Experiment: LB_plus_SM_buffer with EIP16_phage 8.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4695 and GFF4696 are separated by 253 nucleotides GFF4696 and GFF4697 overlap by 4 nucleotides GFF4697 and GFF4698 are separated by 11 nucleotides GFF4698 and GFF4699 are separated by 11 nucleotides
GFF4695 - Membrane-bound lytic murein transglycosylase B precursor (EC 3.2.1.-), at 6,755 to 7,834
GFF4695
GFF4696 - PTS system, glucitol/sorbitol-specific IIC component (EC 2.7.1.69), at 8,088 to 8,651
GFF4696
GFF4697 - PTS system, glucitol/sorbitol-specific IIB component and second of two IIC components (EC 2.7.1.69), at 8,648 to 9,619
GFF4697
GFF4698 - PTS system, glucitol/sorbitol-specific IIA component (EC 2.7.1.69), at 9,631 to 9,993
GFF4698
GFF4699 - Sorbitol-6-phosphate 2-dehydrogenase (EC 1.1.1.140), at 10,005 to 10,784
GFF4699
Position (kb)
8
9
10 Strain fitness (log2 ratio)
-2
-1
0
1 at 7.699 kb on + strand, within GFF4695 at 7.876 kb on - strand at 7.915 kb on + strand at 8.102 kb on + strand at 8.174 kb on + strand, within GFF4696 at 8.289 kb on + strand, within GFF4696 at 8.290 kb on - strand, within GFF4696 at 8.299 kb on + strand, within GFF4696 at 8.383 kb on - strand, within GFF4696 at 8.386 kb on - strand, within GFF4696 at 8.400 kb on + strand, within GFF4696 at 8.435 kb on + strand, within GFF4696 at 8.435 kb on + strand, within GFF4696 at 8.524 kb on - strand, within GFF4696 at 8.534 kb on - strand, within GFF4696 at 8.534 kb on - strand, within GFF4696 at 8.711 kb on - strand at 8.732 kb on + strand at 8.732 kb on + strand at 8.734 kb on + strand at 8.734 kb on + strand at 8.735 kb on - strand at 8.735 kb on - strand at 8.781 kb on + strand, within GFF4697 at 8.951 kb on + strand, within GFF4697 at 8.954 kb on - strand, within GFF4697 at 8.970 kb on - strand, within GFF4697 at 8.998 kb on + strand, within GFF4697 at 9.142 kb on - strand, within GFF4697 at 9.233 kb on - strand, within GFF4697 at 9.233 kb on - strand, within GFF4697 at 9.579 kb on + strand at 9.580 kb on - strand at 9.608 kb on - strand at 9.618 kb on - strand at 9.709 kb on - strand, within GFF4698 at 9.709 kb on - strand, within GFF4698 at 9.880 kb on - strand, within GFF4698 at 9.929 kb on + strand, within GFF4698 at 10.104 kb on - strand, within GFF4699 at 10.264 kb on - strand, within GFF4699 at 10.428 kb on - strand, within GFF4699 at 10.540 kb on + strand, within GFF4699
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 8.75 MOI remove 7,699 + GFF4695 0.87 -0.6 7,876 - -0.7 7,915 + -1.9 8,102 + +0.6 8,174 + GFF4696 0.15 -0.3 8,289 + GFF4696 0.36 +0.1 8,290 - GFF4696 0.36 +0.5 8,299 + GFF4696 0.37 -0.1 8,383 - GFF4696 0.52 +0.8 8,386 - GFF4696 0.53 +0.3 8,400 + GFF4696 0.55 +0.1 8,435 + GFF4696 0.62 -0.3 8,435 + GFF4696 0.62 +0.7 8,524 - GFF4696 0.77 -0.0 8,534 - GFF4696 0.79 +0.1 8,534 - GFF4696 0.79 +1.3 8,711 - -0.4 8,732 + -0.5 8,732 + +0.8 8,734 + +0.9 8,734 + -0.0 8,735 - +0.0 8,735 - -0.8 8,781 + GFF4697 0.14 -0.9 8,951 + GFF4697 0.31 +0.4 8,954 - GFF4697 0.31 -0.7 8,970 - GFF4697 0.33 +0.5 8,998 + GFF4697 0.36 +0.2 9,142 - GFF4697 0.51 +1.1 9,233 - GFF4697 0.60 -0.3 9,233 - GFF4697 0.60 +1.7 9,579 + -0.3 9,580 - +0.7 9,608 - +0.9 9,618 - +0.2 9,709 - GFF4698 0.21 -0.1 9,709 - GFF4698 0.21 +1.2 9,880 - GFF4698 0.69 +0.4 9,929 + GFF4698 0.82 -0.8 10,104 - GFF4699 0.13 +0.7 10,264 - GFF4699 0.33 +1.0 10,428 - GFF4699 0.54 +0.6 10,540 + GFF4699 0.69 +0.2
Or see this region's nucleotide sequence