Experiment: LB_plus_SM_buffer with EIP16_phage 8.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3798 and GFF3799 are separated by 125 nucleotides GFF3799 and GFF3800 are separated by 11 nucleotides
GFF3798 - Putative transcriptional regulator, at 67,062 to 67,970
GFF3798
GFF3799 - 4-hydroxybenzoate transporter, at 68,096 to 69,454
GFF3799
GFF3800 - Gentisate 1,2-dioxygenase (EC 1.13.11.4), at 69,466 to 70,503
GFF3800
Position (kb)
68
69
70 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 67.215 kb on + strand, within GFF3798 at 67.227 kb on - strand, within GFF3798 at 67.235 kb on + strand, within GFF3798 at 67.335 kb on - strand, within GFF3798 at 67.335 kb on - strand, within GFF3798 at 67.581 kb on - strand, within GFF3798 at 67.733 kb on + strand, within GFF3798 at 67.766 kb on + strand, within GFF3798 at 67.786 kb on - strand, within GFF3798 at 67.786 kb on - strand, within GFF3798 at 67.871 kb on + strand, within GFF3798 at 67.871 kb on + strand, within GFF3798 at 67.872 kb on - strand, within GFF3798 at 67.941 kb on + strand at 68.048 kb on + strand at 68.049 kb on - strand at 68.257 kb on - strand, within GFF3799 at 68.424 kb on + strand, within GFF3799 at 68.561 kb on - strand, within GFF3799 at 68.561 kb on - strand, within GFF3799 at 68.950 kb on + strand, within GFF3799 at 69.072 kb on - strand, within GFF3799 at 69.072 kb on - strand, within GFF3799 at 69.102 kb on + strand, within GFF3799 at 69.164 kb on - strand, within GFF3799 at 69.326 kb on - strand at 69.488 kb on + strand at 69.520 kb on - strand at 69.632 kb on + strand, within GFF3800 at 69.740 kb on - strand, within GFF3800 at 69.749 kb on + strand, within GFF3800 at 69.813 kb on + strand, within GFF3800 at 70.045 kb on + strand, within GFF3800 at 70.046 kb on - strand, within GFF3800 at 70.123 kb on + strand, within GFF3800 at 70.308 kb on + strand, within GFF3800 at 70.309 kb on - strand, within GFF3800 at 70.309 kb on - strand, within GFF3800 at 70.309 kb on - strand, within GFF3800 at 70.309 kb on - strand, within GFF3800 at 70.312 kb on + strand, within GFF3800 at 70.312 kb on + strand, within GFF3800
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 8.75 MOI remove 67,215 + GFF3798 0.17 +1.0 67,227 - GFF3798 0.18 +1.3 67,235 + GFF3798 0.19 +2.2 67,335 - GFF3798 0.30 +0.1 67,335 - GFF3798 0.30 -0.4 67,581 - GFF3798 0.57 -0.3 67,733 + GFF3798 0.74 +0.2 67,766 + GFF3798 0.77 +0.1 67,786 - GFF3798 0.80 -0.1 67,786 - GFF3798 0.80 -1.3 67,871 + GFF3798 0.89 +3.4 67,871 + GFF3798 0.89 -0.4 67,872 - GFF3798 0.89 -0.5 67,941 + +1.2 68,048 + -0.5 68,049 - +0.3 68,257 - GFF3799 0.12 -0.9 68,424 + GFF3799 0.24 +0.7 68,561 - GFF3799 0.34 -0.7 68,561 - GFF3799 0.34 -1.0 68,950 + GFF3799 0.63 -0.1 69,072 - GFF3799 0.72 +0.1 69,072 - GFF3799 0.72 -0.4 69,102 + GFF3799 0.74 +0.0 69,164 - GFF3799 0.79 +0.3 69,326 - -0.4 69,488 + -1.4 69,520 - -0.0 69,632 + GFF3800 0.16 +0.5 69,740 - GFF3800 0.26 -0.5 69,749 + GFF3800 0.27 -0.4 69,813 + GFF3800 0.33 -0.9 70,045 + GFF3800 0.56 -0.1 70,046 - GFF3800 0.56 -0.0 70,123 + GFF3800 0.63 -1.6 70,308 + GFF3800 0.81 +0.5 70,309 - GFF3800 0.81 -0.4 70,309 - GFF3800 0.81 -0.1 70,309 - GFF3800 0.81 +0.0 70,309 - GFF3800 0.81 -0.3 70,312 + GFF3800 0.82 +0.3 70,312 + GFF3800 0.82 -2.0
Or see this region's nucleotide sequence