Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF4874

Experiment: LB_plus_SM_buffer with EIP16_phage 8.75 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4872 and GFF4873 are separated by 62 nucleotidesGFF4873 and GFF4874 are separated by 3 nucleotidesGFF4874 and GFF4690 are separated by 303 nucleotidesGFF4690 and GFF4691 are separated by 234 nucleotides GFF4872 - tRNA-Arg-ACG, at 116 to 192 GFF4872 GFF4873 - tRNA-Arg-ACG, at 255 to 331 GFF4873 GFF4874 - tRNA-Ser-GCT, at 335 to 427 GFF4874 GFF4690 - Carbon storage regulator, at 731 to 916 GFF4690 GFF4691 - Alanyl-tRNA synthetase (EC 6.1.1.7), at 1,151 to 3,781 GFF4691 Position (kb) 0 1Strain fitness (log2 ratio) -1 0 1 2 3 4at 0.249 kb on - strandat 0.439 kb on - strandat 0.561 kb on + strandat 0.561 kb on + strandat 0.732 kb on + strandat 0.733 kb on - strandat 0.977 kb on + strandat 0.977 kb on + strandat 0.977 kb on + strandat 1.011 kb on + strandat 1.012 kb on - strandat 1.033 kb on + strandat 1.033 kb on + strandat 1.085 kb on + strandat 1.149 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 8.75 MOI
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249 - +2.2
439 - +0.4
561 + +0.4
561 + +0.4
732 + +2.1
733 - +1.4
977 + +2.9
977 + +4.0
977 + +2.2
1,011 + +2.2
1,012 - +1.7
1,033 + +3.1
1,033 + +2.6
1,085 + +1.7
1,149 - +1.8

Or see this region's nucleotide sequence