Experiment: LB_plus_SM_buffer with EIP16_phage 8.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3418 and GFF3419 are separated by 53 nucleotides GFF3419 and GFF3420 are separated by 11 nucleotides GFF3420 and GFF3421 overlap by 4 nucleotides GFF3421 and GFF3422 are separated by 99 nucleotides
GFF3418 - tRNA-specific 2-thiouridylase MnmA, at 183,641 to 184,747
GFF3418
GFF3419 - Nudix-like NDP and NTP phosphohydrolase YmfB, at 184,801 to 185,262
GFF3419
GFF3420 - FIG01056067: hypothetical protein, at 185,274 to 185,603
GFF3420
GFF3421 - Ribosomal large subunit pseudouridine synthase E (EC 4.2.1.70), at 185,600 to 186,337
GFF3421
GFF3422 - Isocitrate dehydrogenase [NADP] (EC 1.1.1.42), at 186,437 to 187,687
GFF3422
Position (kb) 185
186 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 184.845 kb on + strand at 184.874 kb on + strand, within GFF3419 at 185.215 kb on - strand, within GFF3419 at 185.227 kb on + strand at 185.227 kb on + strand at 185.262 kb on + strand at 185.285 kb on + strand at 185.677 kb on - strand, within GFF3421 at 185.749 kb on - strand, within GFF3421 at 185.749 kb on - strand, within GFF3421 at 185.749 kb on - strand, within GFF3421 at 185.749 kb on - strand, within GFF3421 at 185.749 kb on - strand, within GFF3421 at 185.749 kb on - strand, within GFF3421 at 185.750 kb on + strand, within GFF3421 at 185.750 kb on + strand, within GFF3421 at 185.750 kb on + strand, within GFF3421 at 185.750 kb on + strand, within GFF3421 at 185.751 kb on - strand, within GFF3421 at 185.779 kb on + strand, within GFF3421 at 185.779 kb on + strand, within GFF3421 at 185.887 kb on + strand, within GFF3421 at 185.888 kb on - strand, within GFF3421 at 185.928 kb on - strand, within GFF3421 at 185.937 kb on + strand, within GFF3421 at 185.937 kb on + strand, within GFF3421 at 185.937 kb on + strand, within GFF3421 at 185.938 kb on - strand, within GFF3421 at 185.938 kb on - strand, within GFF3421 at 186.068 kb on - strand, within GFF3421 at 186.268 kb on + strand at 186.314 kb on - strand at 186.328 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 8.75 MOI remove 184,845 + +0.4 184,874 + GFF3419 0.16 +0.5 185,215 - GFF3419 0.90 +0.5 185,227 + +0.7 185,227 + -1.4 185,262 + -0.6 185,285 + +0.1 185,677 - GFF3421 0.10 +0.5 185,749 - GFF3421 0.20 +0.3 185,749 - GFF3421 0.20 +2.2 185,749 - GFF3421 0.20 -0.9 185,749 - GFF3421 0.20 -0.3 185,749 - GFF3421 0.20 -1.0 185,749 - GFF3421 0.20 +0.1 185,750 + GFF3421 0.20 +0.7 185,750 + GFF3421 0.20 +0.5 185,750 + GFF3421 0.20 +0.8 185,750 + GFF3421 0.20 +3.0 185,751 - GFF3421 0.20 -0.1 185,779 + GFF3421 0.24 -2.9 185,779 + GFF3421 0.24 +2.2 185,887 + GFF3421 0.39 -0.3 185,888 - GFF3421 0.39 -0.1 185,928 - GFF3421 0.44 -0.3 185,937 + GFF3421 0.46 -0.1 185,937 + GFF3421 0.46 -0.5 185,937 + GFF3421 0.46 +0.2 185,938 - GFF3421 0.46 +1.5 185,938 - GFF3421 0.46 +0.4 186,068 - GFF3421 0.63 -0.7 186,268 + +0.2 186,314 - -1.6 186,328 + -0.0
Or see this region's nucleotide sequence