Experiment: LB_plus_SM_buffer with EIP16_phage 8.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3402 and GFF3403 are separated by 18 nucleotides GFF3403 and GFF3404 are separated by 81 nucleotides GFF3404 and GFF3405 are separated by 24 nucleotides GFF3405 and GFF3406 are separated by 102 nucleotides
GFF3402 - N-acetyl-D-glucosamine kinase (EC 2.7.1.59), at 167,897 to 168,808
GFF3402
GFF3403 - NAD-dependent protein deacetylase of SIR2 family, at 168,827 to 169,648
GFF3403
GFF3404 - ABC transporter, periplasmic spermidine putrescine-binding protein PotD (TC 3.A.1.11.1), at 169,730 to 170,776
GFF3404
GFF3405 - Spermidine Putrescine ABC transporter permease component potC (TC_3.A.1.11.1), at 170,801 to 171,580
GFF3405
GFF3406 - FIG01047625: hypothetical protein, at 171,683 to 171,805
GFF3406
Position (kb) 169
170
171 Strain fitness (log2 ratio) -2
-1
0
1 at 168.954 kb on + strand, within GFF3403 at 169.102 kb on + strand, within GFF3403 at 169.102 kb on + strand, within GFF3403 at 169.456 kb on + strand, within GFF3403 at 169.531 kb on + strand, within GFF3403 at 169.627 kb on + strand at 169.646 kb on + strand at 169.646 kb on + strand at 169.731 kb on + strand at 169.758 kb on + strand at 169.758 kb on + strand at 170.300 kb on - strand, within GFF3404 at 170.351 kb on - strand, within GFF3404 at 170.390 kb on - strand, within GFF3404 at 170.391 kb on + strand, within GFF3404 at 170.521 kb on - strand, within GFF3404 at 170.521 kb on - strand, within GFF3404 at 170.521 kb on - strand, within GFF3404 at 170.523 kb on + strand, within GFF3404 at 170.524 kb on - strand, within GFF3404 at 170.548 kb on - strand, within GFF3404 at 170.581 kb on - strand, within GFF3404 at 170.581 kb on - strand, within GFF3404 at 170.601 kb on + strand, within GFF3404 at 170.601 kb on + strand, within GFF3404 at 170.602 kb on - strand, within GFF3404 at 170.668 kb on - strand, within GFF3404 at 170.668 kb on - strand, within GFF3404 at 170.680 kb on + strand at 170.689 kb on - strand at 170.779 kb on - strand at 170.830 kb on + strand at 171.223 kb on + strand, within GFF3405 at 171.272 kb on + strand, within GFF3405 at 171.276 kb on - strand, within GFF3405 at 171.276 kb on - strand, within GFF3405 at 171.524 kb on - strand at 171.524 kb on - strand at 171.531 kb on + strand at 171.537 kb on + strand at 171.598 kb on - strand at 171.767 kb on + strand, within GFF3406 at 171.768 kb on - strand, within GFF3406
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 8.75 MOI remove 168,954 + GFF3403 0.15 +0.2 169,102 + GFF3403 0.33 -0.2 169,102 + GFF3403 0.33 -0.0 169,456 + GFF3403 0.77 -0.5 169,531 + GFF3403 0.86 -0.8 169,627 + -0.7 169,646 + -2.0 169,646 + -0.7 169,731 + -0.0 169,758 + -0.2 169,758 + +0.7 170,300 - GFF3404 0.54 -1.3 170,351 - GFF3404 0.59 -0.4 170,390 - GFF3404 0.63 -0.4 170,391 + GFF3404 0.63 -1.2 170,521 - GFF3404 0.76 -0.8 170,521 - GFF3404 0.76 -0.8 170,521 - GFF3404 0.76 -1.9 170,523 + GFF3404 0.76 -1.4 170,524 - GFF3404 0.76 +0.7 170,548 - GFF3404 0.78 -1.0 170,581 - GFF3404 0.81 -0.3 170,581 - GFF3404 0.81 -0.9 170,601 + GFF3404 0.83 -1.3 170,601 + GFF3404 0.83 -1.0 170,602 - GFF3404 0.83 -0.5 170,668 - GFF3404 0.90 -0.3 170,668 - GFF3404 0.90 -1.0 170,680 + -0.3 170,689 - -0.2 170,779 - -0.8 170,830 + -1.4 171,223 + GFF3405 0.54 +1.0 171,272 + GFF3405 0.60 -0.8 171,276 - GFF3405 0.61 -0.2 171,276 - GFF3405 0.61 -0.3 171,524 - -0.9 171,524 - -1.1 171,531 + -1.4 171,537 + -0.4 171,598 - -0.0 171,767 + GFF3406 0.68 +0.1 171,768 - GFF3406 0.69 +0.2
Or see this region's nucleotide sequence