Experiment: LB_plus_SM_buffer with EIP16_phage 8.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3260 and GFF3261 are separated by 48 nucleotides GFF3261 and GFF3262 overlap by 4 nucleotides GFF3262 and GFF3263 are separated by 86 nucleotides GFF3263 and GFF3264 are separated by 122 nucleotides GFF3264 and GFF3645 are separated by 119 nucleotides GFF3645 and GFF3265 are separated by 138 nucleotides
GFF3260 - AraC family transcription regulator, at 34,603 to 35,391
GFF3260
GFF3261 - Acylphosphate phosphohydrolase (EC 3.6.1.7), putative, at 35,440 to 35,721
GFF3261
GFF3262 - tRNA 2-thiouridine synthesizing protein E (EC 2.8.1.-), at 35,718 to 36,047
GFF3262
GFF3263 - Putative TEGT family carrier/transport protein, at 36,134 to 36,793
GFF3263
GFF3264 - hypothetical protein, at 36,916 to 37,068
GFF3264
GFF3645 - tRNA-Ser-TGA, at 37,188 to 37,275
GFF3645
GFF3265 - Pathogenicity island encoded protein: SPI3, at 37,414 to 37,917
GFF3265
Position (kb) 36
37 Strain fitness (log2 ratio) -1
0
1
2
3 at 35.137 kb on - strand, within GFF3260 at 35.304 kb on - strand, within GFF3260 at 35.389 kb on + strand at 35.394 kb on - strand at 35.394 kb on - strand at 35.394 kb on - strand at 35.402 kb on + strand at 35.423 kb on - strand at 35.502 kb on + strand, within GFF3261 at 35.697 kb on - strand at 35.709 kb on + strand at 35.720 kb on - strand at 36.048 kb on - strand at 36.222 kb on + strand, within GFF3263 at 36.223 kb on - strand, within GFF3263 at 36.251 kb on - strand, within GFF3263 at 36.349 kb on + strand, within GFF3263 at 36.386 kb on - strand, within GFF3263 at 36.404 kb on - strand, within GFF3263 at 36.404 kb on - strand, within GFF3263 at 36.532 kb on - strand, within GFF3263 at 36.532 kb on - strand, within GFF3263 at 36.532 kb on - strand, within GFF3263 at 36.615 kb on + strand, within GFF3263 at 36.616 kb on - strand, within GFF3263 at 37.048 kb on - strand, within GFF3264 at 37.339 kb on - strand at 37.356 kb on + strand at 37.415 kb on + strand at 37.419 kb on + strand at 37.482 kb on + strand, within GFF3265 at 37.482 kb on + strand, within GFF3265 at 37.483 kb on - strand, within GFF3265 at 37.546 kb on + strand, within GFF3265 at 37.698 kb on + strand, within GFF3265
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with EIP16_phage 8.75 MOI remove 35,137 - GFF3260 0.68 -0.4 35,304 - GFF3260 0.89 +0.5 35,389 + -1.2 35,394 - +3.5 35,394 - +0.3 35,394 - +0.7 35,402 + +0.6 35,423 - +0.3 35,502 + GFF3261 0.22 -0.0 35,697 - +0.1 35,709 + -0.3 35,720 - -0.3 36,048 - +0.5 36,222 + GFF3263 0.13 +0.6 36,223 - GFF3263 0.13 -0.6 36,251 - GFF3263 0.18 -0.4 36,349 + GFF3263 0.33 +1.3 36,386 - GFF3263 0.38 +0.2 36,404 - GFF3263 0.41 +0.2 36,404 - GFF3263 0.41 -1.2 36,532 - GFF3263 0.60 +1.5 36,532 - GFF3263 0.60 +0.3 36,532 - GFF3263 0.60 +0.7 36,615 + GFF3263 0.73 -0.3 36,616 - GFF3263 0.73 +0.1 37,048 - GFF3264 0.86 -0.6 37,339 - -0.6 37,356 + -1.2 37,415 + +0.2 37,419 + +1.2 37,482 + GFF3265 0.13 +0.8 37,482 + GFF3265 0.13 +0.3 37,483 - GFF3265 0.14 +3.2 37,546 + GFF3265 0.26 +0.7 37,698 + GFF3265 0.56 -0.3
Or see this region's nucleotide sequence