Experiment: LB_plus_SM_buffer with EIP16_phage 8.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF595 and GFF596 are separated by 3 nucleotides GFF596 and GFF597 are separated by 108 nucleotides GFF597 and GFF598 are separated by 2 nucleotides GFF598 and GFF599 are separated by 87 nucleotides
GFF595 - 'RNA-2',3'-PO4:RNA-5'-OH ligase' transl_table=11, at 330,499 to 331,716
GFF595
GFF596 - 'RNA 3'-terminal phosphate cyclase (EC 6.5.1.4)' transl_table=11, at 331,720 to 332,739
GFF596
GFF597 - DNA-damage-inducible protein J, at 332,848 to 333,108
GFF597
GFF598 - YafQ toxin protein, at 333,111 to 333,386
GFF598
GFF599 - Transcriptional activator of maltose regulon, MalT, at 333,474 to 336,179
GFF599
Position (kb) 331
332
333 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 330.809 kb on + strand, within GFF595 at 330.966 kb on + strand, within GFF595 at 330.970 kb on + strand, within GFF595 at 330.972 kb on + strand, within GFF595 at 331.093 kb on + strand, within GFF595 at 331.094 kb on - strand, within GFF595 at 331.163 kb on - strand, within GFF595 at 331.183 kb on + strand, within GFF595 at 331.336 kb on + strand, within GFF595 at 331.336 kb on + strand, within GFF595 at 331.336 kb on + strand, within GFF595 at 331.336 kb on + strand, within GFF595 at 331.337 kb on - strand, within GFF595 at 331.337 kb on - strand, within GFF595 at 331.372 kb on + strand, within GFF595 at 331.373 kb on - strand, within GFF595 at 331.373 kb on - strand, within GFF595 at 331.373 kb on - strand, within GFF595 at 331.629 kb on + strand at 331.681 kb on + strand at 331.681 kb on + strand at 331.682 kb on - strand at 331.982 kb on - strand, within GFF596 at 332.656 kb on - strand at 332.737 kb on + strand at 332.748 kb on + strand at 332.786 kb on - strand at 332.819 kb on + strand at 332.832 kb on + strand at 333.017 kb on + strand, within GFF597 at 333.018 kb on - strand, within GFF597 at 333.039 kb on + strand, within GFF597 at 333.179 kb on + strand, within GFF598 at 333.180 kb on - strand, within GFF598 at 333.180 kb on - strand, within GFF598 at 333.207 kb on - strand, within GFF598 at 333.251 kb on + strand, within GFF598 at 333.252 kb on - strand, within GFF598 at 333.320 kb on + strand, within GFF598 at 333.321 kb on - strand, within GFF598 at 333.326 kb on + strand, within GFF598 at 333.384 kb on + strand at 333.406 kb on - strand at 333.578 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 8.75 MOI remove 330,809 + GFF595 0.25 -0.5 330,966 + GFF595 0.38 -0.2 330,970 + GFF595 0.39 +2.9 330,972 + GFF595 0.39 +0.8 331,093 + GFF595 0.49 -0.9 331,094 - GFF595 0.49 +3.0 331,163 - GFF595 0.55 -0.4 331,183 + GFF595 0.56 -0.1 331,336 + GFF595 0.69 -0.4 331,336 + GFF595 0.69 -0.4 331,336 + GFF595 0.69 +2.9 331,336 + GFF595 0.69 +0.8 331,337 - GFF595 0.69 +0.2 331,337 - GFF595 0.69 -1.2 331,372 + GFF595 0.72 +0.4 331,373 - GFF595 0.72 +0.3 331,373 - GFF595 0.72 +0.1 331,373 - GFF595 0.72 -0.2 331,629 + -0.6 331,681 + -0.1 331,681 + +0.7 331,682 - -1.0 331,982 - GFF596 0.26 -0.4 332,656 - +0.3 332,737 + +1.1 332,748 + +1.4 332,786 - +0.5 332,819 + +0.2 332,832 + +0.0 333,017 + GFF597 0.65 -3.3 333,018 - GFF597 0.65 +0.5 333,039 + GFF597 0.73 -3.2 333,179 + GFF598 0.25 -0.3 333,180 - GFF598 0.25 +0.2 333,180 - GFF598 0.25 +0.7 333,207 - GFF598 0.35 -0.1 333,251 + GFF598 0.51 -0.2 333,252 - GFF598 0.51 +0.0 333,320 + GFF598 0.76 -0.8 333,321 - GFF598 0.76 -0.7 333,326 + GFF598 0.78 +0.9 333,384 + +0.1 333,406 - +0.5 333,578 + +0.9
Or see this region's nucleotide sequence