Experiment: LB_plus_SM_buffer with EIP16_phage 8.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF411 and GFF412 are separated by 298 nucleotides GFF412 and GFF413 are separated by 43 nucleotides
GFF411 - L-lactate permease, at 125,511 to 127,166
GFF411
GFF412 - Peptide transport system permease protein sapB (TC 3.A.1.5.5), at 127,465 to 131,850
GFF412
GFF413 - Putative lipoprotein, at 131,894 to 132,577
GFF413
Position (kb)
127
128
129
130
131
132 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 126.713 kb on + strand, within GFF411 at 126.956 kb on - strand, within GFF411 at 126.956 kb on - strand, within GFF411 at 127.048 kb on + strand at 127.048 kb on + strand at 127.049 kb on - strand at 127.049 kb on - strand at 127.049 kb on - strand at 127.049 kb on - strand at 127.068 kb on - strand at 127.233 kb on - strand at 127.286 kb on - strand at 127.317 kb on - strand at 127.346 kb on + strand at 127.458 kb on + strand at 127.466 kb on + strand at 127.533 kb on + strand at 127.558 kb on + strand at 127.788 kb on - strand at 127.804 kb on - strand at 127.877 kb on + strand at 127.877 kb on + strand at 127.878 kb on - strand at 128.028 kb on + strand, within GFF412 at 128.029 kb on - strand, within GFF412 at 128.031 kb on + strand, within GFF412 at 128.031 kb on + strand, within GFF412 at 128.060 kb on - strand, within GFF412 at 128.089 kb on - strand, within GFF412 at 128.163 kb on - strand, within GFF412 at 128.257 kb on - strand, within GFF412 at 128.325 kb on + strand, within GFF412 at 128.481 kb on + strand, within GFF412 at 128.482 kb on - strand, within GFF412 at 128.482 kb on - strand, within GFF412 at 128.509 kb on - strand, within GFF412 at 128.554 kb on - strand, within GFF412 at 128.554 kb on - strand, within GFF412 at 128.566 kb on - strand, within GFF412 at 128.663 kb on + strand, within GFF412 at 128.666 kb on + strand, within GFF412 at 128.666 kb on + strand, within GFF412 at 128.667 kb on - strand, within GFF412 at 128.667 kb on - strand, within GFF412 at 128.667 kb on - strand, within GFF412 at 128.680 kb on - strand, within GFF412 at 128.680 kb on - strand, within GFF412 at 128.680 kb on - strand, within GFF412 at 128.683 kb on - strand, within GFF412 at 128.708 kb on - strand, within GFF412 at 128.760 kb on + strand, within GFF412 at 128.771 kb on + strand, within GFF412 at 128.826 kb on + strand, within GFF412 at 128.826 kb on + strand, within GFF412 at 128.883 kb on + strand, within GFF412 at 128.895 kb on + strand, within GFF412 at 128.999 kb on - strand, within GFF412 at 129.026 kb on + strand, within GFF412 at 129.027 kb on - strand, within GFF412 at 129.051 kb on + strand, within GFF412 at 129.068 kb on + strand, within GFF412 at 129.096 kb on + strand, within GFF412 at 129.097 kb on - strand, within GFF412 at 129.097 kb on - strand, within GFF412 at 129.331 kb on - strand, within GFF412 at 129.412 kb on - strand, within GFF412 at 129.412 kb on - strand, within GFF412 at 129.453 kb on + strand, within