are separated by 282 nucleotides
Atu3057: Atu3057 - hypothetical protein, at 56,941 to 58,398
Atu3057
Atu3059: Atu3059 - Na+/H+ antiporter, at 58,822 to 60,405
Atu3059
Atu3060: Atu3060 - ABC transporter permease, at 60,688 to 62,652
Atu3060
Position (kb)
58
59
60
61 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 57.885 kb on - strand, within Atu3057 at 58.063 kb on + strand, within Atu3057 at 58.101 kb on - strand, within Atu3057 at 58.133 kb on - strand, within Atu3057 at 58.139 kb on - strand, within Atu3057 at 58.146 kb on + strand, within Atu3057 at 58.146 kb on + strand, within Atu3057 at 58.146 kb on + strand, within Atu3057 at 58.187 kb on + strand, within Atu3057 at 58.195 kb on - strand, within Atu3057 at 58.195 kb on - strand, within Atu3057 at 58.257 kb on + strand at 58.334 kb on + strand at 58.342 kb on - strand at 58.403 kb on - strand at 58.440 kb on + strand at 58.448 kb on - strand at 58.546 kb on + strand at 58.636 kb on + strand at 58.751 kb on + strand at 58.807 kb on + strand at 58.815 kb on - strand at 58.819 kb on + strand at 58.819 kb on + strand at 58.889 kb on - strand at 58.889 kb on - strand at 58.906 kb on - strand at 58.913 kb on + strand at 58.926 kb on + strand at 58.935 kb on + strand at 59.037 kb on + strand, within Atu3059 at 59.096 kb on + strand, within Atu3059 at 59.106 kb on + strand, within Atu3059 at 59.106 kb on + strand, within Atu3059 at 59.106 kb on + strand, within Atu3059 at 59.114 kb on - strand, within Atu3059 at 59.114 kb on - strand, within Atu3059 at 59.157 kb on + strand, within Atu3059 at 59.165 kb on - strand, within Atu3059 at 59.224 kb on + strand, within Atu3059 at 59.227 kb on - strand, within Atu3059 at 59.244 kb on + strand, within Atu3059 at 59.490 kb on + strand, within Atu3059 at 59.490 kb on + strand, within Atu3059 at 59.490 kb on + strand, within Atu3059 at 59.490 kb on + strand, within Atu3059 at 59.490 kb on + strand, within Atu3059 at 59.490 kb on + strand, within Atu3059 at 59.490 kb on + strand, within Atu3059 at 59.490 kb on + strand, within Atu3059 at 59.490 kb on + strand, within Atu3059 at 59.493 kb on - strand, within Atu3059 at 59.498 kb on - strand, within Atu3059 at 59.507 kb on + strand, within Atu3059 at 59.516 kb on + strand, within Atu3059 at 59.520 kb on + strand, within Atu3059 at 59.528 kb on + strand, within Atu3059 at 59.536 kb on - strand, within Atu3059 at 59.548 kb on - strand, within Atu3059 at 59.581 kb on - strand, within Atu3059 at 59.632 kb on + strand, within Atu3059 at 59.632 kb on + strand, within Atu3059 at 59.635 kb on - strand, within Atu3059 at 59.640 kb on - strand, within Atu3059 at 59.640 kb on - strand, within Atu3059 at 59.650 kb on - strand, within Atu3059 at 59.711 kb on + strand, within Atu3059 at 59.764 kb on - strand, within Atu3059 at 59.844 kb on + strand, within Atu3059 at 59.851 kb on - strand, within Atu3059 at 59.852 kb on - strand, within Atu3059 at 59.852 kb on - strand, within Atu3059 at 59.868 kb on + strand, within Atu3059 at 59.880 kb on + strand, within Atu3059 at 59.880 kb on + strand, within Atu3059 at 59.898 kb on - strand, within Atu3059 at 59.915 kb on + strand, within Atu3059 at 59.972 kb on - strand, within Atu3059 at 59.972 kb on - strand, within Atu3059 at 60.006 kb on + strand, within Atu3059 at 60.022 kb on - strand, within Atu3059 at 60.041 kb on + strand, within Atu3059 at 60.049 kb on - strand, within Atu3059 at 60.050 kb on + strand, within Atu3059 at 60.116 kb on - strand, within Atu3059 at 60.122 kb on - strand, within Atu3059 at 60.122 kb on - strand, within Atu3059 at 60.124 kb on + strand, within Atu3059 at 60.124 kb on + strand, within Atu3059 at 60.124 kb on + strand, within Atu3059 at 60.159 kb on + strand, within Atu3059 at 60.180 kb on - strand, within Atu3059 at 60.182 kb on + strand, within Atu3059 at 60.204 kb on - strand, within Atu3059 at 60.214 kb on - strand, within Atu3059 at 60.278 kb on - strand at 60.306 kb on - strand at 60.306 kb on - strand at 60.307 kb on + strand at 60.325 kb on - strand at 60.389 kb on - strand at 60.470 kb on - strand at 60.508 kb on + strand at 60.508 kb on + strand at 60.532 kb on + strand at 60.540 kb on - strand at 60.540 kb on - strand at 60.540 kb on - strand at 60.540 kb on - strand at 60.574 kb on - strand at 60.610 kb on - strand at 60.622 kb on - strand at 60.738 kb on - strand at 60.783 kb on - strand at 60.828 kb on - strand at 60.847 kb on + strand at 60.902 kb on + strand, within Atu3060 at 60.910 kb on - strand, within Atu3060 at 60.986 kb on - strand, within Atu3060 at 60.988 kb on + strand, within Atu3060 at 61.078 kb on + strand, within Atu3060 at 61.086 kb on - strand, within Atu3060 at 61.147 kb on - strand, within Atu3060 at 61.229 kb on - strand, within Atu3060 at 61.343 kb on + strand, within Atu3060
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Carbenicillin 150 mM remove 57,885 - Atu3057 0.65 -0.2 58,063 + Atu3057 0.77 -0.7 58,101 - Atu3057 0.80 -3.7 58,133 - Atu3057 0.82 -0.5 58,139 - Atu3057 0.82 -1.0 58,146 + Atu3057 0.83 -0.8 58,146 + Atu3057 0.83 -0.2 58,146 + Atu3057 0.83 -0.7 58,187 + Atu3057 0.85 -1.7 58,195 - Atu3057 0.86 -0.0 58,195 - Atu3057 0.86 +0.1 58,257 + -0.0 58,334 + +0.2 58,342 - -0.1 58,403 - -0.6 58,440 + -1.1 58,448 - +0.0 58,546 + +0.6 58,636 + +0.0 58,751 + -0.1 58,807 + +0.6 58,815 - -0.5 58,819 + -0.1 58,819 + -1.3 58,889 - +2.7 58,889 - -0.1 58,906 - -0.6 58,913 + +0.6 58,926 + -0.7 58,935 + -0.1 59,037 + Atu3059 0.14 -0.4 59,096 + Atu3059 0.17 -0.1 59,106 + Atu3059 0.18 +0.3 59,106 + Atu3059 0.18 -1.1 59,106 + Atu3059 0.18 +0.7 59,114 - Atu3059 0.18 +0.2 59,114 - Atu3059 0.18 -1.5 59,157 + Atu3059 0.21 -0.7 59,165 - Atu3059 0.22 -0.3 59,224 + Atu3059 0.25 -0.3 59,227 - Atu3059 0.26 +0.4 59,244 + Atu3059 0.27 -0.5 59,490 + Atu3059 0.42 -0.1 59,490 + Atu3059 0.42 +0.3 59,490 + Atu3059 0.42 -0.1 59,490 + Atu3059 0.42 -0.4 59,490 + Atu3059 0.42 -0.2 59,490 + Atu3059 0.42 -0.4 59,490 + Atu3059 0.42 +0.5 59,490 + Atu3059 0.42 +0.3 59,490 + Atu3059 0.42 +0.4 59,493 - Atu3059 0.42 -0.9 59,498 - Atu3059 0.43 -0.5 59,507 + Atu3059 0.43 +0.1 59,516 + Atu3059 0.44 -0.0 59,520 + Atu3059 0.44 -0.0 59,528 + Atu3059 0.45 -1.4 59,536 - Atu3059 0.45 -0.4 59,548 - Atu3059 0.46 +0.0 59,581 - Atu3059 0.48 -0.6 59,632 + Atu3059 0.51 -0.8 59,632 + Atu3059 0.51 +0.0 59,635 - Atu3059 0.51 -0.8 59,640 - Atu3059 0.52 -2.0 59,640 - Atu3059 0.52 -2.0 59,650 - Atu3059 0.52 -1.0 59,711 + Atu3059 0.56 +0.0 59,764 - Atu3059 0.59 -0.8 59,844 + Atu3059 0.65 -0.4 59,851 - Atu3059 0.65 -0.8 59,852 - Atu3059 0.65 +0.6 59,852 - Atu3059 0.65 -0.8 59,868 + Atu3059 0.66 +0.0 59,880 + Atu3059 0.67 -1.1 59,880 + Atu3059 0.67 -0.1 59,898 - Atu3059 0.68 -0.7 59,915 + Atu3059 0.69 +0.5 59,972 - Atu3059 0.73 -0.6 59,972 - Atu3059 0.73 -0.6 60,006 + Atu3059 0.75 +0.2 60,022 - Atu3059 0.76 +0.2 60,041 + Atu3059 0.77 -0.9 60,049 - Atu3059 0.77 +0.7 60,050 + Atu3059 0.78 +0.2 60,116 - Atu3059 0.82 -0.4 60,122 - Atu3059 0.82 +0.3 60,122 - Atu3059 0.82 -0.5 60,124 + Atu3059 0.82 -0.3 60,124 + Atu3059 0.82 -0.5 60,124 + Atu3059 0.82 +1.2 60,159 + Atu3059 0.84 -0.8 60,180 - Atu3059 0.86 +0.0 60,182 + Atu3059 0.86 -0.1 60,204 - Atu3059 0.87 +0.6 60,214 - Atu3059 0.88 +0.4 60,278 - -1.8 60,306 - -1.1 60,306 - +0.1 60,307 + -0.1 60,325 - +0.1 60,389 - -0.6 60,470 - -0.2 60,508 + +0.2 60,508 + -1.7 60,532 + -0.5 60,540 - -0.5 60,540 - +1.0 60,540 - -0.7 60,540 - +0.1 60,574 - -0.7 60,610 - +0.9 60,622 - -0.5 60,738 - -1.9 60,783 - -0.5 60,828 - +0.7 60,847 + +0.0 60,902 + Atu3060 0.11 -0.7 60,910 - Atu3060 0.11 +0.4 60,986 - Atu3060 0.15 -0.0 60,988 + Atu3060 0.15 -2.2 61,078 + Atu3060 0.20 -0.6 61,086 - Atu3060 0.20 +0.1 61,147 - Atu3060 0.23 -0.7 61,229 - Atu3060 0.28 -0.1 61,343 + Atu3060 0.33 -0.6
Or see this region's nucleotide sequence