Experiment: LB_plus_SM_buffer with EIP16_phage 8.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3315 and GFF3316 are separated by 88 nucleotides GFF3316 and GFF3317 are separated by 38 nucleotides GFF3317 and GFF3318 are separated by 26 nucleotides GFF3318 and GFF3319 are separated by 26 nucleotides GFF3319 and GFF3320 are separated by 4 nucleotides
GFF3315 - Putative oxidoreductase component of anaerobic dehydrogenases; Functional role page for Chaperone protein TorD, at 88,900 to 89,454
GFF3315
GFF3316 - Inner membrane protein YcdZ, at 89,543 to 90,037
GFF3316
GFF3317 - Curli production assembly/transport component CsgG, at 90,076 to 90,909
GFF3317
GFF3318 - Curli production assembly/transport component CsgF, at 90,936 to 91,352
GFF3318
GFF3319 - Curli production assembly/transport component CsgE, at 91,379 to 91,774
GFF3319
GFF3320 - Transcriptional regulator CsgD for 2nd curli operon, at 91,779 to 92,429
GFF3320
Position (kb) 90
91 Strain fitness (log2 ratio) -1
0
1
2 at 89.334 kb on + strand, within GFF3315 at 89.452 kb on + strand at 89.463 kb on - strand at 89.573 kb on - strand at 89.573 kb on - strand at 89.637 kb on + strand, within GFF3316 at 89.719 kb on + strand, within GFF3316 at 89.735 kb on - strand, within GFF3316 at 89.929 kb on - strand, within GFF3316 at 90.070 kb on + strand at 90.070 kb on + strand at 90.070 kb on + strand at 90.121 kb on - strand at 90.249 kb on - strand, within GFF3317 at 90.307 kb on + strand, within GFF3317 at 90.308 kb on - strand, within GFF3317 at 90.430 kb on - strand, within GFF3317 at 90.487 kb on - strand, within GFF3317 at 90.703 kb on - strand, within GFF3317 at 90.747 kb on + strand, within GFF3317 at 90.750 kb on - strand, within GFF3317 at 90.784 kb on + strand, within GFF3317 at 90.915 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 8.75 MOI remove 89,334 + GFF3315 0.78 -0.7 89,452 + +0.4 89,463 - -0.5 89,573 - -0.0 89,573 - -0.4 89,637 + GFF3316 0.19 +0.4 89,719 + GFF3316 0.36 -0.8 89,735 - GFF3316 0.39 +0.4 89,929 - GFF3316 0.78 +0.6 90,070 + +1.3 90,070 + +0.3 90,070 + -1.0 90,121 - -0.6 90,249 - GFF3317 0.21 -0.3 90,307 + GFF3317 0.28 -0.1 90,308 - GFF3317 0.28 +2.5 90,430 - GFF3317 0.42 +1.5 90,487 - GFF3317 0.49 +0.4 90,703 - GFF3317 0.75 -0.4 90,747 + GFF3317 0.80 -0.3 90,750 - GFF3317 0.81 -0.4 90,784 + GFF3317 0.85 +1.2 90,915 - -0.4
Or see this region's nucleotide sequence