Experiment: LB_plus_SM_buffer with EIP16_phage 8.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4286 and GFF4287 are separated by 26 nucleotides GFF4287 and GFF4288 are separated by 250 nucleotides GFF4288 and GFF4289 are separated by 70 nucleotides GFF4289 and GFF4290 are separated by 37 nucleotides
GFF4286 - Shikimate kinase III (EC 2.7.1.71), at 71,057 to 71,602
GFF4286
GFF4287 - Protein YaiA, at 71,629 to 71,820
GFF4287
GFF4288 - AroM protein, at 72,071 to 72,748
GFF4288
GFF4289 - Cytoplasmic protein YaiE, at 72,819 to 73,103
GFF4289
GFF4290 - DNA recombination-dependent growth factor C, at 73,141 to 74,052
GFF4290
Position (kb) 72
73 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 71.072 kb on + strand at 71.072 kb on + strand at 71.297 kb on - strand, within GFF4286 at 71.299 kb on + strand, within GFF4286 at 71.312 kb on + strand, within GFF4286 at 71.315 kb on - strand, within GFF4286 at 71.315 kb on - strand, within GFF4286 at 71.325 kb on - strand, within GFF4286 at 71.326 kb on + strand, within GFF4286 at 71.326 kb on + strand, within GFF4286 at 71.327 kb on - strand, within GFF4286 at 71.327 kb on - strand, within GFF4286 at 71.327 kb on - strand, within GFF4286 at 71.327 kb on - strand, within GFF4286 at 71.327 kb on - strand, within GFF4286 at 71.351 kb on + strand, within GFF4286 at 71.352 kb on - strand, within GFF4286 at 71.502 kb on - strand, within GFF4286 at 71.596 kb on + strand at 71.601 kb on - strand at 71.662 kb on + strand, within GFF4287 at 71.890 kb on + strand at 71.933 kb on + strand at 71.933 kb on + strand at 71.962 kb on + strand at 71.963 kb on - strand at 72.054 kb on - strand at 72.127 kb on - strand at 72.172 kb on + strand, within GFF4288 at 72.352 kb on + strand, within GFF4288 at 72.355 kb on - strand, within GFF4288 at 72.398 kb on + strand, within GFF4288 at 72.421 kb on + strand, within GFF4288 at 72.582 kb on + strand, within GFF4288 at 72.707 kb on - strand at 72.780 kb on - strand at 72.780 kb on - strand at 72.854 kb on + strand, within GFF4289 at 72.855 kb on - strand, within GFF4289 at 72.921 kb on + strand, within GFF4289 at 72.922 kb on - strand, within GFF4289 at 72.924 kb on - strand, within GFF4289 at 72.924 kb on - strand, within GFF4289 at 73.014 kb on - strand, within GFF4289 at 73.083 kb on + strand at 73.084 kb on - strand at 73.257 kb on + strand, within GFF4290 at 73.374 kb on + strand, within GFF4290 at 73.605 kb on + strand, within GFF4290 at 73.605 kb on + strand, within GFF4290
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 8.75 MOI remove 71,072 + -1.5 71,072 + -4.7 71,297 - GFF4286 0.44 -1.0 71,299 + GFF4286 0.44 -2.3 71,312 + GFF4286 0.47 -2.3 71,315 - GFF4286 0.47 +0.9 71,315 - GFF4286 0.47 -0.1 71,325 - GFF4286 0.49 -0.4 71,326 + GFF4286 0.49 -3.6 71,326 + GFF4286 0.49 -0.9 71,327 - GFF4286 0.49 -0.3 71,327 - GFF4286 0.49 -0.9 71,327 - GFF4286 0.49 +1.1 71,327 - GFF4286 0.49 -0.7 71,327 - GFF4286 0.49 -0.0 71,351 + GFF4286 0.54 -2.8 71,352 - GFF4286 0.54 -0.8 71,502 - GFF4286 0.82 +0.3 71,596 + -1.3 71,601 - +0.7 71,662 + GFF4287 0.17 -0.4 71,890 + -2.1 71,933 + -2.8 71,933 + -1.7 71,962 + -2.4 71,963 - +1.3 72,054 - +2.0 72,127 - +0.8 72,172 + GFF4288 0.15 +1.3 72,352 + GFF4288 0.41 +1.7 72,355 - GFF4288 0.42 +1.7 72,398 + GFF4288 0.48 +1.5 72,421 + GFF4288 0.52 +1.2 72,582 + GFF4288 0.75 +1.8 72,707 - +1.1 72,780 - +0.4 72,780 - -0.2 72,854 + GFF4289 0.12 -0.2 72,855 - GFF4289 0.13 +0.4 72,921 + GFF4289 0.36 +0.5 72,922 - GFF4289 0.36 +0.8 72,924 - GFF4289 0.37 +0.6 72,924 - GFF4289 0.37 +0.7 73,014 - GFF4289 0.68 +0.0 73,083 + -0.9 73,084 - +0.3 73,257 + GFF4290 0.13 -0.5 73,374 + GFF4290 0.26 -3.0 73,605 + GFF4290 0.51 -1.4 73,605 + GFF4290 0.51 -2.9
Or see this region's nucleotide sequence