Experiment: LB_plus_SM_buffer with EIP16_phage 8.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3966 and GFF3967 overlap by 4 nucleotides GFF3967 and GFF3968 overlap by 4 nucleotides GFF3968 and GFF3969 are separated by 26 nucleotides
GFF3966 - Cobyric acid synthase (EC 6.3.5.10), at 243,651 to 245,171
GFF3966
GFF3967 - Adenosylcobinamide-phosphate guanylyltransferase (EC 2.7.7.62), at 245,168 to 245,713
GFF3967
GFF3968 - Cobalamin synthase (EC 2.7.8.26), at 245,710 to 246,453
GFF3968
GFF3969 - Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (EC 2.4.2.21), at 246,480 to 247,550
GFF3969
Position (kb) 245
246 Strain fitness (log2 ratio) -1
0
1
2 at 244.219 kb on + strand, within GFF3966 at 244.233 kb on - strand, within GFF3966 at 244.266 kb on + strand, within GFF3966 at 244.267 kb on - strand, within GFF3966 at 244.434 kb on - strand, within GFF3966 at 244.434 kb on - strand, within GFF3966 at 244.434 kb on - strand, within GFF3966 at 244.434 kb on - strand, within GFF3966 at 244.454 kb on + strand, within GFF3966 at 244.491 kb on + strand, within GFF3966 at 244.728 kb on - strand, within GFF3966 at 245.253 kb on - strand, within GFF3967 at 245.253 kb on - strand, within GFF3967 at 245.254 kb on + strand, within GFF3967 at 245.309 kb on - strand, within GFF3967 at 245.413 kb on + strand, within GFF3967 at 245.413 kb on + strand, within GFF3967 at 245.414 kb on - strand, within GFF3967 at 245.559 kb on + strand, within GFF3967 at 245.849 kb on + strand, within GFF3968 at 246.182 kb on - strand, within GFF3968 at 246.182 kb on - strand, within GFF3968 at 246.222 kb on - strand, within GFF3968 at 246.259 kb on - strand, within GFF3968 at 246.303 kb on + strand, within GFF3968 at 246.366 kb on + strand, within GFF3968 at 246.450 kb on - strand at 246.479 kb on + strand at 246.479 kb on + strand at 246.480 kb on - strand at 246.490 kb on + strand at 246.554 kb on + strand at 246.635 kb on + strand, within GFF3969
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 8.75 MOI remove 244,219 + GFF3966 0.37 -0.3 244,233 - GFF3966 0.38 +0.1 244,266 + GFF3966 0.40 -0.1 244,267 - GFF3966 0.40 -0.1 244,434 - GFF3966 0.51 -0.8 244,434 - GFF3966 0.51 +1.6 244,434 - GFF3966 0.51 +0.1 244,434 - GFF3966 0.51 +1.0 244,454 + GFF3966 0.53 -0.1 244,491 + GFF3966 0.55 -0.1 244,728 - GFF3966 0.71 -0.9 245,253 - GFF3967 0.16 -0.8 245,253 - GFF3967 0.16 -0.4 245,254 + GFF3967 0.16 +0.1 245,309 - GFF3967 0.26 -0.3 245,413 + GFF3967 0.45 -0.1 245,413 + GFF3967 0.45 -0.0 245,414 - GFF3967 0.45 -0.7 245,559 + GFF3967 0.72 -0.7 245,849 + GFF3968 0.19 -0.8 246,182 - GFF3968 0.63 -0.5 246,182 - GFF3968 0.63 -0.9 246,222 - GFF3968 0.69 -0.2 246,259 - GFF3968 0.74 +2.5 246,303 + GFF3968 0.80 +1.1 246,366 + GFF3968 0.88 +2.5 246,450 - -0.9 246,479 + +0.6 246,479 + -1.0 246,480 - -0.0 246,490 + -0.5 246,554 + +0.3 246,635 + GFF3969 0.14 -0.4
Or see this region's nucleotide sequence