Experiment: LB_plus_SM_buffer with EIP16_phage 8.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4462 and GFF4658 are separated by 348 nucleotides GFF4658 and GFF4463 are separated by 163 nucleotides GFF4463 and GFF4464 are separated by 238 nucleotides GFF4464 and GFF4465 are separated by 108 nucleotides GFF4465 and GFF4466 are separated by 50 nucleotides
GFF4462 - Bactoprenol-linked glucose translocase, at 247,574 to 247,849
GFF4462
GFF4658 - tRNA-Pseudo-TCT, at 248,198 to 248,270
GFF4658
GFF4463 - copE1, at 248,434 to 248,565
GFF4463
GFF4464 - sensor histidine kinase, at 248,804 to 249,133
GFF4464
GFF4465 - Cobalt-zinc-cadmium resistance protein CzcA; Cation efflux system protein CusA, at 249,242 to 249,421
GFF4465
GFF4466 - Putative HTH-type transcriptional regulator ykgD, at 249,472 to 250,326
GFF4466
Position (kb) 248
249
250 Strain fitness (log2 ratio) -2
-1
0
1
2 at 247.843 kb on + strand at 248.043 kb on - strand at 248.348 kb on + strand at 248.349 kb on - strand at 248.349 kb on - strand at 248.361 kb on - strand at 248.661 kb on - strand at 248.661 kb on - strand at 248.667 kb on - strand at 248.842 kb on + strand, within GFF4464 at 248.915 kb on + strand, within GFF4464 at 249.028 kb on + strand, within GFF4464 at 249.028 kb on + strand, within GFF4464 at 249.085 kb on + strand, within GFF4464 at 249.112 kb on + strand at 249.289 kb on - strand, within GFF4465 at 249.377 kb on + strand, within GFF4465 at 249.566 kb on + strand, within GFF4466 at 249.670 kb on - strand, within GFF4466 at 249.868 kb on - strand, within GFF4466 at 249.895 kb on + strand, within GFF4466 at 250.069 kb on - strand, within GFF4466 at 250.069 kb on - strand, within GFF4466
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 8.75 MOI remove 247,843 + -1.4 248,043 - +0.7 248,348 + -0.1 248,349 - +0.6 248,349 - -0.1 248,361 - +0.2 248,661 - -1.1 248,661 - -0.3 248,667 - +0.1 248,842 + GFF4464 0.12 -2.2 248,915 + GFF4464 0.34 +2.7 249,028 + GFF4464 0.68 +0.1 249,028 + GFF4464 0.68 -0.6 249,085 + GFF4464 0.85 -1.1 249,112 + -0.2 249,289 - GFF4465 0.26 +0.2 249,377 + GFF4465 0.75 +0.1 249,566 + GFF4466 0.11 +0.7 249,670 - GFF4466 0.23 +1.8 249,868 - GFF4466 0.46 +1.0 249,895 + GFF4466 0.49 +1.1 250,069 - GFF4466 0.70 -0.1 250,069 - GFF4466 0.70 +2.0
Or see this region's nucleotide sequence