Experiment: LB_plus_SM_buffer with EIP16_phage 8.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF509 and GFF510 are separated by 63 nucleotides GFF510 and GFF511 are separated by 53 nucleotides GFF511 and GFF512 are separated by 58 nucleotides
GFF509 - Putative phosphosugar-binding protein, at 238,728 to 239,702
GFF509
GFF510 - kinase (ribokinase family), at 239,766 to 240,611
GFF510
GFF511 - anaerobic C4-dicarboxylate transporter, at 240,665 to 241,984
GFF511
GFF512 - L-asparaginase (EC 3.5.1.1), at 242,043 to 243,086
GFF512
Position (kb) 240
241
242 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 239.700 kb on + strand at 239.740 kb on - strand at 239.744 kb on + strand at 239.811 kb on + strand at 239.844 kb on - strand at 239.865 kb on + strand, within GFF510 at 239.936 kb on - strand, within GFF510 at 239.963 kb on - strand, within GFF510 at 240.418 kb on - strand, within GFF510 at 240.614 kb on + strand at 240.615 kb on - strand at 240.664 kb on + strand at 240.664 kb on + strand at 240.681 kb on + strand at 240.823 kb on + strand, within GFF511 at 240.856 kb on - strand, within GFF511 at 240.856 kb on - strand, within GFF511 at 240.923 kb on + strand, within GFF511 at 240.929 kb on - strand, within GFF511 at 240.929 kb on - strand, within GFF511 at 240.934 kb on - strand, within GFF511 at 240.953 kb on + strand, within GFF511 at 241.057 kb on - strand, within GFF511 at 241.103 kb on - strand, within GFF511 at 241.169 kb on - strand, within GFF511 at 241.229 kb on - strand, within GFF511 at 241.288 kb on - strand, within GFF511 at 241.507 kb on - strand, within GFF511 at 241.684 kb on + strand, within GFF511 at 241.685 kb on - strand, within GFF511 at 241.782 kb on + strand, within GFF511 at 241.880 kb on - strand at 241.889 kb on + strand at 242.096 kb on - strand at 242.169 kb on - strand, within GFF512 at 242.376 kb on - strand, within GFF512 at 242.410 kb on - strand, within GFF512 at 242.418 kb on - strand, within GFF512 at 242.609 kb on + strand, within GFF512 at 242.653 kb on - strand, within GFF512 at 242.734 kb on + strand, within GFF512 at 242.739 kb on - strand, within GFF512 at 242.739 kb on - strand, within GFF512 at 242.798 kb on + strand, within GFF512 at 242.798 kb on + strand, within GFF512 at 242.802 kb on + strand, within GFF512 at 242.802 kb on + strand, within GFF512 at 242.802 kb on + strand, within GFF512 at 242.802 kb on + strand, within GFF512 at 242.803 kb on - strand, within GFF512 at 242.838 kb on - strand, within GFF512 at 242.915 kb on + strand, within GFF512 at 242.954 kb on - strand, within GFF512 at 242.964 kb on - strand, within GFF512
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 8.75 MOI remove 239,700 + -0.3 239,740 - -1.2 239,744 + +0.4 239,811 + -1.6 239,844 - -0.1 239,865 + GFF510 0.12 +3.7 239,936 - GFF510 0.20 +0.8 239,963 - GFF510 0.23 +3.9 240,418 - GFF510 0.77 +0.3 240,614 + +0.4 240,615 - +0.3 240,664 + +3.1 240,664 + +1.1 240,681 + -0.9 240,823 + GFF511 0.12 +0.2 240,856 - GFF511 0.14 +0.2 240,856 - GFF511 0.14 +0.9 240,923 + GFF511 0.20 +0.4 240,929 - GFF511 0.20 +0.8 240,929 - GFF511 0.20 -0.9 240,934 - GFF511 0.20 +3.0 240,953 + GFF511 0.22 +0.6 241,057 - GFF511 0.30 +0.5 241,103 - GFF511 0.33 -0.7 241,169 - GFF511 0.38 +1.7 241,229 - GFF511 0.43 -0.6 241,288 - GFF511 0.47 -0.6 241,507 - GFF511 0.64 +0.2 241,684 + GFF511 0.77 +0.4 241,685 - GFF511 0.77 -0.3 241,782 + GFF511 0.85 -0.4 241,880 - -0.1 241,889 + -0.5 242,096 - -0.5 242,169 - GFF512 0.12 +0.8 242,376 - GFF512 0.32 -0.2 242,410 - GFF512 0.35 -0.4 242,418 - GFF512 0.36 +0.2 242,609 + GFF512 0.54 +3.0 242,653 - GFF512 0.58 +3.1 242,734 + GFF512 0.66 -1.2 242,739 - GFF512 0.67 +0.3 242,739 - GFF512 0.67 -0.2 242,798 + GFF512 0.72 +1.2 242,798 + GFF512 0.72 +0.9 242,802 + GFF512 0.73 +2.4 242,802 + GFF512 0.73 +1.2 242,802 + GFF512 0.73 +2.8 242,802 + GFF512 0.73 +0.6 242,803 - GFF512 0.73 -0.2 242,838 - GFF512 0.76 +0.2 242,915 + GFF512 0.84 -2.2 242,954 - GFF512 0.87 +0.2 242,964 - GFF512 0.88 +1.6
Or see this region's nucleotide sequence