GFF412 at 129.453 kb on + strand, within GFF412 at 129.540 kb on + strand, within GFF412 at 129.540 kb on + strand, within GFF412 at 129.544 kb on - strand, within GFF412 at 129.561 kb on + strand, within GFF412 at 129.738 kb on + strand, within GFF412 at 129.738 kb on + strand, within GFF412 at 129.817 kb on - strand, within GFF412 at 129.817 kb on - strand, within GFF412 at 129.823 kb on - strand, within GFF412 at 129.832 kb on - strand, within GFF412 at 130.104 kb on + strand, within GFF412 at 130.588 kb on - strand, within GFF412 at 130.870 kb on - strand, within GFF412 at 130.953 kb on + strand, within GFF412 at 130.954 kb on - strand, within GFF412 at 130.963 kb on + strand, within GFF412 at 130.964 kb on - strand, within GFF412 at 130.964 kb on - strand, within GFF412 at 130.971 kb on + strand, within GFF412 at 130.972 kb on - strand, within GFF412 at 130.975 kb on + strand, within GFF412 at 130.975 kb on + strand, within GFF412 at 130.976 kb on - strand, within GFF412 at 130.985 kb on + strand, within GFF412 at 130.985 kb on + strand, within GFF412 at 131.000 kb on - strand, within GFF412 at 131.007 kb on - strand, within GFF412 at 131.026 kb on - strand, within GFF412 at 131.026 kb on - strand, within GFF412 at 131.080 kb on + strand, within GFF412 at 131.116 kb on - strand, within GFF412 at 131.147 kb on + strand, within GFF412 at 131.147 kb on + strand, within GFF412 at 131.147 kb on + strand, within GFF412 at 131.147 kb on + strand, within GFF412 at 131.147 kb on + strand, within GFF412 at 131.148 kb on - strand, within GFF412 at 131.148 kb on - strand, within GFF412 at 131.148 kb on - strand, within GFF412 at 131.148 kb on - strand, within GFF412 at 131.148 kb on - strand, within GFF412 at 131.148 kb on - strand, within GFF412 at 131.148 kb on - strand, within GFF412 at 131.148 kb on - strand, within GFF412 at 131.300 kb on + strand, within GFF412 at 131.300 kb on + strand, within GFF412 at 131.305 kb on + strand, within GFF412 at 131.305 kb on + strand, within GFF412 at 131.306 kb on - strand, within GFF412 at 131.306 kb on - strand, within GFF412 at 131.340 kb on + strand, within GFF412 at 131.340 kb on + strand, within GFF412 at 131.341 kb on - strand, within GFF412 at 131.347 kb on + strand, within GFF412 at 131.382 kb on - strand, within GFF412 at 131.403 kb on - strand, within GFF412 at 131.550 kb on + strand at 131.551 kb on - strand at 131.593 kb on - strand at 131.836 kb on - strand at 132.224 kb on - strand, within GFF413 at 132.301 kb on + strand, within GFF413 at 132.301 kb on + strand, within GFF413
