Experiment: LB_plus_SM_buffer with EIP16_phage 8.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4493 and GFF4494 are separated by 249 nucleotides GFF4494 and GFF4495 are separated by 52 nucleotides GFF4495 and GFF4496 overlap by 4 nucleotides
GFF4493 - Enterobactin synthetase component F, serine activating enzyme (EC 2.7.7.-), at 275,676 to 279,560
GFF4493
GFF4494 - Ferric enterobactin uptake protein FepE, at 279,810 to 280,946
GFF4494
GFF4495 - ABC-type Fe3+-siderophore transport system, ATPase component, at 280,999 to 281,793
GFF4495
GFF4496 - Ferric enterobactin transport system permease protein FepG (TC 3.A.1.14.2), at 281,790 to 282,779
GFF4496
Position (kb) 279
280
281 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 278.881 kb on + strand, within GFF4493 at 278.882 kb on - strand, within GFF4493 at 278.926 kb on + strand, within GFF4493 at 279.022 kb on + strand, within GFF4493 at 279.076 kb on - strand, within GFF4493 at 279.076 kb on - strand, within GFF4493 at 279.079 kb on + strand, within GFF4493 at 279.082 kb on + strand, within GFF4493 at 279.236 kb on - strand at 279.386 kb on + strand at 279.386 kb on + strand at 279.387 kb on - strand at 279.494 kb on - strand at 279.658 kb on + strand at 279.906 kb on - strand at 280.281 kb on - strand, within GFF4494 at 280.386 kb on + strand, within GFF4494 at 280.387 kb on - strand, within GFF4494 at 280.387 kb on - strand, within GFF4494 at 280.562 kb on + strand, within GFF4494 at 280.575 kb on + strand, within GFF4494 at 280.880 kb on - strand at 280.928 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 8.75 MOI remove 278,881 + GFF4493 0.82 +0.2 278,882 - GFF4493 0.83 +0.3 278,926 + GFF4493 0.84 +0.0 279,022 + GFF4493 0.86 -0.4 279,076 - GFF4493 0.88 +2.6 279,076 - GFF4493 0.88 -3.2 279,079 + GFF4493 0.88 +1.2 279,082 + GFF4493 0.88 -0.1 279,236 - -0.1 279,386 + -0.2 279,386 + -0.5 279,387 - -0.1 279,494 - -0.4 279,658 + -2.6 279,906 - -1.0 280,281 - GFF4494 0.41 +0.6 280,386 + GFF4494 0.51 +0.3 280,387 - GFF4494 0.51 +0.0 280,387 - GFF4494 0.51 +0.3 280,562 + GFF4494 0.66 +2.7 280,575 + GFF4494 0.67 -0.0 280,880 - +0.6 280,928 - -0.1
Or see this region's nucleotide sequence