Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF55

Experiment: LB_plus_SM_buffer with EIP16_phage 8.75 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF54 and GFF55 are separated by 236 nucleotidesGFF55 and GFF56 are separated by 29 nucleotidesGFF56 and GFF57 are separated by 36 nucleotides GFF54 - Methionine aminopeptidase (EC 3.4.11.18), at 35,243 to 36,037 GFF54 GFF55 - [Protein-PII] uridylyltransferase (EC 2.7.7.59), at 36,274 to 38,946 GFF55 GFF56 - 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (EC 2.3.1.117), at 38,976 to 39,800 GFF56 GFF57 - Permeases of the major facilitator superfamily, at 39,837 to 41,138 GFF57 Position (kb) 36 37 38 39Strain fitness (log2 ratio) -1 0 1 2 3at 36.036 kb on - strandat 36.149 kb on + strandat 36.149 kb on + strandat 36.150 kb on - strandat 36.150 kb on - strandat 36.153 kb on + strandat 36.153 kb on + strandat 36.153 kb on + strandat 36.157 kb on + strandat 36.157 kb on + strandat 36.189 kb on + strandat 36.190 kb on - strandat 36.214 kb on - strandat 36.260 kb on - strandat 36.279 kb on + strandat 36.694 kb on - strand, within GFF55at 36.694 kb on - strand, within GFF55at 36.698 kb on + strand, within GFF55at 36.699 kb on - strand, within GFF55at 36.817 kb on - strand, within GFF55at 36.912 kb on + strand, within GFF55at 36.930 kb on + strand, within GFF55at 36.963 kb on + strand, within GFF55at 36.963 kb on + strand, within GFF55at 36.963 kb on + strand, within GFF55at 37.220 kb on - strand, within GFF55at 37.353 kb on + strand, within GFF55at 37.416 kb on + strand, within GFF55at 37.510 kb on + strand, within GFF55at 37.511 kb on - strand, within GFF55at 37.542 kb on + strand, within GFF55at 37.601 kb on + strand, within GFF55at 37.612 kb on + strand, within GFF55at 37.612 kb on + strand, within GFF55at 37.612 kb on + strand, within GFF55at 37.612 kb on + strand, within GFF55at 37.612 kb on + strand, within GFF55at 37.613 kb on - strand, within GFF55at 37.614 kb on + strand, within GFF55at 37.614 kb on + strand, within GFF55at 37.614 kb on + strand, within GFF55at 37.614 kb on + strand, within GFF55at 37.670 kb on - strand, within GFF55at 37.869 kb on - strand, within GFF55at 37.947 kb on - strand, within GFF55at 38.094 kb on + strand, within GFF55at 38.333 kb on + strand, within GFF55at 38.471 kb on + strand, within GFF55at 38.523 kb on + strand, within GFF55at 38.523 kb on + strand, within GFF55at 38.530 kb on - strand, within GFF55at 38.616 kb on + strand, within GFF55at 38.667 kb on + strand, within GFF55at 38.877 kb on + strandat 38.878 kb on - strandat 38.880 kb on + strandat 38.957 kb on + strandat 38.959 kb on + strandat 39.792 kb on - strandat 39.938 kb on + strandat 39.944 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 8.75 MOI
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36,036 - +1.2
36,149 + -0.3
36,149 + -0.3
36,150 - +1.5
36,150 - +0.7
36,153 + -0.0
36,153 + +0.6
36,153 + -0.2
36,157 + +0.3
36,157 + -1.7
36,189 + +0.5
36,190 - +2.4
36,214 - +2.4
36,260 - +3.0
36,279 + +1.3
36,694 - GFF55 0.16 +1.5
36,694 - GFF55 0.16 +2.7
36,698 + GFF55 0.16 +2.3
36,699 - GFF55 0.16 -0.1
36,817 - GFF55 0.20 +0.3
36,912 + GFF55 0.24 +2.4
36,930 + GFF55 0.25 +0.8
36,963 + GFF55 0.26 +1.7
36,963 + GFF55 0.26 +1.9
36,963 + GFF55 0.26 +1.8
37,220 - GFF55 0.35 +2.5
37,353 + GFF55 0.40 +3.2
37,416 + GFF55 0.43 +2.9
37,510 + GFF55 0.46 +3.5
37,511 - GFF55 0.46 +2.0
37,542 + GFF55 0.47 +1.4
37,601 + GFF55 0.50 +3.3
37,612 + GFF55 0.50 +1.3
37,612 + GFF55 0.50 +0.9
37,612 + GFF55 0.50 +0.8
37,612 + GFF55 0.50 +1.3
37,612 + GFF55 0.50 +0.3
37,613 - GFF55 0.50 +1.4
37,614 + GFF55 0.50 +0.6
37,614 + GFF55 0.50 +3.1
37,614 + GFF55 0.50 +2.2
37,614 + GFF55 0.50 +1.5
37,670 - GFF55 0.52 +1.6
37,869 - GFF55 0.60 +2.3
37,947 - GFF55 0.63 +3.1
38,094 + GFF55 0.68 +3.0
38,333 + GFF55 0.77 +2.1
38,471 + GFF55 0.82 +2.2
38,523 + GFF55 0.84 +2.2
38,523 + GFF55 0.84 +1.5
38,530 - GFF55 0.84 +3.0
38,616 + GFF55 0.88 +1.1
38,667 + GFF55 0.90 +3.7
38,877 + +1.3
38,878 - +1.6
38,880 + +2.7
38,957 + -0.7
38,959 + -0.4
39,792 - +0.3
39,938 + -0.3
39,944 - +0.4

Or see this region's nucleotide sequence