Experiment: LB_plus_SM_buffer with EIP16_phage 8.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3299 and GFF3300 overlap by 4 nucleotides GFF3300 and GFF3301 are separated by 58 nucleotides GFF3301 and GFF3302 are separated by 60 nucleotides GFF3302 and GFF3303 are separated by 229 nucleotides
GFF3299 - Transcriptional regulator RutR of pyrimidine catabolism (TetR family), at 68,374 to 69,012
GFF3299
GFF3300 - Putative secreted protein, at 69,009 to 69,404
GFF3300
GFF3301 - Transcriptional repressor of PutA and PutP / Proline dehydrogenase (EC 1.5.99.8) (Proline oxidase) / Delta-1-pyrroline-5-carboxylate dehydrogenase (EC 1.2.1.88), at 69,463 to 73,425
GFF3301
GFF3302 - FIG00639374: hypothetical protein, at 73,486 to 73,617
GFF3302
GFF3303 - Proline/sodium symporter PutP (TC 2.A.21.2.1) @ Propionate/sodium symporter, at 73,847 to 75,355
GFF3303
Position (kb) 69
70
71
72
73
74 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 68.548 kb on + strand, within GFF3299 at 68.569 kb on + strand, within GFF3299 at 68.664 kb on + strand, within GFF3299 at 68.775 kb on - strand, within GFF3299 at 68.787 kb on - strand, within GFF3299 at 68.890 kb on + strand, within GFF3299 at 68.985 kb on + strand at 68.985 kb on + strand at 69.404 kb on + strand at 69.404 kb on + strand at 69.448 kb on + strand at 69.449 kb on - strand at 69.551 kb on - strand at 69.553 kb on - strand at 69.553 kb on - strand at 69.845 kb on - strand at 69.845 kb on - strand at 70.009 kb on + strand, within GFF3301 at 70.010 kb on - strand, within GFF3301 at 70.021 kb on - strand, within GFF3301 at 70.198 kb on - strand, within GFF3301 at 70.209 kb on - strand, within GFF3301 at 70.261 kb on - strand, within GFF3301 at 70.266 kb on + strand, within GFF3301 at 70.267 kb on - strand, within GFF3301 at 70.270 kb on + strand, within GFF3301 at 70.270 kb on + strand, within GFF3301 at 70.270 kb on + strand, within GFF3301 at 70.271 kb on - strand, within GFF3301 at 70.271 kb on - strand, within GFF3301 at 70.338 kb on + strand, within GFF3301 at 70.341 kb on - strand, within GFF3301 at 70.341 kb on - strand, within GFF3301 at 70.616 kb on - strand, within GFF3301 at 70.616 kb on - strand, within GFF3301 at 70.759 kb on - strand, within GFF3301 at 71.412 kb on + strand, within GFF3301 at 71.718 kb on + strand, within GFF3301 at 71.764 kb on + strand, within GFF3301 at 71.772 kb on - strand, within GFF3301 at 71.864 kb on + strand, within GFF3301 at 71.865 kb on - strand, within GFF3301 at 71.904 kb on - strand, within GFF3301 at 71.939 kb on + strand, within GFF3301 at 72.029 kb on + strand, within GFF3301 at 72.179 kb on + strand, within GFF3301 at 72.180 kb on - strand, within GFF3301 at 72.441 kb on + strand, within GFF3301 at 72.465 kb on - strand, within GFF3301 at 72.576 kb on - strand, within GFF3301 at 