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 8.75 MOI remove 126,713 + GFF411 0.73 -0.2 126,956 - GFF411 0.87 -0.2 126,956 - GFF411 0.87 -0.3 127,048 + -0.3 127,048 + +0.8 127,049 - +0.7 127,049 - +0.1 127,049 - +0.6 127,049 - -0.0 127,068 - +1.4 127,233 - +0.5 127,286 - +0.0 127,317 - +0.3 127,346 + +1.6 127,458 + -0.0 127,466 + +0.1 127,533 + -0.9 127,558 + -0.1 127,788 - +0.0 127,804 - -0.1 127,877 + +0.7 127,877 + -0.7 127,878 - +0.8 128,028 + GFF412 0.13 +0.4 128,029 - GFF412 0.13 +0.5 128,031 + GFF412 0.13 +0.4 128,031 + GFF412 0.13 -0.0 128,060 - GFF412 0.14 -0.3 128,089 - GFF412 0.14 +0.1 128,163 - GFF412 0.16 +0.1 128,257 - GFF412 0.18 +2.7 128,325 + GFF412 0.20 -2.1 128,481 + GFF412 0.23 +0.3 128,482 - GFF412 0.23 +1.6 128,482 - GFF412 0.23 +2.6 128,509 - GFF412 0.24 +1.0 128,554 - GFF412 0.25 +0.2 128,554 - GFF412 0.25 -0.7 128,566 - GFF412 0.25 +0.1 128,663 + GFF412 0.27 -0.4 128,666 + GFF412 0.27 -1.8 128,666 + GFF412 0.27 +0.4 128,667 - GFF412 0.27 +0.1 128,667 - GFF412 0.27 +0.6 128,667 - GFF412 0.27 -0.3 128,680 - GFF412 0.28 -1.7 128,680 - GFF412 0.28 -1.1 128,680 - GFF412 0.28 -0.0 128,683 - GFF412 0.28 +0.4 128,708 - GFF412 0.28 -0.2 128,760 + GFF412 0.30 -0.2 128,771 + GFF412 0.30 -0.2 128,826 + GFF412 0.31 +0.5 128,826 + GFF412 0.31 -0.5 128,883 + GFF412 0.32 -0.1 128,895 + GFF412 0.33 -0.0 128,999 - GFF412 0.35 -0.9 129,026 + GFF412 0.36 +0.5 129,027 - GFF412 0.36 +0.1 129,051 + GFF412 0.36 -0.0 129,068 + GFF412 0.37 -0.2 129,096 + GFF412 0.37 -0.2 129,097 - GFF412 0.37 -0.1 129,097 - GFF412 0.37 +0.1 129,331 - GFF412 0.43 -0.6 129,412 - GFF412 0.44 -1.3 129,412 - GFF412 0.44 +0.0 129,453 + GFF412 0.45 +0.2 129,453 + GFF412 0.45 +0.1 129,540 + GFF412 0.47 -0.3 129,540 + GFF412 0.47 -0.9 129,544 - GFF412 0.47 -1.1 129,561 + GFF412 0.48 -1.1 129,738 + GFF412 0.52 +1.4 129,738 + GFF412 0.52 +0.2 129,817 - GFF412 0.54 +0.1 129,817 - GFF412 0.54 -1.5 129,823 - GFF412 0.54 +1.1 129,832 - GFF412 0.54 -0.3 130,104 + GFF412 0.60 -0.0 130,588 - GFF412 0.71 -0.5 130,870 - GFF412 0.78 -1.4 130,953 + GFF412 0.80 -0.3 130,954 - GFF412 0.80 -0.1 130,963 + GFF412 0.80 +0.1 130,964 - GFF412 0.80 +0.2 130,964 - GFF412 0.80 -1.0 130,971 + GFF412 0.80 +2.3 130,972 - GFF412 0.80 -1.0 130,975 + GFF412 0.80 +0.2 130,975 + GFF412 0.80 +1.0 130,976 - GFF412 0.80 -1.4 130,985 + GFF412 0.80 -0.5 130,985 + GFF412 0.80 +1.5 131,000 - GFF412 0.81 +1.0 131,007 - GFF412 0.81 +0.1 131,026 - GFF412 0.81 -1.2 131,026 - GFF412 0.81 -0.2 131,080 + GFF412 0.82 -2.0 131,116 - GFF412 0.83 +0.0 131,147 + GFF412 0.84 -0.2 131,147 + GFF412 0.84 -0.2 131,147 + GFF412 0.84 +2.6 131,147 + GFF412 0.84 -0.7 131,147 + GFF412 0.84 +0.0 131,148 - GFF412 0.84 +0.1 131,148 - GFF412 0.84 -0.1 131,148 - GFF412 0.84 +0.6 131,148 - GFF412 0.84 -0.8 131,148 - GFF412 0.84 -1.4 131,148 - GFF412 0.84 +0.1 131,148 - GFF412 0.84 +0.1 131,148 - GFF412 0.84 -0.1 131,300 + GFF412 0.87 -0.1 131,300 + GFF412 0.87 -0.6 131,305 + GFF412 0.88 +0.1 131,305 + GFF412 0.88 +0.4 131,306 - GFF412 0.88 -0.5 131,306 - GFF412 0.88 -0.1 131,340 + GFF412 0.88 -0.6 131,340 + GFF412 0.88 -0.6 131,341 - GFF412 0.88 +0.4 131,347 + GFF412 0.89 -0.8 131,382 - GFF412 0.89 -0.3 131,403 - GFF412 0.90 +0.4 131,550 + +0.7 131,551 - +0.0 131,593 - +0.1 131,836 - +1.5 132,224 - GFF413 0.48 -0.7 132,301 + GFF413 0.60 +0.1 132,301 + GFF413 0.60 +0.5
Or see this region's nucleotide sequence