Experiment: LB_plus_SM_buffer with EIP16_phage 8.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4365 and GFF4366 overlap by 8 nucleotides GFF4366 and GFF4367 are separated by 212 nucleotides GFF4367 and GFF4368 are separated by 31 nucleotides
GFF4365 - ATP-binding component, at 146,979 to 148,751
GFF4365
GFF4366 - putative multidrug transporter membrane ATP-binding components, at 148,744 to 150,525
GFF4366
GFF4367 - Nitrogen regulatory protein P-II, glnK, at 150,738 to 151,076
GFF4367
GFF4368 - Ammonium transporter, at 151,108 to 152,394
GFF4368
Position (kb) 148
149
150
151 Strain fitness (log2 ratio) -1
0
1
2
3 at 147.819 kb on - strand, within GFF4365 at 147.826 kb on - strand, within GFF4365 at 147.942 kb on - strand, within GFF4365 at 148.197 kb on - strand, within GFF4365 at 148.254 kb on - strand, within GFF4365 at 148.330 kb on + strand, within GFF4365 at 148.330 kb on + strand, within GFF4365 at 148.330 kb on + strand, within GFF4365 at 148.439 kb on - strand, within GFF4365 at 148.676 kb on + strand at 148.680 kb on + strand at 148.680 kb on + strand at 148.680 kb on + strand at 148.681 kb on - strand at 148.763 kb on + strand at 148.764 kb on - strand at 148.764 kb on - strand at 148.955 kb on - strand, within GFF4366 at 148.965 kb on - strand, within GFF4366 at 149.006 kb on + strand, within GFF4366 at 149.158 kb on + strand, within GFF4366 at 149.158 kb on + strand, within GFF4366 at 149.159 kb on - strand, within GFF4366 at 149.183 kb on + strand, within GFF4366 at 149.183 kb on + strand, within GFF4366 at 149.183 kb on + strand, within GFF4366 at 149.277 kb on - strand, within GFF4366 at 149.296 kb on + strand, within GFF4366 at 149.321 kb on + strand, within GFF4366 at 149.322 kb on - strand, within GFF4366 at 149.338 kb on + strand, within GFF4366 at 149.349 kb on + strand, within GFF4366 at 149.391 kb on - strand, within GFF4366 at 149.456 kb on - strand, within GFF4366 at 149.535 kb on - strand, within GFF4366 at 149.562 kb on + strand, within GFF4366 at 149.596 kb on + strand, within GFF4366 at 149.607 kb on + strand, within GFF4366 at 149.607 kb on + strand, within GFF4366 at 149.611 kb on + strand, within GFF4366 at 149.611 kb on + strand, within GFF4366 at 149.666 kb on + strand, within GFF4366 at 149.769 kb on + strand, within GFF4366 at 149.770 kb on - strand, within GFF4366 at 149.828 kb on + strand, within GFF4366 at 149.912 kb on - strand, within GFF4366 at 149.912 kb on - strand, within GFF4366 at 149.912 kb on - strand, within GFF4366 at 150.145 kb on - strand, within GFF4366 at 150.234 kb on - strand, within GFF4366 at 150.615 kb on + strand at 150.616 kb on - strand at 150.616 kb on - strand at 150.717 kb on + strand at 150.737 kb on + strand at 150.738 kb on - strand at 150.738 kb on - strand at 150.888 kb on + strand, within GFF4367 at 150.982 kb on - strand, within GFF4367 at 150.982 kb on - strand, within GFF4367 at 151.341 kb on + strand, within GFF4368 at 151.342 kb on - strand, within GFF4368 at 151.459 kb on + strand, within GFF4368 at 151.467 kb on + strand, within GFF4368
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 8.75 MOI remove 147,819 - GFF4365 0.47 +0.5 147,826 - GFF4365 0.48 +1.1 147,942 - GFF4365 0.54 +0.9 148,197 - GFF4365 0.69 +0.2 148,254 - GFF4365 0.72 +1.0 148,330 + GFF4365 0.76 +0.4 148,330 + GFF4365 0.76 +0.9 148,330 + GFF4365 0.76 +1.4 148,439 - GFF4365 0.82 +0.8 148,676 + +0.2 148,680 + +2.5 148,680 + +1.0 148,680 + +0.1 148,681 - +2.1 148,763 + +1.8 148,764 - +0.5 148,764 - +0.7 148,955 - GFF4366 0.12 +1.7 148,965 - GFF4366 0.12 +0.6 149,006 + GFF4366 0.15 +1.1 149,158 + GFF4366 0.23 +1.6 149,158 + GFF4366 0.23 +0.3 149,159 - GFF4366 0.23 +0.9 149,183 + GFF4366 0.25 +1.9 149,183 + GFF4366 0.25 +0.7 149,183 + GFF4366 0.25 +2.9 149,277 - GFF4366 0.30 +0.9 149,296 + GFF4366 0.31 +0.6 149,321 + GFF4366 0.32 +0.9 149,322 - GFF4366 0.32 +0.5 149,338 + GFF4366 0.33 +0.8 149,349 + GFF4366 0.34 +3.6 149,391 - GFF4366 0.36 +1.0 149,456 - GFF4366 0.40 +1.7 149,535 - GFF4366 0.44 +1.7 149,562 + GFF4366 0.46 +1.0 149,596 + GFF4366 0.48 +1.5 149,607 + GFF4366 0.48 +1.1 149,607 + GFF4366 0.48 +0.3 149,611 + GFF4366 0.49 +1.3 149,611 + GFF4366 0.49 +1.0 149,666 + GFF4366 0.52 +0.9 149,769 + GFF4366 0.58 +0.2 149,770 - GFF4366 0.58 +1.6 149,828 + GFF4366 0.61 +0.2 149,912 - GFF4366 0.66 +0.8 149,912 - GFF4366 0.66 +1.3 149,912 - GFF4366 0.66 +1.0 150,145 - GFF4366 0.79 +0.1 150,234 - GFF4366 0.84 +1.3 150,615 + +0.3 150,616 - +0.4 150,616 - -1.0 150,717 + -0.2 150,737 + +2.2 150,738 - -0.5 150,738 - +1.1 150,888 + GFF4367 0.44 -0.2 150,982 - GFF4367 0.72 +0.4 150,982 - GFF4367 0.72 +0.2 151,341 + GFF4368 0.18 +0.7 151,342 - GFF4368 0.18 +0.9 151,459 + GFF4368 0.27 -0.4 151,467 + GFF4368 0.28 +1.0
Or see this region's nucleotide sequence