Experiment: LB_plus_SM_buffer with EIP16_phage 8.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4783 and GFF4784 are separated by 18 nucleotides GFF4784 and GFF4785 are separated by 170 nucleotides GFF4785 and GFF4786 overlap by 1 nucleotides
GFF4783 - DeoR family transcriptional regulator probably related to glycerate, glycolaldehyde metabolism, at 91,607 to 92,371
GFF4783
GFF4784 - Transcriptional regulator, MarR family, at 92,390 to 92,794
GFF4784
GFF4785 - 3-polyprenyl-4-hydroxybenzoate carboxy-lyase UbiX (EC 4.1.1.-), at 92,965 to 93,558
GFF4785
GFF4786 - UbiD family decarboxylase, MJ1133 type, at 93,558 to 94,985
GFF4786
Position (kb)
92
93
94 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 92.020 kb on + strand, within GFF4783 at 92.237 kb on + strand, within GFF4783 at 92.344 kb on + strand at 92.409 kb on - strand at 92.527 kb on - strand, within GFF4784 at 92.527 kb on - strand, within GFF4784 at 92.787 kb on - strand at 92.827 kb on + strand at 92.850 kb on - strand at 92.942 kb on - strand at 93.143 kb on - strand, within GFF4785 at 93.143 kb on - strand, within GFF4785 at 93.210 kb on + strand, within GFF4785 at 93.210 kb on + strand, within GFF4785 at 93.211 kb on - strand, within GFF4785 at 93.211 kb on - strand, within GFF4785 at 93.211 kb on - strand, within GFF4785 at 93.225 kb on + strand, within GFF4785 at 93.225 kb on + strand, within GFF4785 at 93.226 kb on - strand, within GFF4785 at 93.444 kb on + strand, within GFF4785 at 93.495 kb on + strand, within GFF4785 at 93.495 kb on + strand, within GFF4785 at 93.729 kb on - strand, within GFF4786 at 93.729 kb on - strand, within GFF4786 at 93.753 kb on - strand, within GFF4786 at 93.899 kb on + strand, within GFF4786 at 93.975 kb on + strand, within GFF4786 at 93.976 kb on - strand, within GFF4786 at 94.051 kb on - strand, within GFF4786 at 94.076 kb on + strand, within GFF4786 at 94.076 kb on + strand, within GFF4786 at 94.191 kb on - strand, within GFF4786 at 94.271 kb on + strand, within GFF4786 at 94.350 kb on - strand, within GFF4786
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 8.75 MOI remove 92,020 + GFF4783 0.54 +1.8 92,237 + GFF4783 0.82 -0.1 92,344 + -0.4 92,409 - -1.0 92,527 - GFF4784 0.34 -0.3 92,527 - GFF4784 0.34 -0.4 92,787 - -1.3 92,827 + -0.5 92,850 - -0.1 92,942 - +0.3 93,143 - GFF4785 0.30 +1.0 93,143 - GFF4785 0.30 -0.5 93,210 + GFF4785 0.41 -0.6 93,210 + GFF4785 0.41 +1.3 93,211 - GFF4785 0.41 -0.5 93,211 - GFF4785 0.41 -0.3 93,211 - GFF4785 0.41 -0.8 93,225 + GFF4785 0.44 -0.3 93,225 + GFF4785 0.44 -0.2 93,226 - GFF4785 0.44 +0.4 93,444 + GFF4785 0.81 -1.9 93,495 + GFF4785 0.89 -1.1 93,495 + GFF4785 0.89 +0.5 93,729 - GFF4786 0.12 +0.2 93,729 - GFF4786 0.12 -0.3 93,753 - GFF4786 0.14 +1.1 93,899 + GFF4786 0.24 -0.8 93,975 + GFF4786 0.29 -1.5 93,976 - GFF4786 0.29 -0.1 94,051 - GFF4786 0.35 -2.0 94,076 + GFF4786 0.36 -1.3 94,076 + GFF4786 0.36 -2.4 94,191 - GFF4786 0.44 -0.1 94,271 + GFF4786 0.50 -1.1 94,350 - GFF4786 0.55 +0.3
Or see this region's nucleotide sequence