Experiment: LB_plus_SM_buffer with EIP16_phage 8.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF495 and GFF496 are separated by 261 nucleotides GFF496 and GFF497 are separated by 53 nucleotides
GFF495 - Aerobic C4-dicarboxylate transporter for fumarate, L-malate, D-malate, succunate, at 221,522 to 222,808
GFF495
GFF496 - Protein YhjJ, putative peptidase, at 223,070 to 224,515
GFF496
GFF497 - 2-dehydro-3-deoxygluconate kinase (EC 2.7.1.45), at 224,569 to 225,498
GFF497
Position (kb) 223
224
225 Strain fitness (log2 ratio) -1
0
1
2 at 222.153 kb on - strand, within GFF495 at 222.153 kb on - strand, within GFF495 at 222.191 kb on - strand, within GFF495 at 222.200 kb on + strand, within GFF495 at 222.201 kb on - strand, within GFF495 at 222.201 kb on - strand, within GFF495 at 222.201 kb on - strand, within GFF495 at 222.202 kb on + strand, within GFF495 at 222.212 kb on - strand, within GFF495 at 222.284 kb on + strand, within GFF495 at 222.285 kb on - strand, within GFF495 at 222.440 kb on - strand, within GFF495 at 222.490 kb on + strand, within GFF495 at 222.510 kb on + strand, within GFF495 at 222.510 kb on + strand, within GFF495 at 222.592 kb on + strand, within GFF495 at 222.655 kb on + strand, within GFF495 at 222.655 kb on + strand, within GFF495 at 222.736 kb on - strand at 222.764 kb on - strand at 222.819 kb on + strand at 222.819 kb on + strand at 222.860 kb on - strand at 222.860 kb on - strand at 222.883 kb on + strand at 222.883 kb on + strand at 222.883 kb on + strand at 222.883 kb on + strand at 222.884 kb on - strand at 222.884 kb on - strand at 222.897 kb on + strand at 222.961 kb on - strand at 222.967 kb on + strand at 223.089 kb on - strand at 223.089 kb on - strand at 223.196 kb on - strand at 223.216 kb on + strand, within GFF496 at 223.395 kb on - strand, within GFF496 at 223.449 kb on - strand, within GFF496 at 223.468 kb on + strand, within GFF496 at 223.519 kb on + strand, within GFF496 at 223.520 kb on - strand, within GFF496 at 223.546 kb on + strand, within GFF496 at 223.547 kb on - strand, within GFF496 at 223.822 kb on + strand, within GFF496 at 223.823 kb on - strand, within GFF496 at 223.858 kb on + strand, within GFF496 at 223.859 kb on - strand, within GFF496 at 224.056 kb on + strand, within GFF496 at 224.056 kb on + strand, within GFF496 at 224.057 kb on - strand, within GFF496 at 224.728 kb on + strand, within GFF497 at 224.818 kb on + strand, within GFF497 at 224.949 kb on - strand, within GFF497 at 224.998 kb on - strand, within GFF497 at 224.998 kb on - strand, within GFF497 at 224.998 kb on - strand, within GFF497 at 225.090 kb on + strand, within GFF497 at 225.110 kb on + strand, within GFF497 at 225.110 kb on + strand, within GFF497 at 225.197 kb on + strand, within GFF497 at 225.234 kb on - strand, within GFF497 at 225.234 kb on - strand, within GFF497 at 225.236 kb on + strand, within GFF497 at 225.236 kb on + strand, within GFF497 at 225.237 kb on - strand, within GFF497 at 225.280 kb on - strand, within GFF497 at 225.350 kb on + strand, within GFF497 at 225.351 kb on - strand, within GFF497 at 225.390 kb on + strand, within GFF497 at 225.390 kb on + strand, within GFF497 at 225.392 kb on + strand, within GFF497 at 225.392 kb on + strand, within GFF497 at 225.403 kb on - strand, within GFF497 at 225.403 kb on - strand, within GFF497 at 225.403 kb on - strand, within GFF497 at 225.498 kb on + strand at 225.499 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 8.75 MOI remove 222,153 - GFF495 0.49 -0.0 222,153 - GFF495 0.49 -0.3 222,191 - GFF495 0.52 +0.9 222,200 + GFF495 0.53 -0.6 222,201 - GFF495 0.53 +0.0 222,201 - GFF495 0.53 +1.6 222,201 - GFF495 0.53 -0.5 222,202 + GFF495 0.53 +1.4 222,212 - GFF495 0.54 -1.2 222,284 + GFF495 0.59 -0.3 222,285 - GFF495 0.59 -0.2 222,440 - GFF495 0.71 +0.1 222,490 + GFF495 0.75 +0.4 222,510 + GFF495 0.77 -0.6 222,510 + GFF495 0.77 -0.8 222,592 + GFF495 0.83 -1.0 222,655 + GFF495 0.88 -0.7 222,655 + GFF495 0.88 -0.8 222,736 - -0.2 222,764 - -0.5 222,819 + +0.0 222,819 + -0.6 222,860 - +0.0 222,860 - -0.0 222,883 + +0.6 222,883 + -1.2 222,883 + +0.7 222,883 + -0.4 222,884 - -0.0 222,884 - -0.3 222,897 + -0.0 222,961 - +0.0 222,967 + +0.4 223,089 - -0.6 223,089 - -0.2 223,196 - +0.0 223,216 + GFF496 0.10 +0.0 223,395 - GFF496 0.22 -1.6 223,449 - GFF496 0.26 -0.1 223,468 + GFF496 0.28 +0.2 223,519 + GFF496 0.31 +0.6 223,520 - GFF496 0.31 -1.4 223,546 + GFF496 0.33 +0.2 223,547 - GFF496 0.33 -0.3 223,822 + GFF496 0.52 +0.3 223,823 - GFF496 0.52 +0.1 223,858 + GFF496 0.54 -0.9 223,859 - GFF496 0.55 +0.4 224,056 + GFF496 0.68 -0.2 224,056 + GFF496 0.68 +0.2 224,057 - GFF496 0.68 -0.1 224,728 + GFF497 0.17 -0.2 224,818 + GFF497 0.27 +0.2 224,949 - GFF497 0.41 -0.9 224,998 - GFF497 0.46 +0.7 224,998 - GFF497 0.46 +0.1 224,998 - GFF497 0.46 -0.2 225,090 + GFF497 0.56 -0.3 225,110 + GFF497 0.58 +0.2 225,110 + GFF497 0.58 -0.4 225,197 + GFF497 0.68 -0.4 225,234 - GFF497 0.72 +0.0 225,234 - GFF497 0.72 +2.0 225,236 + GFF497 0.72 -0.7 225,236 + GFF497 0.72 -0.2 225,237 - GFF497 0.72 +0.7 225,280 - GFF497 0.76 +2.5 225,350 + GFF497 0.84 +0.3 225,351 - GFF497 0.84 +0.0 225,390 + GFF497 0.88 -0.3 225,390 + GFF497 0.88 -1.1 225,392 + GFF497 0.88 -0.0 225,392 + GFF497 0.88 +0.6 225,403 - GFF497 0.90 +0.5 225,403 - GFF497 0.90 -0.7 225,403 - GFF497 0.90 -0.3 225,498 + +0.2 225,499 - -0.1
Or see this region's nucleotide sequence