Experiment: LB_plus_SM_buffer with EIP16_phage 8.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3514 and GFF3515 are separated by 106 nucleotides GFF3515 and GFF3516 are separated by 96 nucleotides GFF3516 and GFF3517 are separated by 321 nucleotides
GFF3514 - Ribulosamine/erythrulosamine 3-kinase potentially involved in protein deglycation, at 265,789 to 266,649
GFF3514
GFF3515 - FIG00637864: hypothetical protein, at 266,756 to 267,046
GFF3515
GFF3516 - 6-phosphofructokinase class II (EC 2.7.1.11), at 267,143 to 268,075
GFF3516
GFF3517 - Putative outer membrane protein, at 268,397 to 269,122
GFF3517
Position (kb) 267
268
269 Strain fitness (log2 ratio) -1
0
1 at 266.157 kb on + strand, within GFF3514 at 266.157 kb on + strand, within GFF3514 at 266.158 kb on - strand, within GFF3514 at 266.546 kb on - strand, within GFF3514 at 266.649 kb on + strand at 266.712 kb on - strand at 266.718 kb on + strand at 266.812 kb on + strand, within GFF3515 at 266.812 kb on + strand, within GFF3515 at 267.077 kb on + strand at 267.166 kb on - strand at 267.202 kb on + strand at 267.305 kb on - strand, within GFF3516 at 267.378 kb on - strand, within GFF3516 at 267.440 kb on + strand, within GFF3516 at 267.577 kb on + strand, within GFF3516 at 267.670 kb on + strand, within GFF3516 at 267.949 kb on + strand, within GFF3516 at 267.949 kb on + strand, within GFF3516 at 268.061 kb on - strand at 268.063 kb on - strand at 268.066 kb on + strand at 268.067 kb on - strand at 268.067 kb on - strand at 268.358 kb on - strand at 268.359 kb on + strand at 268.427 kb on + strand at 268.589 kb on - strand, within GFF3517 at 268.702 kb on + strand, within GFF3517 at 268.852 kb on + strand, within GFF3517 at 268.853 kb on - strand, within GFF3517 at 268.902 kb on - strand, within GFF3517 at 268.927 kb on + strand, within GFF3517 at 268.928 kb on - strand, within GFF3517 at 268.974 kb on + strand, within GFF3517 at 268.975 kb on - strand, within GFF3517 at 269.040 kb on + strand, within GFF3517
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 8.75 MOI remove 266,157 + GFF3514 0.43 +1.4 266,157 + GFF3514 0.43 +0.2 266,158 - GFF3514 0.43 -0.0 266,546 - GFF3514 0.88 +0.1 266,649 + -0.0 266,712 - -0.5 266,718 + +0.5 266,812 + GFF3515 0.19 +0.8 266,812 + GFF3515 0.19 +0.3 267,077 + +1.3 267,166 - -1.0 267,202 + -0.4 267,305 - GFF3516 0.17 -0.3 267,378 - GFF3516 0.25 -0.3 267,440 + GFF3516 0.32 -0.1 267,577 + GFF3516 0.47 -0.1 267,670 + GFF3516 0.56 +0.5 267,949 + GFF3516 0.86 +1.3 267,949 + GFF3516 0.86 -0.0 268,061 - -0.7 268,063 - +0.5 268,066 + +0.1 268,067 - -0.4 268,067 - +0.3 268,358 - +0.3 268,359 + +0.4 268,427 + -0.0 268,589 - GFF3517 0.26 -1.2 268,702 + GFF3517 0.42 -0.4 268,852 + GFF3517 0.63 -0.0 268,853 - GFF3517 0.63 -0.3 268,902 - GFF3517 0.70 +0.3 268,927 + GFF3517 0.73 +1.0 268,928 - GFF3517 0.73 -0.3 268,974 + GFF3517 0.79 +0.8 268,975 - GFF3517 0.80 -0.0 269,040 + GFF3517 0.89 +0.2
Or see this region's nucleotide sequence