Experiment: LB_plus_SM_buffer with EIP16_phage 8.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3620 and GFF3621 are separated by 334 nucleotides GFF3621 and GFF3622 are separated by 77 nucleotides GFF3622 and GFF3623 are separated by 100 nucleotides GFF3623 and GFF3624 are separated by 68 nucleotides
GFF3620 - FIG00545237: hypothetical protein, at 372,174 to 372,995
GFF3620
GFF3621 - Glutaredoxin-related protein, at 373,330 to 373,677
GFF3621
GFF3622 - Ribonuclease T (EC 3.1.13.-), at 373,755 to 374,402
GFF3622
GFF3623 - Lactoylglutathione lyase (EC 4.4.1.5), at 374,503 to 374,910
GFF3623
GFF3624 - N-ethylmaleimide reductase (EC 1.-.-.-), at 374,979 to 376,076
GFF3624
Position (kb) 373
374
375 Strain fitness (log2 ratio) -1
0
1 at 373.238 kb on + strand at 373.388 kb on - strand, within GFF3621 at 373.389 kb on + strand, within GFF3621 at 373.474 kb on + strand, within GFF3621 at 373.601 kb on - strand, within GFF3621 at 373.627 kb on - strand, within GFF3621 at 373.649 kb on - strand at 373.650 kb on + strand at 373.756 kb on + strand at 373.777 kb on - strand at 373.777 kb on - strand at 374.414 kb on - strand at 374.715 kb on - strand, within GFF3623 at 374.715 kb on - strand, within GFF3623 at 374.892 kb on + strand at 374.893 kb on - strand at 374.896 kb on - strand at 375.097 kb on + strand, within GFF3624 at 375.098 kb on - strand, within GFF3624 at 375.166 kb on + strand, within GFF3624 at 375.266 kb on + strand, within GFF3624 at 375.267 kb on - strand, within GFF3624 at 375.267 kb on - strand, within GFF3624 at 375.275 kb on - strand, within GFF3624
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 8.75 MOI remove 373,238 + -0.0 373,388 - GFF3621 0.17 -0.5 373,389 + GFF3621 0.17 -1.5 373,474 + GFF3621 0.41 -0.9 373,601 - GFF3621 0.78 -0.8 373,627 - GFF3621 0.85 -1.1 373,649 - -0.4 373,650 + -0.1 373,756 + +0.7 373,777 - +0.6 373,777 - +0.2 374,414 - +0.7 374,715 - GFF3623 0.52 -0.8 374,715 - GFF3623 0.52 +0.6 374,892 + +0.4 374,893 - -1.3 374,896 - -0.0 375,097 + GFF3624 0.11 -0.9 375,098 - GFF3624 0.11 -0.4 375,166 + GFF3624 0.17 -0.5 375,266 + GFF3624 0.26 -0.2 375,267 - GFF3624 0.26 +0.8 375,267 - GFF3624 0.26 -0.1 375,275 - GFF3624 0.27 -0.4
Or see this region's nucleotide sequence