Experiment: LB_plus_SM_buffer with EIP16_phage 8.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4182 and GFF4183 overlap by 4 nucleotides GFF4183 and GFF4184 are separated by 129 nucleotides
GFF4182 - Ribosomal large subunit pseudouridine synthase D (EC 4.2.1.70), at 70,970 to 71,950
GFF4182
GFF4183 - COG1496: Uncharacterized conserved protein, at 71,947 to 72,678
GFF4183
GFF4184 - ClpB protein, at 72,808 to 75,381
GFF4184
Position (kb) 72
73
74
75
76 Strain fitness (log2 ratio) -1
0
1
2 at 71.828 kb on + strand, within GFF4182 at 71.881 kb on + strand at 71.882 kb on - strand at 71.894 kb on - strand at 71.926 kb on + strand at 72.220 kb on + strand, within GFF4183 at 72.228 kb on + strand, within GFF4183 at 72.405 kb on - strand, within GFF4183 at 72.768 kb on + strand at 72.768 kb on + strand at 72.778 kb on + strand at 72.826 kb on - strand at 72.828 kb on + strand at 72.828 kb on + strand at 72.944 kb on + strand at 72.944 kb on + strand at 72.953 kb on + strand at 72.954 kb on - strand at 73.669 kb on - strand, within GFF4184 at 73.771 kb on + strand, within GFF4184 at 73.771 kb on + strand, within GFF4184 at 73.934 kb on + strand, within GFF4184 at 73.935 kb on - strand, within GFF4184 at 73.935 kb on - strand, within GFF4184 at 74.229 kb on + strand, within GFF4184 at 74.232 kb on + strand, within GFF4184 at 74.391 kb on + strand, within GFF4184 at 74.504 kb on + strand, within GFF4184 at 74.558 kb on + strand, within GFF4184 at 74.653 kb on - strand, within GFF4184 at 74.671 kb on - strand, within GFF4184 at 74.765 kb on - strand, within GFF4184 at 74.961 kb on + strand, within GFF4184 at 74.961 kb on + strand, within GFF4184 at 75.076 kb on - strand, within GFF4184 at 75.104 kb on + strand, within GFF4184 at 75.105 kb on - strand, within GFF4184 at 75.126 kb on + strand at 75.155 kb on - strand at 75.155 kb on - strand at 75.155 kb on - strand at 75.189 kb on + strand at 75.364 kb on - strand at 75.522 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 8.75 MOI remove 71,828 + GFF4182 0.87 +2.4 71,881 + +2.5 71,882 - +2.6 71,894 - +2.2 71,926 + -0.4 72,220 + GFF4183 0.37 +0.1 72,228 + GFF4183 0.38 +0.5 72,405 - GFF4183 0.63 +0.5 72,768 + -0.5 72,768 + +0.6 72,778 + -0.3 72,826 - +0.3 72,828 + +0.5 72,828 + -0.6 72,944 + +0.5 72,944 + -0.1 72,953 + +1.1 72,954 - +0.4 73,669 - GFF4184 0.33 -1.1 73,771 + GFF4184 0.37 +1.1 73,771 + GFF4184 0.37 -0.2 73,934 + GFF4184 0.44 -0.1 73,935 - GFF4184 0.44 +0.6 73,935 - GFF4184 0.44 +0.2 74,229 + GFF4184 0.55 -0.3 74,232 + GFF4184 0.55 -0.2 74,391 + GFF4184 0.61 -0.2 74,504 + GFF4184 0.66 -0.2 74,558 + GFF4184 0.68 +0.1 74,653 - GFF4184 0.72 +0.7 74,671 - GFF4184 0.72 +0.6 74,765 - GFF4184 0.76 +0.4 74,961 + GFF4184 0.84 +0.5 74,961 + GFF4184 0.84 -0.0 75,076 - GFF4184 0.88 -0.4 75,104 + GFF4184 0.89 +0.1 75,105 - GFF4184 0.89 +0.5 75,126 + -0.5 75,155 - +0.3 75,155 - -0.4 75,155 - +0.2 75,189 + +0.2 75,364 - +0.2 75,522 - +0.2
Or see this region's nucleotide sequence