72.809 kb on - strand, within GFF3301 at 72.809 kb on - strand, within GFF3301 at 72.874 kb on + strand, within GFF3301 at 72.889 kb on - strand, within GFF3301 at 73.268 kb on - strand at 73.287 kb on + strand at 73.307 kb on + strand at 73.308 kb on - strand at 73.311 kb on + strand at 73.615 kb on + strand at 73.769 kb on + strand at 73.769 kb on + strand at 73.769 kb on + strand at 73.769 kb on + strand at 73.769 kb on + strand at 73.769 kb on + strand at 73.770 kb on - strand at 73.852 kb on + strand at 73.856 kb on - strand at 73.949 kb on + strand at 74.011 kb on + strand, within GFF3303 at 74.161 kb on + strand, within GFF3303 at 74.206 kb on + strand, within GFF3303 at 74.207 kb on - strand, within GFF3303 at 74.207 kb on - strand, within GFF3303 at 74.260 kb on + strand, within GFF3303 at 74.264 kb on + strand, within GFF3303 at 74.264 kb on + strand, within GFF3303 at 74.265 kb on - strand, within GFF3303
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 8.75 MOI remove 68,548 + GFF3299 0.27 -1.0 68,569 + GFF3299 0.31 -0.1 68,664 + GFF3299 0.45 -0.2 68,775 - GFF3299 0.63 -0.4 68,787 - GFF3299 0.65 -3.3 68,890 + GFF3299 0.81 +0.8 68,985 + -0.2 68,985 + +0.0 69,404 + +0.1 69,404 + +0.5 69,448 + -0.3 69,449 - +0.4 69,551 - -0.2 69,553 - -0.2 69,553 - +0.1 69,845 - -0.7 69,845 - +0.4 70,009 + GFF3301 0.14 -0.3 70,010 - GFF3301 0.14 -1.6 70,021 - GFF3301 0.14 -0.9 70,198 - GFF3301 0.19 -0.7 70,209 - GFF3301 0.19 -0.2 70,261 - GFF3301 0.20 -0.3 70,266 + GFF3301 0.20 -0.5 70,267 - GFF3301 0.20 -0.5 70,270 + GFF3301 0.20 -0.4 70,270 + GFF3301 0.20 -0.6 70,270 + GFF3301 0.20 -0.5 70,271 - GFF3301 0.20 +0.3 70,271 - GFF3301 0.20 -0.2 70,338 + GFF3301 0.22 +0.3 70,341 - GFF3301 0.22 -1.1 70,341 - GFF3301 0.22 +0.3 70,616 - GFF3301 0.29 +1.1 70,616 - GFF3301 0.29 -0.5 70,759 - GFF3301 0.33 -0.5 71,412 + GFF3301 0.49 +0.0 71,718 + GFF3301 0.57 -1.2 71,764 + GFF3301 0.58 +0.0 71,772 - GFF3301 0.58 +1.0 71,864 + GFF3301 0.61 +0.0 71,865 - GFF3301 0.61 +1.3 71,904 - GFF3301 0.62 +0.2 71,939 + GFF3301 0.62 -1.1 72,029 + GFF3301 0.65 -0.1 72,179 + GFF3301 0.69 -0.4 72,180 - GFF3301 0.69 -0.9 72,441 + GFF3301 0.75 -0.2 72,465 - GFF3301 0.76 -0.1 72,576 - GFF3301 0.79 +1.4 72,809 - GFF3301 0.84 +1.4 72,809 - GFF3301 0.84 -0.5 72,874 + GFF3301 0.86 +0.0 72,889 - GFF3301 0.86 -0.4 73,268 - +0.4 73,287 + +0.1 73,307 + -0.5 73,308 - -0.4 73,311 + -0.8 73,615 + +0.3 73,769 + +0.0 73,769 + -0.2 73,769 + +1.0 73,769 + +1.3 73,769 + +0.1 73,769 + -0.0 73,770 - -1.4 73,852 + -0.7 73,856 - -0.3 73,949 + +0.7 74,011 + GFF3303 0.11 -0.6 74,161 + GFF3303 0.21 -0.1 74,206 + GFF3303 0.24 -0.1 74,207 - GFF3303 0.24 +0.1 74,207 - GFF3303 0.24 +1.2 74,260 + GFF3303 0.27 -0.3 74,264 + GFF3303 0.28 +0.4 74,264 + GFF3303 0.28 -0.2 74,265 - GFF3303 0.28 -0.7
Or see this region's nucleotide sequence